Cy3 conjugated antibodies

Enhanced chemiluminescence (ECL) reagents, nitrocellulose membranes (Hybond-C extra), and secondary Cy3-conjugated antibodies were from Amersham Pharmacia Biotech Inc. (Piscataway, NJ, USA). Polyclonal antibodies against NF-kB p65 (#8242), IKKβ (#2370) and p-IKK α/β (#2697) or monoclonal antibodies against IKB (#4814) and p-IKB (#9246) were from Cell Signalling Technologies (Danvers, Massachusetts); polyclonal antibodies against IL-1β (sc-7884), caspase p10 (sc-515) and LaminA (sc-20680) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA); monoclonal antibodies against NLRP3 were from Abcam (Cambridge, UK, code ab109314) or from Adipogen (Adipogen AG, Liestal, Switzerland, code AG-20B-0014-C100). Monoclonal antibodies for α-tubulin and β-actin and all other reagents of analytical grade were from Sigma Chemical Co (Sigma Aldrich Spa, Milan, Italy). HiPerfect Transfection Reagent was from Qiagen (QIAGEN S.r.l., Milan, Italy). ELISA kit for human IL-1β was from Abcam (Cambridge, UK, code ab100562).

Enhanced chemiluminescence (ECL) reagents, nitrocellulose membranes (Hybond-C extra), and secondary Cy3-conjugated antibodies were from Amersham Pharmacia Biotech Inc. (Piscataway, NJ, USA). Monoclonal antibody against SERPINB3 (sc-21767), LaminA (sc-20680) and PCNA (sc-25280) or polyclonal antibody for HIF-1α (sc-53546) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Polyclonal antibody for HIF-2α and HO-1 were from Novus Biologicals (Cambridge, UK) and Stressgen (Victoria, BC, Canada), respectively. Monoclonal antibodies for α-tubulin and β-actin and all other reagents of analytical grade were from Sigma Chemical Co (Sigma Aldrich Spa, Milan, Italy), Lipofectamine 2000 (Invitrogen-Life Technologies), Plasmid DNA purification NucleoBond XtraMIDI (Macherey-Nagel, Germany), pCMV6-Entry vectors (Origene, Rockville, MD), Boyden's chambers were from Neuro Probe, Inc. (MD, USA).