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3 protocols using chlorogenic acid

1

Characterization of Bioactive Compounds

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Caffeine (99% of purity), 1,1-diphenyl-2-picrilhhydrasyl and quercetin (95% of purity) were provided by Sigma-Aldrich (São Paulo, Brazil), methanol and aluminum chloride by Synth (São Paulo, Brazil), Folin–Ciocalteau, ethanol, gallic acid, algin, benzoic acid, glycerin and sodium hydroxide by Dinâmica (São Paulo, Brazil), acetonitrile by J.T. Baker (Phillipsburg, New Jersey, United States), acetic acid, sodium carbonate, tannic acid, lecithin, cetyl alcohol and sorbic acid by Êxodo Científica (Sumaré, São Paulo, Brazil), chlorogenic acid (95% of purity) by Fluorochem (Hadfield, United Kingdom) and persea gratissima (avocado) oil by Croda (Campinas, São Paulo, Brazil). All solvents and reagents used were of analytical grade.
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2

Quantification of Phenolic Compounds

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Gallic acid hydrate was from Panreac (Barcelona, Spain). Glycine (99.5%) was from Applichem (Darmstadt, Germany). Iron chloride hexahydrate was from Merck (Darmstadt, Germany). L-Lactic acid, ascorbic acid (99.5%), sodium acetate trihydrate, sodium carbonate anhydrous (99%) and aluminum chloride anhydrous (98%) were from Penta (Praha, Czechia). Chlorogenic acid (95%) was from Fluorochem (Hadfield, UK). NeoChlorogenic acid (≥98%) was from Merck (Darmstadt, Germany). Folin-Ciocalteu reagent, rutin (quercetin 3-O-rutinoside) hydrate (>94%), isorhamnetin 3-O-glucoside and 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ) were from Sigma-Aldrich (St. Louis, MO, USA).
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3

Phytochemical Compound Characterization

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NeoChlorogenic acid (≥98%) was from Merck (Darmstadt, Germany). Chlorogenic acid (95%) was from Fluorochem (Hadfield, UK). Ferulic acid, caffeic acid, Folin-Ciocalteu reagent, 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), hydroxypropyl β-cyclodextrin and 2,2-diphenylpicrylhydrazyl (DPPH) were from Sigma-Aldrich (Darmstadt, Germany). Sodium hydroxide and iron chloride hexahydrate was from Merck (Darmstadt, Germany). Sodium carbonate anhydrous (99%), aluminum chloride anhydrous (98%) and ethanol (99.8%) were from Penta (Praha, Czechia). All solvents used for chromatography were HPLC grade.
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