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2 protocols using resorcinol

1

Antioxidant Assays Protocol

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Solvents (ethanol, acetone, benzene, methanol and HCl, etc.) and chemicals (DPPH (C18H12N5O6); potassium persulphate (K2S2O8), Folin-Ciocalteu (C10H5NaO5S) reagent (FCR); disodium hydrogen orthophosphate, trichloroacetic acid (C2HCl3O2), ferric chloride (FeCl3), potassium ferricyanide (C6N6FeK3), sodium carbonate (Na2CO3), ammonium molybdate, 2,2′-azino-bis[3-ethylbenzothiazoline-6-sulfonate] (ABTS), etc.) were of either HPLC or analytic grade (Sigma Aldrich, HiMedia, India). Standards (HPLC grade) used during different antioxidant assays were procured from Sigma Aldrich. HPLC grade standards (Resorcinol, catechol, vanillin, ferulic acid, quercetin, benzoic acid, ascorbic acid, catechin, gallic acid and p-Coumaric acid) were procured from HiMedia and Sigma-Aldrich, respectively.
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2

Enzyme Assay Protocol for Polyphenol Oxidase

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Guaiacol, 2, 2′-azino bis[3-ethylbenzthiazoline-6-sulfonate] (ABTS), syringaldazine (SGZ), sephadex G-150 and DEAE-cellulose were purchased from Sigma (USA). Other substrates viz. pyrogallol, α-naphthol, p-phenylenediamine (PPD), resorcinol, 2, 6-dimethoxyphenol (DMP), catechol, 3,4-dihydroxy-phenylalanine (L-DOPA) and tyrosine were purchased from Hi-media. Other chemicals used were of analytical grade.
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