Thiazolyl blue tetrazolium bromide

Manufactured by Solarbio

Sourced in United States, China

Thiazolyl blue tetrazolium bromide is a sensitive colorimetric assay reagent used in various cell-based assays to measure cell viability, proliferation, and cytotoxicity. It functions by detecting the reduction of the tetrazolium dye to a purple-colored formazan product, which can be quantified spectrophotometrically.

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Lab products found in correlation

Penicillin streptomycin, by Solarbio (2 mentions) Fetal bovine serum (fbs), by Sartorius (2 mentions) Phosphate buffered saline (pbs), by Solarbio (2 mentions) M199 medium, by Sartorius (2 mentions) Dulbecco s modi ed eagle medium nutrient mixture f 12 dmem f12 at 1 1, by Sartorius (2 mentions) Neutral protease, by Solarbio (2 mentions) Collagenase, by Solarbio (2 mentions) D6430, by Solarbio (2 mentions) Trypsin, by Corning (1 mentions) Ripa lysis buffer, by Solarbio (1 mentions) Conduritol b epoxide, by MedChemExpress (1 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Annexin 5 fitc detection kit, by Beyotime (1 mentions) Pik3ca, by ABclonal (1 mentions) Pcr primers, by Sangon (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Ebioscience annexin 5 fitc apoptosis detection kit, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Thiazolyl blue tetrazolium bromide (MTT) (12 citations) Thiazolyl blue (2 citations) Thiazolyl blue tetrazolium bromide (MTT) powder (2 citations)

5 protocols using thiazolyl blue tetrazolium bromide

Cytotoxicity Evaluation of Zearalenone Metabolites

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Z14G was purchased from Qiyun Biotechnology Co., Ltd. (Guangzhou, China), ZEN was purchased from Yuanye Biology Co., Ltd. (Shanghai, China), α-Zearalenol (α-ZEL) and β-Zearalenol (β-ZEL) were purchased from Pribolab (Qindao, China).
Dulbecco’s modified eagle medium (DMEM), penicillin/streptomycin (100×), fetal bovine serum (FBS), phosphate buffered saline (PBS), and trypsin were purchased from Corning (USA), and RIPA lysis buffer and thiazolyl blue tetrazolium bromide were purchased from Solarbio (Beijing, China).
β-Glucosidase (6 U/mg, Duoxi) was purchased from Jinsui Biotechnology Co., Ltd. (Beijing, China); Conduritol B epoxide was purchased from Med Chem Express (Shanghai, China).

Ruan H., Wang Y., Hou Y., Zhang J., Wu J., Zhang F., Sui M., Luo J, & Yang M. (2022). Zearalenone-14-Glucoside Is Hydrolyzed to Zearalenone by β-Glucosidase in Extracellular Matrix to Exert Intracellular Toxicity in KGN Cells. Toxins, 14(7), 458.

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Isolation and Culture of Hepatocytes

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Phosphate-buffered saline (PBS), 0.25% pancreatin with ethylenediaminetetraacetic acid (EDTA), collagenase (C8140, 246 U/mg), neutral protease (D6430, 0.5 U/mg), penicillin-streptomycin and thiazolyl blue tetrazolium bromide (MTT) were purchased from Beijing Solarbio Biotechnology Co., Ltd. Beijing, China. collagenase and protease were dissolved in PBS to make 3000 U/mL and 0.5 U/mL, respectively. Percoll separation solution was diluted with PBS to 50%. Dulbecco's Modi ed Eagle Medium/Nutrient Mixture F-12 (DMEM/F12 at 1/1), M199 medium and fetal bovine serum (FBS) were purchased from Biological Industries (Kibbutz Beit-Haemek, Israel). A atoxin B 1 was purchased from Sigma-Aldrich (St. Louis, MO, U.S.), dissolved in 50% methanol to make 8 μg/mL AFB 1 concentration as the stock solution, ltered with 0.22 μm membrane high-ow lter (Sartorius Stedim Biotech Gmbh, Goettingen, Germany), and stored at 4℃ for the following experiment.

Guo H., Chang J., Wang P., Yin Q., Liu C., Xu X., Dang X., Hu X., & Wang Q. (2021). Effects of compound probiotics and aflatoxin-degradation enzyme on alleviating aflatoxin-induced cytotoxicity in chicken embryo primary intestinal epithelium, liver and kidney cells.

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Isolation of Cells Using Enzymatic Digestion

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Phosphate-buffered saline (PBS), 0.25% pancreatin with ethylenediaminetetraacetic acid (EDTA), collagenase (C8140, 246 U/mg), neutral protease (D6430, 0.5 U/mg), penicillin-streptomycin and thiazolyl blue tetrazolium bromide (MTT) were purchased from Beijing Solarbio Biotechnology Co., Ltd. Beijing, China. collagenase and protease were dissolved in PBS to make 3000 U/mL and 0.5 U/mL, respectively. Percoll separation solution was diluted with PBS to 50%. Dulbecco's Modi ed Eagle Medium/Nutrient Mixture F-12 (DMEM/F12 at 1/1), M199 medium and fetal bovine serum (FBS) were purchased from Biological Industries (Kibbutz Beit-Haemek, Israel). A atoxin B 1 was purchased from Sigma-Aldrich (St.
Louis, MO, U.S.), dissolved in 50% methanol to make 8 µg/mL AFB 1 concentration as the stock solution, ltered with 0.22 µm membrane high-ow lter (Sartorius Stedim Biotech Gmbh, Goettingen, Germany) , and stored at 4℃ for the following experiment.

Guo H., Chang J., Wang P., Yin Q., Liu C., Xu X., Dang X., Hu X., & Wang Q. (2020). Effects of Compound Probiotics and Aflatoxin-Degradation Enzyme on Alleviating aflatoxin-induced cytotoxicity in chicken embryo primary intestinal epithelium, liver and kidney cells.

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CKI Cytotoxicity and Apoptosis Assay

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CKI was purchased from the Shanxi Zhendong Group. RPMI 1640 and Dulbecco’s modified Eagle’s medium (DMEM) were purchased from Gibco (USA). Thiazolyl blue tetrazolium bromide was purchased from Solarbio (China); Annexin V-FITC detection kit from Beyotime Biotechnology (China); fetal bovine serum from Gibco (USA); TRIzol reagent from Ambion (Carlsbad, CA, USA); and qPCR kit from TaKaRa (Japan). The PCR primers were purchased from Sangon Biotech Co., Ltd. (China). PIK3CA, SRC, NOS3 primary antibodies were purchased from Abclonal(Wuhan, China).

Ye Y., Zhang B., Liang Q., Wang D., Bai F., Li Y., Wei L., Li L., Huang H, & Tang Y. (2022). Exploring the pharmacological mechanism of compound kushen injection in the treatment of breast cancer using in vitro experiments: Coupling network pharmacology with GEO database. Frontiers in Oncology, 12, 946758.

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Evaluation of Eeq Anticancer Effects

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Docetaxel was purchased from Selleck Chemicals (Cat# RP56976). RPMI 1640 medium was purchased from Gibco (Cat# 8120018). Fetal bovine serum was purchased from Gemini (Cat# 900-108). Charcoal stripped FBS was purchased from Biological Industries (Cat# 04-201-1A). Thiazolyl blue tetrazolium bromide (Cat# M8180) and crystal violet stain solution (Cat# G1063) were purchased from Solarbio life sciences. Human apoptosis array kit was from R&D Systems (Cat# ARY009). NuPAGETM 10% Bis-Tris Gel (Cat# NP0302BOX), eBioscience™ Annexin V-FITC apoptosis detection kit (Cat# BMS500FI-300), FxCycle™ PI/RNase staining solution (Cat# F10797) and Vybrant cell-labeling solutions (V22885) were purchased from Invitrogen. All antibodies used in this study were purchased from Proteintech Group (CDK4, Cat# 66950-1-lg; CDK6, Cat# 66278-1-lg; Cyclin D1, Cat# 26939-1-AP; RB1, Cat# 10048-1-lg; E2F1, Cat# 66515-1-lg; Survivin, Cat# 66495-1-lg; p21, Cat# 10355-1-AP). Eeq was isolated and identified from the marine sponge-derived fungus Fusarium equiseti SCSIO 41019 by various chromatographic and comprehensive spectroscopic methods in our previous study (Chen et al., 2019 (link)), respectively. It was determined to have ≥95% purity by analytical HPLC.

Wang X., Luo X., Gan X., Chen C., Yang Z., Wen J., Fang W., Huang H., Gao C., Zhou X., Feng X, & Liu Y. (2022). Analysis of regulating activities of 5′-epiequisetin on proliferation, apoptosis, and migration of prostate cancer cells in vitro and in vivo. Frontiers in Pharmacology, 13, 920554.

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