Apc conjugated ccr2

Manufactured by R&D Systems

Sourced in United Kingdom

APC-conjugated CCR2 is a fluorescently labeled antibody for the detection of the chemokine receptor CCR2 by flow cytometry. CCR2 is a G protein-coupled receptor that binds to the chemokine MCP-1 and plays a role in the recruitment of monocytes to sites of inflammation.

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Lab products found in correlation

Dnase 1, by Merck Group (2 mentions) Collagenase 1, by Merck Group (2 mentions) Calibrite, by BD (2 mentions) Collagenase xi, by Merck Group (2 mentions) Hyalronidase, by Merck Group (2 mentions) Pe cy7 conjugated cd45, by BD (2 mentions) Total percp conjugated cd11b, by BD (2 mentions) Pe conjugated cd14, by Thermo Fisher Scientific (2 mentions) Cyan analyser flow cytometer, by Beckman Coulter (2 mentions) Lsrfortessa flow cytometer, by BD (1 mentions) Pacific blue conjugated ly6c, by BioLegend (1 mentions) Fitc conjugated anti cd19, by BioLegend (1 mentions) Apc conjugated cd11c, by BioLegend (1 mentions) Pe conjugated f4 80, by BioLegend (1 mentions) Percp cy5.5 conjugated cd11b, by BioLegend (1 mentions)

Spelling variants of this product

CCR2-APC (7 citations) Mouse CCR2 APC-conjugated antibody (2 citations) APC conjugated anti-CCR2 (2 citations)

3 protocols using apc conjugated ccr2

Aortic Cell Isolation and Flow Cytometry

Cited 1 time

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Descending aortas from the aortic arch to femoral bifurcations were microdissected and digested in an enzyme solution containing 60 U ml⁻¹ DNase I, 60 U ml⁻¹ Hyalronidase, 450 U ml⁻¹ Collagenase I and 125 U ml⁻¹ Collagenase XI (all enzymes from Sigma-Aldrich) at 37 °C (ref. 18 (link)). A single-cell suspension was prepared by passing aortic pieces through a strainer for subsequent flow cytometry staining. Isolated aortic cells were antibody stained for the surface markers PE-Cy7-conjugated CD45 (BD Pharmingen), total PerCP-conjugated CD11b (BD Pharmingen), PE-conjugated CD14 (eBioscience, UK), APC-conjugated CCR2 (R&D Systems, UK) with appropriate isotype controls19 (link) (Supplementary Table 5). Absolute cell counts were performed by ratio to a known quantity of calibration beads added to each sample (CaliBrite, BD Pharmingen). Data were acquired using a CyAn Analyser flow cytometer (Beckman Coulter, UK) and then analysed using Summit (Dako, UK) and FlowJo (Tree Star Inc, USA) software.

Patel J., McNeill E., Douglas G., Hale A.B., de Bono J., Lee R., Iqbal A.J., Regan-Komito D., Stylianou E., Greaves D.R, & Channon K.M. (2015). RGS1 regulates myeloid cell accumulation in atherosclerosis and aortic aneurysm rupture through altered chemokine signalling. Nature Communications, 6, 6614.

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Aortic Cell Isolation and Characterization

Cited 1 time

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Descending aortas from the aortic arch to femoral bifurcations were micro-dissected and digested in an enzyme solution containing 60 U/ml DNase I, 60 U/ml Hyalronidase, 450 U/ml Collagenase I and 125 U/ml Collagenase XI (all enzymes from Sigma-Aldrich, UK) at 37°C^{18 (link)}. A single cell suspension was prepared by passing aortic pieces through a strainer for subsequent flow cytometry staining. Isolated aortic cells were antibody stained for the surface markers PE-Cy7 conjugated CD45 (BD Pharmingen, UK), total PerCP conjugated CD11b (BD Pharmingen, UK), PE conjugated CD14 (eBioscience, UK), APC conjugated CCR2 (R&D systems, UK) with appropriate isotype controls^{19 (link)}(Supplementary Table 5). Absolute cell counts were performed by ratio to a known quantity of calibration beads added to each sample (CaliBrite, BD Pharmingen, UK). Data was acquired using a CyAn Analyser flow cytometer (Beckman Coulter Ltd, UK) and then analyzed using Summit (Dako, UK) and FlowJo (Tree Star Inc, USA) software.

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Hepatic Leukocyte Immunophenotyping by Flow Cytometry

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Freshly isolated hepatic leukocytes (1 Â 10 6 ) were blocked with 2.4G2 (BD Biosciences, San Diego, CA) for 10 minutes before 30 minutes of staining with the following antibodies purchased from BioLegend (San Diego, CA): FITC-conjugated anti-CD19 (1/100 dilution, clone 6D5), PerCP/Cy5.5-conjugated CD11b (1/100 dilution, clone M1/70), AF647-conjugated TIM4 (1/100 dilution, clone RMT4-54), Pacific Blue-conjugated Ly6C (1/ 100 dilution, clone HK1.4), APC-conjugated CD11c (1/100 dilution, clone N418), APC/Cy7-conjugated Ly6G (1/100 dilution, clone 1A8), PE-conjugated F4/80 (1/100 dilution, clone BM8), PE-CF594-conjugated CD3 (1/100 dilution, clone 17A2), and PE/Cy7-conjugated I-A/I-E (1/300 dilution, clone M5/114.15.2). APC-conjugated CCR2 (1/100 dilution, clone 475301) was purchased from R&D Systems (Minneapolis, MN). Live cell events were measured on an LSR Fortessa flow cytometer (BD Biosciences) and analyzed with FlowJo software version 10 (Tree Star, Ashland, OR). The absolute number of each cell type was calculated by multiplying the frequency by the total number of viable hepatic leukocytes per liver.

Melino M., Gadd V.L., Alexander K.A., Beattie L., Lineburg K.E., Martinez M., Teal B., Le Texier L., Irvine K.M., Miller G.C., Boyle G.M., Hill G.R., Clouston A.D., Powell E.E, & MacDonald K.P. (2016). Spatiotemporal Characterization of the Cellular and Molecular Contributors to Liver Fibrosis in a Murine Hepatotoxic-Injury Model. The American journal of pathology, 186(3).

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