The method for detection of histological changes has been described in a previous study [24 (link)]. The fixed samples were dehydrated, cleaned, embedded in paraffin, and sliced to 5 μm slices by use of an RM2235 microtome (Leica, Munich, Germany). Sliced samples were flattened and dried on glass slides, stained with hematoxylin and eosin (Thermo, Waltham, MA, USA), and sealed with neutral resin. The histopathological changes were observed by a CX22 microscope (Olympus, Tokyo Metropolitan, Japan) and a DM1000 microimaging system (Leica, Munich, Germany) was applied for image recording. In pathological diagnosis, we used INHAND criteria (Table 1 ) to judge the severity of histological lesions [25 (link)].
Dm1000 microimaging system
Manufactured by Leica
Sourced in Japan
The Leica DM1000 is a microimaging system designed for visualization and analysis of microscopic samples. The system features a high-quality optical system and supports a range of imaging modalities, including brightfield, darkfield, and phase contrast microscopy. The DM1000 is intended to provide reliable and accurate microscopic imaging capabilities for various applications, such as material science, life science research, and quality control.
Automatically generated - may contain errors
4 protocols using dm1000 microimaging system
1
Histological Analysis of Tissue Samples
2
Histological Analysis of Chicken Intestine
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Histological analysis was performed adhering to the guidelines described in the previous study (Uni et al., 2001 (link)). Trimmed samples were dehydrated, cleared, and then embedded in paraffin. The samples were sectioned into 5 μm slices using a RM2235 microtome (Leica, Wetzlar, Germany), flattened onto glass slides, and then dried. After dewaxing with xylene, the sections were stained with hematoxylin and eosin (Thermo, Waltham, MA) and then sealed with neutral resin. Five sections of each of the chicken's intestinal specimen (duodenum, jejunum, and ileum) were taken, and pictures (400 × ) taken on them (5 each) were examined under a CX22 microscope (Olympus, Tokyo, Japan) with DM1000 microimaging system (Leica) randomly. The villus height as well as crypt depth were determined by Image Pro Plus 6.0. The villus/crypt ratio was calculated according to villus height/crypt depth.
3
Histological Analysis of Kidney Samples
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Histological analysis was performed adhering to the guidelines described in the previous study [11 (link)]. The kidney samples were fixed in 4% (wt/vol) buffered paraformaldehyde for 24 h. The trimmed samples were dehydrated, cleared and then embedded in paraffin. The samples were sectioned into 5 μm slices by using a RM2235 microtome (Leica, Munich, Germany), flattened onto glass slides and then dried. After dewaxing with xylene, the sections were stained with hematoxylin and eosin (Thermo, Waltham, MA, USA) and then sealed with neutral resin. The histopathological change of the kidneys was visualized under a CX22 microscope (Olympus, Tokyo Metropolitan, Japan), and a DM1000 microimaging system (Leica, Munich, Germany) was used to capture images.
4
Histological Analysis of Liver Samples
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Liver samples were fixed in 4% (wt/vol) buffered paraformaldehyde for 24 h. Trimmed samples were dehydrated in embedding cassettes (75% ethanol for 6 h, 85% ethanol for 10 h, 95% ethanol for 4 h, ethyl alcohol I for 2 h, and ethyl alcohol II for 2 h), cleared (xylene I for 20 min and xylene II for 15 min), immersed in paraffin for 3 h, and finally embedded. The samples were cut into 5 µm slices by using a RM2235 microtome (Leica, Germany), flattened onto glass slides in warm water, and then baked at 60 °C for at least 2 h. After dewaxing with xylene, the sections were flushed in water for 20 min, stained with hematoxylin (Thermo, USA) for 30 min, flushed again, differentiated with hydrochloric ethanol, and finally stained with eosin (Thermo, USA) for 5 min. After the sample was dehydrated and cleared again, the samples were sealed with neutral resin size. Liver steatosis and necrosis were observed under a CX22 microscope (Olympus, Japan), and DM1000 microimaging system (Leica, Germany) was used to capture images.
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