MeONPs were in-house synthetized, donated, or purchased from commercial sources as outlined in Table S1; the in-house synthesis of (George et al. 2011 (link)) and ZnO (Tani et al. 2002 (link)) was conducted by a flame spray pyrolysis reactor as previously described; deionized (DI) water was obtained from a Milli-Q® water purification system (Millipore); THP-1 cells were purchased from ATCC; Corning® RPMI 1640 (CAT 10-041-CV) were purchased from Corning Inc.; 10% fetal bovine serum (CAT 100-500) was purchased from GeminiBio. Phorbol 12-myristate acetate (PMA) and lipopolysaccharide (LPS) were purchased from Sigma-Aldrich. MTS kit was purchased from Promega. Magic cathepsin B assay kit was purchased from Immunochemistry Technologies; , IL-6, , and MCP-1 ELISA kits were purchased from BD Biosciences.
Magic redtm cathepsin b assay kit
Manufactured by ImmunoChemistry Technologies
The Magic RedTM cathepsin B assay kit is a laboratory tool used to detect and quantify the activity of the enzyme cathepsin B. Cathepsin B is a proteolytic enzyme involved in various cellular processes. The kit utilizes a fluorogenic substrate that emits a red fluorescent signal upon cleavage by cathepsin B, allowing for the measurement of enzyme activity.
Automatically generated - may contain errors
Lab products found in correlation
Lipopolysaccharide lps, by Merck Group (1 mentions)
Thp 1 cell, by American Type Culture Collection (1 mentions)
Mts kit, by Promega (1 mentions)
Milli q water purification system, by Merck Group (1 mentions)
Phorbol 12 myristate acetate pma, by Merck Group (1 mentions)
Cellr software, by Olympus (1 mentions)
Ix81 motorized inverted microscope, by Olympus (1 mentions)
2 protocols using magic redtm cathepsin b assay kit
1
Synthesis and Characterization of Metal Oxide Nanoparticles
2
Matrigel-Coated Tumor Spheroid Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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MCTS were prepared according to the hanging-drop method as before [44 (link)]. Formed MCTS were then transferred to wells of Lab-Tek™ Chambered Coverglass which had been coated with 70 μl of Matrigel (5 mg/ml) in SFM. Spheroids were covered with an additional 70 μl of Matrigel (5 mg/ml) in SFM and, after 20 min at 37°C, covered with 400 μl of complete medium. Nitroxoline (2.5 or 5 μM), CA-074 (5 μM), CA-074Me (5 μM) or DMSO (0.05 %) were added to the Matrigel and the medium and the growth of spheroids was monitored for up to three days by measuring the spheroid dimensions under a light microscope, using an ocular micrometer. Spheroid volume was calculated according to the equation: V = (π × (spheroid length) × (spheroid width)2)/6. In a separate experiment, CatB activity was assayed using a Magic RedTM Cathepsin B Assay Kit according to manufacturer's instructions (Immunochemistry Technologies, Bloomington, MN). Images of tumor spheroids were obtained using an Olympus IX 81 motorized inverted microscope and Cell^R software (Olympus, Tokyo, Japan).
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