Long evans

At Dart Neuroscience (DN), 11 adult male Long-Evans (Envigo Laboratories Huntingdon, UK; 300-400g at start of study) were used in Expt. 1A. Rats were housed 2/cage, food-restricted to 85-90% of the free-feeding weight (adjusted for growth), had free access to water and maintained on a 12:12 h light/dark cycle with training conducted during the light phase. All experimental methods were approved by the DN Institutional Animal Care and Use Committee, and followed the guidance of the National Research Council Guide for the Care and Use of Laboratory Animals Studies (2011).
At Edinburgh (EDI), a total of 32 adult male Lister-hooded rats were used in Expt. 1B (n=7; Protocol 1), Expt. 2 (n=8; Protocol 2) and Expts. 3A and 3B (n=17; Protocol 3). As described in detail in Nonaka et al. (2017) (link), they were group housed (3-4/cage; and similarly food-deprived to 85-90% of the free-feeding weight against a growth curve, with free-access to water, 12:12 h light-dark cycle with training in the light phase). Care of the animals complied with the UK Animals (Scientific Procedures) Act conducted under a Project Licence (PPL 60/4566).

Adult male Long-Evans (Envigo, Indianapolis, IN; n=32), Sprague Dawley (Envigo, Frederick, MD; n=16), and Wistar (Charles River, Raleigh, NC; n=32) rats were P60 upon arrival and allowed to acclimate to the vivarium for at least one week before experiments began. All rats were singly housed in standard plexiglass cages in a temperature-controlled room on a reverse light/dark cycle (lights on at 22:00). Teklad 7912 standard chow (Envigo) and water were provided ad libitum unless otherwise indicated. Figure 1 depicts the experimental procedures described below. All experiments were approved by the University of Illinois at Chicago Institutional Animal Care and Use Committee and adhered to the NIH Guidelines for the Care and Use of Laboratory Animals.