Fosl2

Manufactured by Santa Cruz Biotechnology

FOSL2 is a gene that encodes a transcription factor belonging to the Fos gene family. The protein functions as a regulator of gene expression. This product is intended for use in research applications.

Automatically generated - may contain errors

Lab products found in correlation

Vinculin, by Merck Group (1 mentions) V9131, by Merck Group (1 mentions) Hnf4a, by Santa Cruz Biotechnology (1 mentions) Smc1a, by Fortis Life Sciences (1 mentions) Nipbl, by Fortis Life Sciences (1 mentions) Foxa1, by Santa Cruz Biotechnology (1 mentions) C ebpβ, by Santa Cruz Biotechnology (1 mentions) Med1 a300 793a, by Fortis Life Sciences (1 mentions) H3k27ac, by Abcam (1 mentions)

2 protocols using fosl2

Lentiviral Knockdown of Transcription Factors

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Plasmid catalog numbers and shRNA sequences are provided in Supplementary Table S3. Lentiviruses were produced as previously described58 (link). Cells were transduced with the lentiviruses for 24 h before puromycin selection for the indicated time. Validation of efficiency were performed by Western blot (Supplementary Figs 1 and 3) using MED1 (Bethyl A300-793A, 1:5000), SMC1A (Bethyl, A300-055A, 1:5000), NIPBL, (Bethyl, A301-779A, 1:1000), ERa (Santa Cruz, sc-543x, 1:5000), FOXA1 (Santa Cruz, sc-101058 1:1000), FOXA2 (Abnova, 89-019-034, 1:1000), HNF4a (Santa Cruz, sc-8987x, 1:1000), FOSL2 (Santa Cruz, sc-604x, 1:1000), GAPDH (Pierce, PIMA515738, 1:25000) and Vinculin (SIGMA, V9131, 1:50000).

Fournier M., Bourriquen G., Lamaze F.C., Côté M.C., Fournier É., Joly-Beauparlant C., Caron V., Gobeil S., Droit A, & Bilodeau S. (2016). FOXA and master transcription factors recruit Mediator and Cohesin to the core transcriptional regulatory circuitry of cancer cells. Scientific Reports, 6, 34962.

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ChIP-seq protocol for transcription factor analysis

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ChIP was performed as described previously (Steger et al. 2008 (link)). The following antibodies were used in this study: C/EBPα (sc-61x, Santa Cruz Biotechnology), C/EBPβ (sc-150x, Santa Cruz Biotechnology), FOSL2 (sc-13017x, Santa Cruz Biotechnology), JUND (sc-74x, Santa Cruz Biotechnology), ATF2 (sc-6233x, Santa Cruz Biotechnology), MED1 (A300-793A, Bethyl Laboratories), H3K27ac (ab4729, Abcam), CBP (sc-369x, Santa Cruz Biotechnology), p300 (sc-585x, Santa Cruz Biotechnology), and NCoR (rabbit polyclonal, generated in our laboratory). Primer sequences used for ChIP-qPCR are provided in Supplemental Table S2. ChIP DNA was prepared for sequencing according to the amplification protocol provided by Illumina. Next-generation sequencing of ChIP-seq libraries was performed by the Functional Genomics Core at the University of Pennsylvania, including preprocessing and alignment of the raw tags.

Step S.E., Lim H.W., Marinis J.M., Prokesch A., Steger D.J., You S.H., Won K.J, & Lazar M.A. (2014). Anti-diabetic rosiglitazone remodels the adipocyte transcriptome by redistributing transcription to PPARγ-driven enhancers. Genes & Development, 28(9), 1018-1028.

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