Droperidol

Manufactured by Merck Group

Sourced in United States

Droperidol is a butyrophenone-class medication used as a sedative, antiemetic, and as an adjunct in anesthesia. It is a laboratory reagent used in various research and analytical applications.

Automatically generated - may contain errors

Lab products found in correlation

8 protocols using droperidol

Dopamine Receptor Ligand Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dopamine hydrochloride, (S)-(−)-sulpiride, dimethyl sulfoxide (DMSO), and droperidol were all acquired from Sigma-Aldrich. Haloperidol and piribedil were both acquired from TCI Chemicals. Bromocriptine mesylate was acquired from VWR. Apomorphine hydrochloride was kindly provided by EVERPharma AT GmbH within the context of a different project. Tested compounds (shown in Table S9) were acquired either from SPECS (https://www.specs.net/, accessed in April 2021) or Maybridge (https://www.thermofisher.com/at/en/home/industrial/pharma-biopharma/drug-discovery-development/screening-compounds-libraries-hit-identification.html, accessed in April 2021). All the compounds were dissolved in DMSO, aliquoted, and stored at −80 °C until further use.

Zell L., Lainer C., Kollár J., Temml V, & Schuster D. (2022). Identification of Novel Dopamine D2 Receptor Ligands—A Combined In Silico/In Vitro Approach. Molecules, 27(14), 4435.

+ Open protocol

+ Expand

Immunohistochemistry of Lymphatic and Vascular Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dulbecco’s phosphate-buffered saline (DPBS) (Catalog no. 14040-133, Gibco by Life Technologies Corporation, Grand Island, NY 14072, USA)
D-MEM/F12 (Catalogue number 11039-021, Gibco by Life Technologies Corporation, Grand Island, NY 14072, USA) supplemented with antibiotic mixture (Invitrogen) to achieve a final concentration of 100 units of penicillin/100 μg of streptomycin/mL
Ketamine/xylazine (Catalogue number: K113–10ML, Sigma-Aldrich, St. Louis, MO 63178, USA)
1XProtease inhibitor cocktail (Catalog Number P8340, Sigma-Aldrich)
Fentanyl (Catalogue number F3886, Sigma-Aldrich) / Droperidol (Catalogue Number D1414, Sigma-Aldrich)
Diazepam (Catalogue number NDC0409-3213-12, Hospira Inc., Lake Forest, IL 60045, USA)
ovalbumin-conjugated Alexa Fluor 647 (Thermo Fisher Scientific O34784)
aCSF (Harvard Apparatus 597316)
Superfrost Plus Slides (Thermo Fisher Scientific)
Fisherbrand Cover Glasses 50X22 No 1 (Fisher 12-545E)
Alexa Flour 488-conjugated rat anti-mouse Lyve1 (Invitrogen 14-0443-82, clone ALY7)
Armenian hamster anti-mouse CD31 (Millipore Sigma MAB1398Z, clone 2H8)
Alexa Fluor 594-conjugated goat anti-Armenian hamster (Jackson ImmunoResearch, 127-585-160)
DAPI (Sigma-Aldrich D9542)
ProLong Gold Antifade Mountant (Invitrogen)

Zawieja D.C., Thangaswamy S., Wang W., Furtado R., Clement C.C., Papadopoulos Z., Vigano M., Bridenbaugh E.A., Zolla L., Gashev A.A., Kipnis J., Lauvau G, & Santambrogio L. (2019). Lymphatic Cannulation for Lymph Sampling and Molecular Delivery. Journal of immunology (Baltimore, Md. : 1950), 203(8), 2339-2350.

+ Open protocol

+ Expand

Procuring Bioactive Compounds for Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

Additional supplies of the top 5 hits were ordered from Axon Medchem [Reston, Virginia, USA] (Droperidol—Catalogue ID: 1554) and Sigma-Aldrich [St. Louis, Missouri, USA] (Mesoridazine—Product #: M4068-5MG, Metaproterenol—Product #: M2398-1G, PD 81,723—Product #: P1123-50MG, and Raclopride—Product #: R121-25MG). Indirubin 3’ Monoxime (I3M) was ordered from MilliporeSigma Canada Co. [Product #: I0404, Oakville, Ontario, Canada].

Mauro A.N., Turgeon P.J., Gupta S., Brand-Arzamendi K., Chen H., Malone J.H., Ng R., Ho K., Dubinsky M., Di Ciano-Oliveira C., Spring C., Plant P., Leong-Poi H., Marshall J.C., Marsden P.A., Connelly K.A, & Singh K.K. (2022). Automated in vivo compound screening with zebrafish and the discovery and validation of PD 81,723 as a novel angiogenesis inhibitor. Scientific Reports, 12, 14537.

+ Open protocol

+ Expand

Binding Assay for Antipsychotic Drugs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tag-lite labeling medium (LABMED), SNAP-Lumi4-Tb, and the PPHT ((±)-2-(n-phenethyl-n-propyl)amino-5-hydroxytetralin hydrochloride;1-Naphthalenol,5,6,7,8-tetrahydro-6-[(2-phenylethyl)propylamino]) derivative labeled with a red fluorescent probe (PPHT-red) was obtained from Cisbio Bioassays (Bagnols-sur-Cèze, France). Ninety-six-well polypropylene plates (Corning) were purchased from Fisher Scientific UK (Loughborough, UK) and 384-well optiplate plates were purchased from PerkinElmer (Beaconsfield, UK). GppNHp, risperidone, chlorpromazine hydrochloride, quetiapine hemifumarate, ziprasidone hydrochloride monohydrate, zotepine, sertindole, thioridazine hydrochloride, fluphenazine dihydrochloride, molindone hydrochloride, loxapine succinate, perphenazine, trifluoperazine dihydrochloride, spiperone, (−)-sulpiride, droperidol, and (+)-butaclamol used in competition assays were obtained from Sigma-Aldrich (Poole, UK). Olanzapine, nemonapride, remoxipride hydrochloride, flupenthixol dihydrochloride, paliperidone, amisulpride, melperone hydrochloride, clozapine, raclopride, domperidone, asenapine maleate and haloperidol hydrochloride used for competition assays were obtained from Tocris Bioscience (Avonmouth, Bristol).

Sykes D.A., Moore H., Stott L., Holliday N., Javitch J.A., Lane J.R, & Charlton S.J. (2017). Extrapyramidal side effects of antipsychotics are linked to their association kinetics at dopamine D2 receptors. Nature Communications, 8, 763.

+ Open protocol

+ Expand

Cardiac Toxicity Evaluation of hERG Inhibitors

Check if the same lab product or an alternative is used in the 5 most similar protocols

Drug assays were performed when the rhythmic beating profiles of the cardiomyocytes became stable. For this study, three typical hERG K⁺ channel inhibitors, namely, astemizole (Sigma-Aldrich, USA), droperidol (Sigma-Aldrich, USA), and sertindole, were applied at various doses (16 nM, 80 nM, 400 nM, 2 μM, and 10 μM) to treat the cardiomyocytes. These drugs were prepared in dimethylsulfoxide (DMSO, Sigma-Aldrich, USA) and PBS and then prewarmed in a 37 °C water bath before being added to the culture wells. Mechanical beating signals were recorded before drug treatment as controls.

Wang H., Li H., Wei X., Zhang T., Xiang Y., Fang J., Wu P., Xie X., Wang P, & Hu N. (2021). Recognition of high-specificity hERG K+ channel inhibitor-induced arrhythmia in cardiomyocytes by automated template matching. Microsystems & Nanoengineering, 7, 24.

+ Open protocol

+ Expand

Antipsychotic Drug Cytotoxicity Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The main chemicals used in this study, including FGAs (chlorpromazine, chlorprothixene, clothiapine, droperidol, flupentixol, fluphenazine, haloperidol, levomepromazine, loxapine, pimozide, prochlorperazine, sulpiride, thioridazine, and trifluoperazine) and SGAs (amisulpride, aripiprazole, brexpiprazole, clozapine, lurasidone, olanzapine, paliperidone, quetiapine, risperidone, ziprasidone, and zotepine) were acquired from Sigma-Aldrich (St. Louis, Missouri, USA) and were of highest purity grade. Before usage, the compounds were dissolved in dimethyl sulfoxide (DMSO) at the proper concentrations. Human hepatoma HepaRG™ cells were purchased from Thermo Fisher Scientific (Waltham, Massachusetts, USA). For 2 weeks, frozen cells were defrosted and kept alive in Williams’ E medium (Sigma-Aldrich, St. Louis, Missouri, USA) with 10% FetalClone™ II serum (HycloneTM, GE Healthcare, Chicago, Illinois, USA), 1 × L-glutamine, 5 μg/mL human insulin, and 50 μM hydrocortisone hemisuccinate without antibiotics as supplements. To produce differentiated hepatocyte-like features, the medium was then changed to the aforementioned medium plus 2% DMSO for an additional 2 weeks. Cells were grown at 37°C in a humidified 5% CO₂ environment. P-nitrophenylphosphate (PNPP) was used in an acid phosphatase (ACP) experiment to measure cell viability (29 (link)).

Wu T.Y., Tien N., Lin C.L., Cheah Y.C., Hsu C.Y., Tsai F.J., Fang Y.J, & Lim Y.P. (2023). Influence of antipsychotic medications on hyperlipidemia risk in patients with schizophrenia: evidence from a population-based cohort study and in vitro hepatic lipid homeostasis gene expression. Frontiers in Medicine, 10, 1137977.

+ Open protocol

+ Expand

Synthesis and Characterization of AIBN-DPD Complex

Check if the same lab product or an alternative is used in the 5 most similar protocols

2,2′−Azobisisobutyronitrile (AIBN) and Droperidol (DPD) were purchased from Merck (Vienna, Austria). 2,2,6,6−tetramethyl−1−piperinyloxy silica gel (SiliaCAT^TM TEMPO) was purchased from Silicycle Inc. (Quebec, Canada), while PVP K−60 (with an average molecular weight of 360,000 Da) was procured from Sigma Aldrich (Vienna, Austria). Ultrapure water was obtained from a TKA water purification unit. HPLC grade Methanol was procured from VWR Chemicals (Dresden, Germany). Buffer salts and other chemicals were of analytical reagent grade.

Iyer J., Saraf I., Ray A., Brunsteiner M, & Paudel A. (2022). Assessment of Diverse Solid−State Accelerated Autoxidation Methods for Droperidol. Pharmaceutics, 14(6), 1114.

+ Open protocol

+ Expand

Accelerated Oxidation Stability Evaluation of Drugs

Check if the same lab product or an alternative is used in the 5 most similar protocols

N-methyl pyrrolidone (NMP) was procured from Sigma Aldrich (Steinheim, Germany). Five of the drug compounds namely, droperidol (DPD), mifepristone (MIF) cimetidine (CIM), naproxen (NAP), clotrimazole (CLO) were purchased from Merck (Sigma Aldrich^®, Vienna, Austria), while indomethacin (IMC), nifedipine (NIF), and diclofenac (DIC) were obtained from TCI chemicals^® (Tokyo, Japan). Olanzapine (OLA) was purchased from Dr. Reddy’s laboratories (Hyderabad, India). All the selected drugs had a chemical purity above 99.90%, and their chemical structures are shown in Figure 1. Polyvinylpyrrolidone (PVP) K-60 was procured from Merck (Sigma Aldrich^®, Vienna, Austria). The weighing of solid powders was done using a laboratory analytical balance. Ultrapure water was obtained from a TKA water purification unit (Vienna, Austria). Accelerated oxidation stability was conducted using a high temperature/pressure oxidation testing device, RapidOxy^® (Anton Paar GmbH^®, Graz, Austria).

Iyer J., Karn A., Brunsteiner M., Ray A., Davis A., Saraf I, & Paudel A. (2023). Screening Autoxidation Propensities of Drugs in the Solid-State Using PVP and in the Solution State Using N-Methyl Pyrrolidone. Pharmaceutics, 15(3), 848.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!