Lc 20at solvent delivery module

Manufactured by Shimadzu

Sourced in Japan

The LC-20AT Solvent Delivery Modules are a core component of Shimadzu's liquid chromatography systems. These modules are responsible for the accurate and precise delivery of solvents to the chromatographic system, ensuring consistent and reliable separation of chemical components.

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LC-20AT (490 citations)

5 protocols using lc 20at solvent delivery module

Characterization of Organic Compounds

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Optical rotations were measured on a Jasco-DIP-700 polarimeter at the sodium line (589 nm). UV spectra were obtained on a Hewlett-Packard 8453 spectrophotometer, and IR spectra were measured as a thin film on a CaF₂ disc using a Perkin Elmer 1600 series FTIR. All 1D and 2D NMR spectra with the exception of ¹³C NMR spectra were acquired on a Varian Unity Inova 500 MHz spectrometer operating at 500 (¹H) or 125 (¹³C) MHz using the residual solvent signals (δ_H 7.26 and δ_C.77.0) as an internal reference. ¹³C NMR spectra were acquired on a Varian Unity 600 MHz spectrometer. NMR samples were analyzed in 3 mm Shigemi NMR tubes. High-resolution mass spectrometry (HRMS) data were obtained on an Agilent 6545 LC-MS Q-ToF with ESI ionization in the positive mode. Gradient HPLC separations used a Shimadzu system consisting of LC-20AT Solvent Delivery Modules, an SPDM20A VP Diode Photodiode Array Detector, and an SCL-20A VP System Controller.

Gurr J.R., O’Donnell T.J., Luo Y., Yoshida W.Y., Hall M.L., Mayer A.M., Sun R, & Williams P.G. (2020). 6-Deoxy- and 11-Hydroxytolypodiols: Meroterpenoids from the Cyanobacterium HT-58–2. Journal of natural products, 83(5), 1691-1695.

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Spectroscopic Characterization of Organic Compounds

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Optical rotations were measured on a Jasco-DIP-700 polarimeter at the sodium line (589 nm). UV spectra were obtained on a Hewlett-Packard 8453 spectrophotometer and IR spectra were measured as a thin film on a CaF₂ disc using a Perkin Elmer 1600 series FTIR. ECD measurements were obtained on a Chirascan Circular Dichroism Spectrometer with the samples dissolved in MeOH and placed in a 1 cm quartz cuvette with a solvent subtraction for baseline correction. NMR spectra were acquired on a Varian Inova Unity 500 MHz spectrometer operating at 500 (¹H) or 125 (¹³C) MHz using the residual solvent signals as an internal reference. Samples were in 3 mm Shigemi tubes during NMR analyses. High-resolution mass spectrometry data were obtained on an Agilent LC-TOF or LC-QTOF with ES ionization. Gradient separations used a Shimadzu system consisting of LC-20AT Solvent Delivery Modules, an SPD-M20A VP Diode Photodiode Array Detector, and a SCL-20A VP System Controller. TLC analyses were performed on Si₆₀F₂₅₄ plates and visualized under UV or by heating after spraying with a 1% anisaldehyde solution in acetic acid:H₂SO₄ (50:1). Samples were weighted on a Mettler Toledo analytical balance.

Dai J., Philbin C.S., Wakano C., Yoshida W.Y, & Williams P.G. (2023). New Nostocyclophanes from Nostoc linckia. Marine Drugs, 21(2), 101.

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Liquid Chromatography Analytical Protocol

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Liquid chromatographic system (Shimadzu Corporation, Japan) equipped with dual LC-20 AT solvent delivery modules connected with DGU-20A3/20A5 on-line degasser, tted with rheodyne manual injector connected with SPD-20A/20AV UV/VIS detector and Shimadzu CBM-20A communication bus module.
Data acquisition was performed on LC solution GPC Chromatographic software (version 1.25). λ max was measured on Shimadzu-1800 double beam UV/vis spectrophotometer. Rotary evaporator (Heidolph G 3 Germany) was used to concentrate the sample.

Khalid A., Ali S.N., Qayoom A., Iqbal S., Ansari S., Zahoor A., Kishwar F., & Daniel P. (2021). High Performance Liquid Chromatography – Ultraviolet Method for the Determination of Fludioxonil Fungicide Residues: Application on Rice Grains Cultivated in Pakistan.

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Isolation of Bioactive Compounds by VLC and HPLC

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Fractions of crude extracts H and E were obtained through vacuum liquid chromatography (VLC) on silica gel (0.040–0.063 mm, Merck, Darmstadt, Germany) with solvent mixtures of increasing polarity (hexane, ethyl acetate, methanol, all Roth, Germany). Fractions were controlled by thin-layer chromatography (silica gel 60 F₂₅₄, Merck, Darmstadt, Germany) and those of similar substance patterns combined to yield five fractions for H (H1–H5) and 9 fractions for E (E1–E9).
Four compounds were isolated by semi-preparative HPLC using a Shimadzu CBM-20A controller, LC-20AT solvent delivery module, SIL-10AF autosampler, CTO-20AC column oven, SPD-M20A diode array detector, and FRC-10A fraction collector (all Shimadzu, Kyoto, Japan).
All separations were performed on a Luna C18(2) column, 250 × 10 mm, 10 μm (Phenomenex, Torrance, CA, USA) via isocratic elution with a flow rate of 4 mL/min, 25 °C column temperature; 200 μL injection volume.
Compounds 1 and 7 were obtained from E8 (VLC fraction with hexane:ethyl acetate:methanol = 4:80:16) by isocratic elution with a solvent composition of acetonitrile:water = 70:30 (v/v). Compound 4 was isolated with acetonitrile:water = 63:37 (v/v) from H4 (hexane:ethyl acetate:methanol = 36:60:4) and compound 6 with acetonitrile:water = 75:25 (v/v) from E2 (hexane:ethyl acetate = 70:30).

Šimunović K., Solnier J., Alperth F., Kunert O., Možina S.S, & Bucar F. (2021). Efflux Pump Inhibition and Resistance Modulation in Mycobacterium smegmatis by Peucedanum ostruthium and Its Coumarins. Antibiotics, 10(9), 1075.

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HPLC-UV Analysis of Corticosteroids

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The HPLC-UV system consisted of a Shimadzu (Shimadzu Corporation, Milano, Italy) LC-20AT solvent delivery module equipped with a DGU-20A3 degasser and interfaced with an SPD-20A UV detector. The wavelength selected for analysis was 238 nm that is included in the absorption band of all the GCs. Each sample was diluted (30% v/v) with MeOH and injected (20 µL) into a 250 × 4.6 mm, 5 µm GraceSmart RP18 (Sepachrom) column, coupled with a similar guard-column. Isocratic elution was carried out for 20 min using ultrapure water–ACN mixture, 70:30 for CORT, HCORT, PRED and PREDLO; 65:35 for BETA, DEXA and TRIAM. After washing for 5 min with 100% ACN, the initial conditions were reestablished. The flow rate was 1.0 mL min⁻¹. The instrumental quantification limits were 0.3 mg L⁻¹ for CORT, HCORT and DEXA, 0.09 mg L⁻¹ for BETA, 0.2 mg L⁻¹ for PRED, PREDLO and TRIAM.

Cantalupi A., Maraschi F., Pretali L., Albini A., Nicolis S., Ferri E.N., Profumo A., Speltini A, & Sturini M. (2020). Glucocorticoids in Freshwaters: Degradation by Solar Light and Environmental Toxicity of the Photoproducts. International Journal of Environmental Research and Public Health, 17(23), 8717.

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