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Mouse cytokine array c3

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The Mouse Cytokine Array C3 is a multiplex immunoassay that allows for the simultaneous detection and quantification of 62 different mouse cytokines, chemokines, and other related proteins in a single sample. The array uses a sandwich immunoassay-based detection method to provide a sensitive, quantitative, and high-throughput analysis of these analytes.

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Lab products found in correlation

Protein assay method, by Bio-Rad (1 mentions) Raybio analysis tool, by RayBiotech (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) Quantity one, by Bio-Rad (1 mentions)

4 protocols using mouse cytokine array c3

1

Cytokine Profile in Mouse Bladder

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Mice were euthanized 48 hours after intravesical instillation of PBS or BacPAK6. Bladders were harvested and weighed. Bladder homogenates were obtained by adding 1 ml of a tissue lysis buffer (Fermentas, Maryland, USA) with a protease inhibitor cocktail (Merck, Darmstadt, Germany) to 50 mg of tissue sample and homogenizing by sonication. Bladder homogenates were then centrifuged at 16,000 × g for 30 min at 4 °C and the supernatants collected. Protein concentrations of the supernatants were determined by the Biorad protein assay method (Biorad, California, USA). An aliquot of the supernatant containing 80 μg of total protein concentration was loaded onto the Mouse Cytokine Array C3 (Raybiotech, Norcross, GA) to measure expression levels of cytokines and chemokines. RayBio® Analysis Tool (Raybiotech) was used to correlate the average signal intensities to relative expression levels of cytokines.
Ang W.X., Zhao Y., Kwang T., Wu C., Chen C., Toh H.C., Mahendran R., Esuvaranathan K, & Wang S. (2016). Local Immune Stimulation by Intravesical Instillation of Baculovirus to Enable Bladder Cancer Therapy. Scientific Reports, 6, 27455.
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2

Multiplexed Cytokine Profiling in Mice

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Blood was collected from mice via the sub-mandibular route at the end of drug treatment in tubes with anti-coagulant. Tubes were spun at 8,000 rpm for 15 minutes at 4°C to pellet the red blood cells. Plasma was carefully removed and stored in a separate tube. Protein quantitation was performed using BCA assay and was used to normalize the amount of protein loaded on to each membrane from Mouse Cytokine Array C3 (RayBiotech, Norcross, GA). Each treatment group (vehicle, leflunomide and flutamide) had three biological replicates. The manufacturer’s protocol was followed for processing the membranes. The images were inverted to neutralize the effect of any differences in background, and intensity of the dots was measured using ImageJ. Since each antibody on the membrane was blotted in duplicate, the mean intensity of the dots was calculated, then averaged across the biological replicates and normalized to vehicle group.
Choudhary M., Safe S, & Malek G. (2018). Suppression of aberrant choroidal neovascularization through activation of the aryl hydrocarbon receptor. Biochimica et biophysica acta, 1864(5 Pt A), 1583-1595.
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3

Multiplex Cytokine Profiling in Mice

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Proteome Profiler Mouse Cytokine Array Kit, Panel A (R&D Systems, Minneapolis, MN) detecting 40 mouse cytokines was used to detect C5a and other cytokines in mouse serum samples pulled from three mice each. Array was used according to the manufacturer’s instructions. Mouse Cytokine Array C3 (RayBiotech, Inc., Norcross, GA) detecting 62 mouse cytokines and related proteins was used to analyze individual mouse lung extracts. Array was used according to the manufacturer’s instructions.
Schonfeld M., Villar M.T., Artigues A., Weinman S.A, & Tikhanovich I. (2021). Arginine Methylation of Hepatic hnRNP H Suppresses Complement Activation and Systemic Inflammation in Alcohol‐Fed Mice. Hepatology Communications, 5(5), 812-829.
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4

Anakinra Modulates Cytokine Profiles

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Gingival fibroblasts were serum-starved for 24 h, then treated with 1 μg/ml LPS with or without 500 ng/mg anakinra for 24 h. Cytokine concentrations in cell lysates were then determined using the Mouse Cytokine Array C3 (RayBiotech, Inc., GA) following manufacturer’s instructions. Data were quantitated with Quantity One (Bio-Rad Laboratories)
Cheng R., Billet S., Liu C., Haldar S., Choudhury D., Tripathi M., Hav M., Merchant A., Hu T., Huang H., Zhou H, & Bhowmick N.A. (2019). Periodontal inflammation recruits distant metastatic breast cancer cells by increasing myeloid-derived suppressor cells. Oncogene, 39(7), 1543-1556.
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