Insulin antibody sc 9168

Antioxidant N-acetyl cysteine (NAC) (Sigma, MO) was used at a working concentration of 5 mM. Ask1 inhibitor NQDI1 (R&D Systems) and JNK inhibitor (JK1) (Abcam) were used at a working concentration of 10 µM. Lactacystin (Lac) (Calbiochem, CA) was used at a concentration of 10 µM. H₂O₂ (Sigma) and diamide (Sigma) were used at working concentrations of 500 µM and 1mM respectively. Anti-PARP (rabbit, sc-7150), anti-actin (goat, sc-1616), anti-ASK1 (goat, sc-6368), anti-phospho-ASK1 (rabbit, sc-1099), anti-caspase-3 (goat, sc-1224), anti-JNK (rabbit, sc-572) and anti-phospo-JNK (goat, sc-12882) polyclonal primary antibodies were purchased from Santa Cruz Biotechnology. Insulin antibody (sc-9168) was purchased from Santa Cruz Biotech. For detection of overexpressed human amylin in transgenic mice islets, mouse monoclonal anti-human antibody (Santa Cruz Biotech., sc-377530) was used. For detection of internalized synthetic human amylin, rabbit polyclonal anti-amylin antibody (Santa Cruz Biotech., CA sc-20936) was used. While both anti-amylin antibodies show high binding affinity toward pro- and pre/pro-human amylin isoforms, only polyclonal anti-hA antibody detects synthetic human amylin (Figure S1A), which was used in amylin internalization studies (Fig. 1C) [41 (link)].