Qiaxcel connect system

The DNA was extracted from 4 day old seedlings of soybean accessions in two replicates [73 (link)]. The genotyping of the soybean collection was conducted using twenty-five SSR markers (Table S1). These SSRs were selected based on their associations with PH (Table S1). PCR conditions were optimized in order to provide high efficiency and accuracy of amplification [15 (link)]. The PCR was performed in a total volume of 20 µL, comprising 20 ng of genomic DNA, 1 U of Taq polymerase, 0.2 mM of each deoxyribonucleotide triphosphate (dNTP), 10 pM of each primer, 1.5 mM of magnesium chloride (MgCl₂), and a standardized 1× Taq buffer solution. Table S1 summarizes information about the chromosome positions, primers, and motifs of each SSR marker in the analysis.
The PCR products were separated on a QIAxcel Connect System for capillary electrophoresis (QIAGEN, Stockach, Germany) using a QIAxcel DNA High Resolution Kit and QX Alignment Marker (15 bp/3 kb) (Figure S3). The OH500 method was used to run the samples with an injection time of 20 s.

The DNA was extracted from 4 day old seedlings of soybean accessions in two replicates [73 (link)]. The genotyping of the soybean collection was conducted using twenty-five SSR markers (Table S1). These SSRs were selected based on their associations with PH (Table S1). PCR conditions were optimized in order to provide high efficiency and accuracy of amplification [15 (link)]. The PCR was performed in a total volume of 20 µL, comprising 20 ng of genomic DNA, 1 U of Taq polymerase, 0.2 mM of each deoxyribonucleotide triphosphate (dNTP), 10 pM of each primer, 1.5 mM of magnesium chloride (MgCl₂), and a standardized 1× Taq buffer solution. Table S1 summarizes information about the chromosome positions, primers, and motifs of each SSR marker in the analysis.
The PCR products were separated on a QIAxcel Connect System for capillary electrophoresis (QIAGEN, Stockach, Germany) using a QIAxcel DNA High Resolution Kit and QX Alignment Marker (15 bp/3 kb) (Figure S3). The OH500 method was used to run the samples with an injection time of 20 s.