Small interfering (si)-KCNQ1OT1, si-negative control (NC), miR-370-3p mimics, mimics-NC, miR-370-3p inhibitor, inhibitor-NC, pcDNA-FOXM1, and pcDNA-NC were purchased from RiboBio (Guangzhou, China). The above factors were transfected into WI-38 cells using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA) for 48 h in accordance with the user guide.
Mir 370 3p mimic
Manufactured by RiboBio
Sourced in China
The MiR-370-3p mimic is a synthetic RNA molecule designed to mimic the function of the naturally occurring microRNA, miR-370-3p. MicroRNAs are small, non-coding RNA molecules that play a role in regulating gene expression. The MiR-370-3p mimic can be used in research applications to study the biological functions and pathways associated with this specific microRNA.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 3000, by Thermo Fisher Scientific (3 mentions)
Mir 370 3p inhibitor, by RiboBio (2 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)
Si circitga7, by GenePharma (2 mentions)
Si nc, by GenePharma (2 mentions)
Mimic nc, by RiboBio (2 mentions)
Inhibitor nc, by RiboBio (1 mentions)
Mimics nc, by RiboBio (1 mentions)
Si kcnq1ot1, by RiboBio (1 mentions)
Pcdna nc, by RiboBio (1 mentions)
Pcdna foxm1, by RiboBio (1 mentions)
Control mirna mimics, by RiboBio (1 mentions)
Control shrna, by Genechem (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Mcf 7, by American Type Culture Collection (1 mentions)
Hcc1937, by American Type Culture Collection (1 mentions)
Mda mb 453, by American Type Culture Collection (1 mentions)
Hbl 100, by American Type Culture Collection (1 mentions)
5 protocols using mir 370 3p mimic
1
Regulation of KCNQ1OT1 by miR-370-3p
2
Plasmid and microRNA transfection protocol
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Overexpressed TUG1 plasmid (pcDNA-TUG1), TUG1 shRNA, control plasmid (pcDNA-NC), and control shRNA were constructed by GeneChem (Shanghai, China). miR-370-3p mimics, miRNA 370-3p inhibitors, and miRNA mimics control were purchased from RiboBio (Guangzhou, China). By applying LipofectamineTM 2000 (Invitrogen, Carlsbad, CA, US) according to the instructions, we transfected plasmid, shRNA or microRNA mimics into cells.
3
Modulating circITGA7 Expression
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To artificially upregulate or downregulate the expression of circITGA7, the pcD-ciR overexpression vector (circITGA7), empty vector (vector), small interfering RNA targeting circITGA7 (si-circITGA7), and negative control (si-NC) were purchased from GenePharma (Shanghai, China). miR-370-3p mimic and its corresponding negative control (mimic-NC) were purchased from RiboBio (Guangzhou, China). All the vectors were transfected into cells with Lipofectamine 3000 (Thermo Fisher Scientific, USA) following the manufacturer's instructions.
4
Breast Cancer Cell Line Cultivation
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Human breast cancer cell lines (MCF-7, MDA-MB-453, MDA-MB-231, and HCC-1937) and a normal mammary epithelial cell line (HBL-100) were purchased from American Type Culture Collection (Manassas, VA, USA). All cells were cultured in Dulbecco’s modified Eagle’s medium (Gibco, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin/streptomycin (Invitrogen, Carlsbad, CA, USA) in a humidified atmosphere with 5% CO2 at 37 °C. Cells have been authenticated by STR profiling and tested for mycoplasma contamination. MiR-370-3p mimic, miR-370-3p inhibitor, their respective controls, small interfering RNA (siRNA) against FGF14 expression (si-FGF14), and negative control siRNA (si-NC) were chemically synthesized by RiboBio (Guangzhou, China). The FGF14-overexpressing plasmid pCMV3-FGF14-Flag and empty vector were from Sino Biological Inc. (Beijing, China). Cells were plated in 6-well plates at 60–70% confluence one day prior to transfection. Cell transfection was conducted using Lipofectamine 2000 (Invitrogen) in accordance with the manufacturer’s instructions. Table S2 lists the siRNA sequences.
5
Circulating ITGA7 Regulation
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To arti cially upregulate or downregulate the expression of circITGA7, the pcD-ciR overexpression vector (circITGA7), empty vector (vector), small interfering RNA targeting circITGA7 (si-circITGA7) and negative control (si-NC) were purchased from Genepharma (Shanghai, China). miR-370-3p mimic and its corresponding negative control (mimic-NC) were purchased from RiboBio (Guangzhou, China). All the vectors were transfected into cells with Lipofectamine 3000 (Thermo Fisher Scienti c, USA) following the manufacturer's instructions.
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