The studied microorganisms were three strains of bacteria (S. aureus ATCC 25923, E. coli S2 (1) and S. flexneri SDINT) and three strains of yeasts (C. parapsilosis ATCC 22019, C. albicans ATCC 9002 and C. neoformans IP95026) taken from our laboratory collection. The bacterial and fungal species were grown at 37 °C and maintained on nutrient agar (NA, Conda, Madrid, Spain) and Sabouraud Dextrose Agar (SDA, Conda) slants respectively.
Sabouraud dextrose agar
Manufactured by Condalab
Sourced in Spain
Sabouraud Dextrose Agar is a culture medium used for the isolation and cultivation of fungi and yeast. It contains dextrose as the primary carbon source and supports the growth of a wide range of fungal species.
Automatically generated - may contain errors
Lab products found in correlation
Nutrient agar, by Condalab (2 mentions)
Klebsiella pneumoniae, by American Type Culture Collection (1 mentions)
Salmonella typhi, by American Type Culture Collection (1 mentions)
Sabouraud dextrose broth (sdb), by Condalab (1 mentions)
Escherichia coli, by American Type Culture Collection (1 mentions)
Pseudomonas aeruginosa, by American Type Culture Collection (1 mentions)
Staphylococcus aureus, by American Type Culture Collection (1 mentions)
Enterococcus faecalis, by American Type Culture Collection (1 mentions)
Candida albicans, by American Type Culture Collection (1 mentions)
Candida parapsilosis, by American Type Culture Collection (1 mentions)
C albicans atcc 90028, by American Type Culture Collection (1 mentions)
C parapsilosis atcc 22019, by American Type Culture Collection (1 mentions)
Candida tropicalis atcc 750, by American Type Culture Collection (1 mentions)
12 protocols using sabouraud dextrose agar
1
Microorganisms Cultivation Protocol
2
Dermatophyte Inoculum Preparation Protocol
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The inoculum of each dermatophyte was prepared from a 15 days old culture on Sabouraud Dextrose Agar (Conda, Madrid, Spain). The culture surfaces were gently scraped and introduced in test tubes containing 10 mL of sterile saline, homogenized for 5 minutes and filtered. The absorbance of the spore suspensions (filtrates) were read at 530 nm and adjusted with sterile distilled water between 0.15 and 0.17 (Jenway 6105UV/Vis spectrophotometer, 50Hz/60Hz) to match 0.6 × 106 -1.4 × 106 CFU.mL−1 [8 (link)].
3
Antimicrobial Potency Evaluation
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The antimicrobial activity was performed against four bacterial and three fungal species. The selected microorganisms were one Gram-positive Staphylococcus aureus ATCC25923, three Gram-negative Vibrio cholera NB2, V. cholera SG24 and V. cholera CO6 and three yeast strains Candida albicans ATCC10231, Candida tropicalis PK233 and Cryptococcus neoformans H99. These microorganisms were taken from our laboratory collection. The fungal and bacterial strains were grown at 37 °C and maintained on Sabouraud Dextrose Agar (SDA, Conda, Madrid, Spain) and nutrient agar (NA, Conda) slants respectively.
4
Antimicrobial Susceptibility Testing
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Five bacteria and two yeasts were tested for their susceptibility to the studied samples. The studied microorganisms were three Gram-positive (Staphylococcus aureus ATCC25923, methicillin sensitive S. aureus MSSA01 and methicillin resistant S. aureus MRSA03) and two Gram-negative (Pseudomonas aeruginosa ATCC27853, Shigella flexneri SDINT) bacteria and two yeast strains of Candida albicans ATCC10231 and Cryptococcus neoformans H99. These microorganisms were taken from our laboratory collection. The bacterial and fungal species were maintained on agar slant at + 4 °C and on nutrient agar (NA, Conda, Madrid, Spain) and Sabouraud Dextrose Agar (SDA, Conda) slants respectively, prior to any antimicrobial test.
5
Microbial Strain Cultivation and Maintenance
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The microorganisms used in this study include four bacterial (Staphylococcus aureus ATCC25923, methicillin sensitive S. aureus MSSA1, methicillin resistant S. aureus MRSA3 and methicillin resistant S. aureus MRSA4) and three yeast strains (Candida albicans ATCC10231, Candida tropicalis PK233 and Cryptococcus neoformans H99). These microorganisms were taken from our laboratory collection. The fungal and bacterial strains were grown at 37 °C and maintained on Sabouraud Dextrose Agar (SDA, Conda, Madrid, Spain) and nutrient agar (NA, Conda) slants respectively.
6
Antimicrobial Activity Screening Protocol
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The antimicrobial activity was performed against five bacterial and two fungal species. The selected microorganisms were the Gram-positive (Staphylococcus aureus ATCC25923, methicillin-resistant Staphylococcus aureus 03 (MRSA03), and methicillin-resistant Staphylococcus aureus 04 (MRSA04)) and Gram-negative (Pseudomonas aeruginosa ATCC27853 and Escherichia coli S2(1)) bacteria and yeast strains of Candida albicans ATCC10231 and Cryptococcus neoformans H99. These microorganisms were taken from our laboratory collection. The fungal and bacterial strains were conserved on Sabouraud Dextrose Agar (SDA, Conda, Madrid, Spain) and nutrient agar (NA, Conda) slants, respectively.
7
Profiling of Clinically Relevant Microbes
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The studied microorganisms were both reference (from the American Type Culture Collection) and clinical (from Pasteur Institute Paris, France) strains of Enterobacter aerogenes, Escherichia coli, Klebsiella pneumoniae, Candida albicans, and Cryptococcus neoformans. Also, included were two clinical isolates of Candida parapsilosis and Staphylococcus aureus collected from Pasteur Centre (Yaoundé-Cameroon). The bacterial and fungal species were grown at 37°C and maintained on nutrient agar (NA, Conda, Madrid, Spain) and Sabouraud Dextrose Agar (SDA, Conda) slants respectively.
8
Antimicrobial Evaluation of Diverse Microbes
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The microorganisms used in this study were obtained from the American Type Culture Collection (ATCC), “EcoleNationaleVétérinaired’Alfort” (E), “centre Pasteur” of Yaounde-Cameroon and “Institut Pasteur” of Paris-France (IP). They includedeight bacteria strains: Salmonella typhi ATCC 6539, Staphylococcus aureus ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae ATCC 13883, Escherichia coli ATCC 10536, Enterococcus faecalis ATCC 10541, Enterobacter aeroginese ATCC 13048, Providensia smartii ATCC 29916; five yeasts: Candida albicans ATCC 2091, Candida guiliermondii, Cryptococcus neoformans IP 90526, Candida luciteniae ATCC 200950 and Candida parapsilosis ATCC 22019 and five dermatophytes: Trichophyton equinum E1424, Microsporium audouinii E1421, Trichophyton mentagrophytes E1425, Microsporium gypseum E1420 and Epidermophyton flocosum.The culture media, Nutrient Agar (NA, Conda) and Sabouraud Dextrose Agar (SDA, Conda), were used for culturing bacteria and fungi respectively, while Mueller Hinton Broth (MHB, Conda), and Sabouraud Dextrose Broth (SDB, Conda) were used for the determination of minimum inhibitory and minimum microbicidal concentrations.
9
Multidrug Resistance Evaluation of Clinical and Reference Microbes
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The studied microorganisms were both reference (from the American Type Culture Collection) and clinical (from Institut Pasteur and Ecole Nationale Vétérinaire d’Alford, France) strains of Providencia stuartii, Escherichia coli, Pseudomonas aeruginosa, Enterobacter aerogenes, Klebsiella pneumoniae, Candida albicans, Crytococcus neoformans and Trichophyton terrestre. Also, included were two clinical isolates of Trichophyton ajeloi and Trichophyton violaceum, obtained at the Laboratory of Microbiology and Antimicrobial Substances, University of Dschang and two clinical isolates of Candida parapsilosis and Staphylococcus aureus collected from Pasteur Centre (Yaounde-Cameroon). The bacterial and fungal species were grown at 37/28 °C and maintained on nutrient agar (NA, Conda, Madrid, Spain) and Sabouraud Dextrose Agar (SDA, Conda) slants respectively.
10
Characterization of Candida Isolates
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The strains used in this study included 15 Candida albicans (HL 973, HL 963, HL 996, HL 27, HL 3929, HL 3973, HL 3863, HL 3084, HL 3961, HL 17034, HL 3916, HL 3974, HL 3970, HL 3968, and ATCC 90028), 1 Candida glabrata (HL 981), 1 Candida krusei (HL 981), 1 Candida parapsilosis (ATCC 22019), and 1 Cryptococcus tropicalis (ATCC 750). The strains HL were isolated from patients with clinical fungal infection in Changchun QianWei hospital (China). The Candida species were preliminarily identified according to the colored colony morphology on CHRO Magar Candida medium (CHRO Magar Co., Paris, France) which was used to confirm the Candida species. The reference strains, Candida tropicalis ATCC 750, C. parapsilosis ATCC 22,019, and C.albicans ATCC 90,028 were purchased from American Type Culture Collection. All isolates were incubated at 35 °C and subcultured onto Sabouraud dextrose agar (SDA, Conda) at 4 °C in School of Public Health, Jilin University, China.
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