Cd4 bv510

Manufactured by Thermo Fisher Scientific

Sourced in France

CD4-BV510 is a fluorescently-labeled monoclonal antibody designed for the detection and quantification of CD4-positive cells in flow cytometry applications. It specifically binds to the CD4 receptor expressed on the surface of T helper cells.

Automatically generated - may contain errors

Lab products found in correlation

7 protocols using cd4 bv510

Lung Cell Isolation and Immunophenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols

Single-cell suspensions of the lung tissues were prepared by cutting them into small pieces followed by incubation in Dulbecco’s Modified Eagle Media (DMEM) containing 0.18 mg/mL Collagenase Type I (Sigma, St. Louis, MO, USA), 0.02 mg/mL DNase I (Sigma, St. Louis, MO, USA) for 1 h at 37 °C under constant rotation, followed by being mechanically passed through a 100 μm and 70 μm cell strainer sequentially. Erythrocytes were lysed using RBC lysis buffer (155 mM NH₄Cl, 12 mM NaHCO₃, 0.1 mM EDTA). Cells were then counted and subjected to flow cytometry. Lymphoid and myeloid compartments were investigated in the lung samples of mice on various intervention diets. Antibodies used for flow cytometry analysis were as follows: CD64-PeCy7 (Clone X54-5/7.1), Ly6C-PerCPCy5.5 (Clone AL-21), CD11b-V450 (Clone M1/70), MHCII-APC (Clone M5/114.15.2), CD103-PE (Clone M290), CD11c-A700 (Clone HL3), SiglecF-APCCy7 (Clone E5-2440), Ly6G-FITC (Clone 1A8), PD-1-FITC (Clone 29F.1A12), CD4-BV510 (Clone RM4-5), CD44-PE (Clone IM7), NK1.1-APCCy7 (Clone PK136), CD3-A700 (Clone 500A2), CD62L-V450 (Clone MEL-14), CD19-PerCPCy5.5 (Clone 1D3), CD8-APC (Clone 53-6.7), and KLRG1-BV786 (Clone 2F1) purchased from BD (Biosciences, Johannesburg, SA) and eBioscience (ThermoFisher, Johannesburg, SA) [29 (link),30 (link)].

Nienaber A., Baumgartner J., Dolman R.C., Ozturk M., Zandberg L., Hayford F.E., Brombacher F., Blaauw R., Parihar S.P., Smuts C.M, & Malan L. (2020). Omega-3 Fatty Acid and Iron Supplementation Alone, but Not in Combination, Lower Inflammation and Anemia of Infection in Mycobacterium tuberculosis-Infected Mice. Nutrients, 12(9), 2897.

+ Open protocol

+ Expand

Mass Cytometry Validation of Blood Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

Samples used for mass cytometry validation were 200 μL of whole blood that were fixed in 1-step Fix/Lyse Solution (eBioscience), and stored at −80 °C. Fixed cells were thawed, permeabilized using Permeabilization Buffer (eBioscience) and then stained with the following antibodies specific for epitopes insensitive to fixation, purchased from Biolegend unless otherwise indicated: CD38 BB515 (HIT2, BD Biosciences), CD4 BV510™ (SK3), CD8 PerCP/Cy5.5 (HIT8a), CD161 APC (HP-3G10, eBioscience), HLA-DR APC/Cy7 (L243), TCR Vα7.2 BV605™ (3C10), TCR γ/δ BV421™ (B1), Ki67 PE (Ki-67), and CD3 BV650™ (UCHT1).

Howson L.J., Napolitani G., Shepherd D., Ghadbane H., Kurupati P., Preciado-Llanes L., Rei M., Dobinson H.C., Gibani M.M., Teng K.W., Newell E.W., Veerapen N., Besra G.S., Pollard A.J, & Cerundolo V. (2018). MAIT cell clonal expansion and TCR repertoire shaping in human volunteers challenged with Salmonella Paratyphi A. Nature Communications, 9, 253.

+ Open protocol

+ Expand

Comprehensive Cytokine Analysis of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

For cytokines analysis, spleen cell (2 × 10⁷cells/well) was seeded on the microplates. The B cells were detected by incubating the samples with anti-mouse CD16/32 (eBioscience, USA) to block the Fc fragment, the antibodies for cell surface proteins, including CD45R-eFluor 450, CD38-FITC, CD138- PC, and GL7-PE (eBioscience) were added for 30 min in the dark. The T cell & T cell cytokines were detected by culturing the spleen cells with the peptide library for 5 h and adding the antibodies for cell surface proteins, including CD3-AF700, CD4-BV510, CD8a-FITC (eBioscience), for 30 min in the dark. After cell fixation with an IC buffer overnight at 4°C, the antibodies for cytokines, including TNF-α-eFluor450, IL-2-APC, IL-4-BV711, and IL-17A-PE/Cy7 (eBioscience), were added and incubated for 1 h in the dark. The cells underwent flow cytometry analysis (Attvne NXT; Thermo Fisher Scientific). FlowJo software (FlowJo, LLC, USA) was used for data analysis.

Zhang J., Ge J., Li J., Li J., Zhang Y., Shi Y., Sun J., Wang Q., Zhang X, & Zhao X. (2023). Expression of FMD virus-like particles in yeast Hansenula polymorpha and immunogenicity of combine with CpG and aluminum adjuvant. Journal of Veterinary Science, 24(1), e15.

+ Open protocol

+ Expand

Comprehensive Immune Cell Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Splenocytes were prepared as described above. Cells were then incubated with the appropriate antibody at 4°C for 30 min in the dark in PBS with 2% FBS. Flow cytometry was performed using a FACS Fortessa II flow cytometer (BD Biosciences), according to standard techniques. For the characterization of splenic cells, the monoclonal antibodies used were: CD3-AF700 (17A2, #100216), CD4-BV510 (GK1.5, #100449), CD8-AF647 (53-6.7, #100727), CD25-PE conjugated (PC61.5, #12-0251-81), B220-BV421 (RA3-6B2, #562922), CD62l-PeCy5 (MEL-14, #104410), CD44-BV605 (IM7, #103047), CD11b-PerCPcy5.5 (M1/70, #101228), CD80-PE (16-10A1, #09605B), F4/80-BV711 (BM8, #123147), CD86-FITC (GL-1, #105005), CD206-AF647 (MR5D3, #565250), CD43-BV421 (S7, #562958), CD11c-AF700 (N418, #117320), MHCII-EFluor480 (M5/114.15.2, #48-5321-82) from eBiosciences (Thermo Fisher Scientific, Villebon-Sur-Yvette, France). Data were analyzed with FlowJo software (Tree Star, Ashland, OR).

Chêne C., Jeljeli M.M., Rongvaux-Gaïda D., Thomas M., Rieger F., Batteux F, & Nicco C. (2022). A Fenton-like cation can improve arsenic trioxide treatment of sclerodermatous chronic Graft-versus-Host Disease in mice. Frontiers in Immunology, 13, 917739.

+ Open protocol

+ Expand

Splenocyte Characterization by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Splenocytes were prepared as described above. Cells were incubated with the appropriate labelled antibody at 4°C for 30 minutes in PBS with 2% FBS. Flow cytometry was performed on a FACS Fortessa II flow cytometer (BD Biosciences) according to standard techniques. For the characterization of splenocytes, the monoclonal antibodies used were: CD3-AF700 (17A2, #100216), CD4-BV510 (GK1.5, #100449), CD25-PE (PC61.5, #12-0251-81), B220-Pacific Blue (RA3-6B2, #103230), CD69-PE DAZZLE (H1.2F3, #104536), CD11b-PerCP Cy5.5 (M1/70, #101228), F4/80-BV711 (BM8, #123147), CD86-BV510 (GL-1, #105039), from eBiosciences (ThermoFisher Scientific, France). Data were analyzed with FlowJo software (Tree Star, Ashland, OR, USA).

Chêne C., Rongvaux-Gaïda D., Thomas M., Rieger F., Nicco C, & Batteux F. (2023). Optimal combination of arsenic trioxide and copper ions to prevent autoimmunity in a murine HOCl-induced model of systemic sclerosis. Frontiers in Immunology, 14, 1149869.

+ Open protocol

+ Expand

Radiation-Induced DNA Damage in Immunodeficiency

Check if the same lab product or an alternative is used in the 5 most similar protocols

PBMCs from n = 11 controls, n = 32 CVID and n = 2 PAD patients were either untreated or exposed to 5 Gy of γ-irradiation and cultured for 1 or 24 h. Cells were stained with Fixable Live/Dead eF780 (ThermoFisher), CD3-BV785 (clone: SK7), CD4-BV510 (SK3), CD8-PE-Cy7 (HIT8a) and CD19-BV605 (HIB19) in PBS, 5% bovine serum albumin, and 0.09% sodium azide for 10 min at room temperature. Cells were washed and fixed with eBioscience FoxP₃/Transcription Factor Staining Buffer Set fixative (eBioscience) for 1 h at 4 °C. Cells were permeabilized in the presence of γH2AX^Ser139-FITC (2F3), ATM^Ser1981-PE (10H11.E12), 53BP1-AF700 (cat. no. NB100-904; Novus Biologics) and cleaved PARP^Asp214-PE-CF594 (F21-852, BD Biosciences) for 1 h at 4 °C. Antibodies were obtained from Biolegend unless otherwise stated. Samples were analyzed using an LSR Fortessa (BD Biosciences) and FlowJo version 9 software (Tree Star).

Hargreaves C.E., Salatino S., Sasson S.C., Charlesworth J.E., Bateman E., Patel A.M., Anzilotti C., Broxholme J., Knight J.C, & Patel S.Y. (2021). Decreased ATM Function Causes Delayed DNA Repair and Apoptosis in Common Variable Immunodeficiency Disorders. Journal of Clinical Immunology, 41(6), 1315-1330.

+ Open protocol

+ Expand

Functional Characterization of T Cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total CD3+ T cells were cotransduced with CAR lentivirus and either pTRIP-SFFV-GFP or pTRIP-SFFV-GFP-RELA K5R for 4 d prior to staining. 0.15 million cells were treated with cell-stimulation cocktail and protein transport inhibitors (#15516286 or 15172069; Thermo Fisher Scientific) for 4 h. Cells were stained with Fixable Viability Dye eFluor 780 (#15383562; Thermo Fisher Scientific), CD4 BV510 (#562971; Thermo Fisher Scientific), and CD8a PE (#15506706; Thermo Fisher Scientific), and then fixed and permeabilized using the eBioscience FOXP3 transcription factor staining buffer set (#11500597; Thermo Fisher Scientific). Permeabilized cells were stained with antibodies against IFNγ PeCy7 (#13417646; Thermo Fisher Scientific) and Granzyme B APC (#515405; Biolegend). Samples were acquired on a NovoCyte (Agilent). Isotype controls were used to define gates.

Jeremiah N., Ferran H., Antoniadou K., De Azevedo K., Nikolic J., Maurin M., Benaroch P, & Manel N. (2023). RELA tunes innate-like interferon I/III responses in human T cells. The Journal of Experimental Medicine, 220(5), e20220666.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!