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Dcd flag construct orf clone ohu30172

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The DCD-FLAG construct (ORF clone OHu30172) is a laboratory tool used for protein expression and purification. It contains a coding sequence (open reading frame) that can be expressed in various host systems to produce recombinant proteins with a FLAG epitope tag. The FLAG tag allows for easy detection and affinity purification of the expressed proteins.

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Lab products found in correlation

Xav939 and rock inhibitor y 27632, by Merck Group (2 mentions) Grp78 n20 sc 1050 and dcd n20 sc 27465 antibodies, by Santa Cruz Biotechnology (2 mentions) Goat igg controls sc 2028, by Santa Cruz Biotechnology (2 mentions) Skbr3, by American Type Culture Collection (2 mentions) Imr 90, by American Type Culture Collection (2 mentions) Mda mb 231, by American Type Culture Collection (2 mentions) Mcf 7, by American Type Culture Collection (2 mentions) Phrl sv40, by Promega (2 mentions) Chir99021, by STEMCELL (2 mentions)

2 protocols using dcd flag construct orf clone ohu30172

1

Cell Line Generation and Culture Protocol

Cited 3 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
MCF7, MDA-MB-231, SK-BR-3 and IMR90 cells were purchased from American Type Culture Collection (ATCC). MDA-MB-231-GRP78ind cells [8 (link)] (Supplementary Figure 7), and human iPSC lines [26 (link), 27 (link)] were generated as previously reported. All cells were cultured as described previously [8 (link)]. DCD-FLAG construct (ORF clone OHu30172) was purchased from Genscript. M50 Super 8x TOPFlash and M51 Super 8x FOPFlash was a gift from Randall Moon (Addgene plasmid #12456). pHRL-SV40 was obtained from Promega. CHIR99021 was purchased from StemCell Technologies (#72054). XAV939 and ROCK inhibitor Y-27632 were purchased from Sigma (#X3004 and #SCM075). GRP78-N20 (#sc-1050) and DCD-N20 (#sc-27465) antibodies, or Goat-IgG controls (#sc-2028), used in inhibitory experiments, were purchased from Santa Cruz Biotechnology.
Remaining Materials and Methods are available as a Supplementary File.
Lager T.W., Conner C., Keating C.R., Warshaw J.N, & Panopoulos A.D. (2021). Cell surface GRP78 and Dermcidin cooperate to regulate breast cancer cell migration through Wnt signaling. Oncogene, 40(23), 4050-4059.
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2

Cell Line Generation and Culture Protocol

Cited 3 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
MCF7, MDA-MB-231, SK-BR-3 and IMR90 cells were purchased from American Type Culture Collection (ATCC). MDA-MB-231-GRP78ind cells [8 (link)] (Supplementary Figure 7), and human iPSC lines [26 (link), 27 (link)] were generated as previously reported. All cells were cultured as described previously [8 (link)]. DCD-FLAG construct (ORF clone OHu30172) was purchased from Genscript. M50 Super 8x TOPFlash and M51 Super 8x FOPFlash was a gift from Randall Moon (Addgene plasmid #12456). pHRL-SV40 was obtained from Promega. CHIR99021 was purchased from StemCell Technologies (#72054). XAV939 and ROCK inhibitor Y-27632 were purchased from Sigma (#X3004 and #SCM075). GRP78-N20 (#sc-1050) and DCD-N20 (#sc-27465) antibodies, or Goat-IgG controls (#sc-2028), used in inhibitory experiments, were purchased from Santa Cruz Biotechnology.
Remaining Materials and Methods are available as a Supplementary File.
Lager T.W., Conner C., Keating C.R., Warshaw J.N, & Panopoulos A.D. (2021). Cell surface GRP78 and Dermcidin cooperate to regulate breast cancer cell migration through Wnt signaling. Oncogene, 40(23), 4050-4059.
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