Model pointprobe

Manufactured by NanoWorld

Sourced in Switzerland

The Model Pointprobe is a highly specialized scanning probe microscope (SPM) designed for high-precision surface analysis. It features a sharp, nanometer-scale probe tip that scans across a sample surface, providing detailed topographical information and atomic-scale resolution. The core function of the Model Pointprobe is to capture accurate surface measurements and images without interpretation or extrapolation.

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Lab products found in correlation

Molecular imaging picoplus 2500 afm, by Agilent Technologies (2 mentions) Picoplus 2500 afm, by Agilent Technologies (2 mentions)

5 protocols using model pointprobe

Characterizing Surfactant-DNA Complexes by AFM

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The structures of the surfactant/ctDNA complexes were investigated using AFM. A Molecular Imaging PicoPlus 2500 AFM (Agilent Technologies, Santa Clara, CA, USA) was used. A resonance frequency of around 240 KHz and a nominal force constant of 42 N/m were the working conditions, using silicon cantilevers (Model Pointprobe, Nanoworld, Neufchâtel, Switzerland). The images were recorded in air and in tapping mode. Data collection was registered at 256 × 256 pixels, with scan speeds about 0.5 Hz. The ctDNA concentration was 0.6 μM, keeping pH = 7.4 (HEPES 10 mM).
Images of surfactant/ctDNA buffered solutions were obtained using the following method. (i) 0.1% (v/v) APTES aqueous solution was dropped onto a freshly cleaved mica surface in order to prepare a modified mica surface. After 20 min, it was washed with ultra-pure water and air dried. (ii) A 30 μL droplet of the surfactant/ctDNA buffered solution was deposited on the modified mica surface and incubated for 30 min. (iii) Afterwards, it was washed with pure water and air dried for AFM imaging.

Lebrón J.A., López-Cornejo P., García-Dionisio E., Huertas P., García-Calderón M., Moyá M.L., Ostos F.J, & López-López M. (2021). Cationic Single-Chained Surfactants with a Functional Group at the End of the Hydrophobic Tail DNA Compacting Efficiency. Pharmaceutics, 13(4), 589.

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AFM Imaging of AuNP-Oxalate Complexes

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Images were obtained with a Molecular Imaging PicoPlus 2500 AFM (Agilent Technologies). Silicon cantilevers (Model Pointprobe, Nanoworld) with a resonance frequency of around 240 kHz and nominal force constant of 42 N m⁻¹ were used. All AFM images were recorded in air and in tapping mode, with scan speeds of about 0.5 Hz and data collection at 256 × 256 pixels. At least two mica specimens were prepared for each solution, and at least 6 distinct areas of each specimen were investigated using the sample probe tip. The solutions were prepared in the absence and presence of 2.0 × 10⁻⁴ M [Ru(bpy)₃]²⁺ complex and 2.0 × 10⁻³ M C₂O₄²⁻ plus cacodylate buffer at pH 7.0 plus the suitable [AuNP].
100 μL of solutions was dropped onto a mica surface and adsorbed for 30 min in a humidified chamber. Following incubation, samples were washed with ultra-pure water and subsequently air dried for AFM imaging. In order to prevent the formation of salt crystals on the mica surface, the rinse was performed without cacodylate buffer.

Perez-Tejeda P., Martínez-Delgado A., Grueso E, & Giráldez-Pérez R.M. (2020). Measuring nanoparticle-induced resonance energy transfer effect by electrogenerated chemiluminescent reactions. RSC Advances, 10(7), 3861-3871.

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Lipoplex Structure Analysis by AFM

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Atomic force microscopy was used to study the structures of the lipoplexes. A resonance frequency of around 240 KHz and a nominal force constant of 42 N/m were the working conditions in a Molecular Imaging PicoPlus 2500 AFM (Agilent Technologies, Santa Clara, CA, USA). Silicon cantilevers (Model Pointprobe, Nanoworld, Neufchâtel, Switzerland) were used. The images were recorded in air and in tapping mode. Data collection (256 × 256 pixels) was registered with scan speeds about 0.5 Hz. The ctDNA concentration in the buffered HEPES 10 mM liposome solutions, pH = 7.4, was 0.6 μM.
Images of the buffered liposome solutions were obtained using the following method: (a) In order to prepare a modified mica surface, 0.1% (v/v) APTES aqueous solution was dropped onto a freshly cleaved mica surface. It was washed with ultra-pure water after 20 min and air dried. (b) A 30 μL droplet of the lipoplex solution was deposited on the modified mica surface and incubated for 30 min. (c) Subsequently, the mica surface was washed with pure water and air dried for AFM imaging.

Lebrón J.A., López-López M., García-Calderón C.B., V. Rosado I., Balestra F.R., Huertas P., Rodik R.V., Kalchenko V.I., Bernal E., Moyá M.L., López-Cornejo P, & Ostos F.J. (2021). Multivalent Calixarene-Based Liposomes as Platforms for Gene and Drug Delivery. Pharmaceutics, 13(8), 1250.

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Atomic Force Microscopy of Lipoplex Complexes

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Atomic force microscopy is a technique used to obtain information about the structure of lipoplex complexes. AFM images were obtained with a Molecular Imaging PicoPlus 2500 AFM (Agilent Technologies, Las Rozas, Madrid). Silicon cantilevers (Model Pointprobe, Nanoworld) with a resonance frequency of around 240 KHz and nominal force constant 42 N/m were used. All images were recorded in the tapping mode, with scan speeds of about 0.5 Hz and data collection at 256 × 256 pixels.
DNA dilute solutions (1.5 μmol dm⁻³) were used due to the large size of the polynucleotide. Thirty microliter of lipoplex (or free DNA) solution was deposited onto modified mica, incubated for 30 min, washed with pure water and air-dried for AFM imaging. The modified mica surface was prepared by dropping a 0.1% (v/v) APTES aqueous solution onto a freshly cleaved mica surface. After 20 min, the surface was washed with ultra-pure water and air dried [41 (link)].

Lebrón J.A., Ostos F.J., López-López M., Moyá M.L., Sales C., García E., García-Calderón C.B., García-Calderón M., Peña-Gómez M.J., Rosado I.V., R. Balestra F., Huertas P, & López-Cornejo P. (2020). Metallo-Liposomes of Ruthenium Used as Promising Vectors of Genetic Material. Pharmaceutics, 12(5), 482.

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Visualizing Calixarene-DNA Complexes via AFM

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The images were obtained with a Molecular Imaging PicoPlus 2500 AFM (Agilent Technologies). Silicon cantilevers (Model Pointprobe, Nanoworld) with a resonance frequency of around 240 KHz and nominal force constant of 42 N/m were used. All AFM imaging was recorded in air and in the tapping mode, with scan speeds of about 0.5 Hz, with data collection at 256 x 256 pixels.
In order to image isolated DNA molecules, it was necessary to use dilute solutions (0.3 mol dm -3 ) due to the large size of these molecules. AFM images of the calixarene/DNA complexes were obtained by drying a 30 L droplet of calixarene and DNA solution previously deposited on modified mica. The modified mica surface was prepared dropping a 0.1% (v/v) APTES aqueous solution onto a freshly cleaved mica surface. After 20 minutes, the surface was washed with ultra-pure water and air dried. A total of 30 L of DNA (or calixarene/DNA) solution was dropped onto this modified surface, incubated for 30 min, washed with pure water and air dried for AFM imaging.

Ostos F.J., Lebron J.A., Moyá M.L., Deasy M, & López-Cornejo P. (2015). Binding of DNA by a dinitro-diester calix[4]arene: denaturation and condensation of DNA. Colloids and surfaces. B, Biointerfaces, 127.

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