Hep 3b2

MCF7, MCF10A, A549, H1299, SHSY5Y, Hep G2, Hep 3B2, HT-1080, NCI-H358, LLC, Neuro-2a, 4T1 and SK-N-BE2c cells lines were obtained from the American Type Culture Collection and their identity was not further authenticated. These are not listed in the database of commonly misidentified cell lines maintained by ICLAC. LLC, Neuro-2a, 4T1, Hep G2, HT-1080, Hep 3B2, MCF7 and A549 cells were cultured at 37°C in Dulbecco’s modified Eagle medium (DMEM) with 10% fetal bovine serum (FBS), 5ml of 100 U/ml Penicillin-Streptomycin (Pen Strep, Life Technologies) and 5ml of L-Glutamine 200mM. NCI-H358, H1299 and SK-N-BE2c cell lines were cultured at 37°C in Roswell Park Memorial Institute (RPMI) 1640 Medium (RPMI) 10% FBS 1% Pen Strep and 1% L-Glutamine. MCF10A cells were cultured at 37°C in DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) supplemented with 5% horse serum (Life Technologies), 20 ng/ml human Epidermal Growth Factor (Prepotec), 0.5 μg/ml hydrocortisone, 100ng/ml cholera toxin, 10 μg/ml insulin, and 100 U/ml Pen Strep. The SHSY5Y cell line was cultured at 37°C in DMEM/F-12 supplemented with 10% FBS, 2% (PenStrep) and 1% Non Essential Amino Acids (MEM). Mouse J1 ES cells were cultured feeder-free in fibroblast-conditioned medium. Cell cultures were confirmed to be mycoplasma-free every month.