Rabbit anti h3k9ac

Manufactured by Merck Group

Rabbit anti-H3K9ac is an antibody that specifically recognizes the acetylation of lysine 9 on histone H3. This antibody can be used for various applications in molecular biology and epigenetics research, such as chromatin immunoprecipitation (ChIP) and western blotting.

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2 protocols using rabbit anti h3k9ac

Antibody Validation for Immunoblotting and ChIP

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The following antibodies were used for immunblot analysis: mouse anti-AcSmc3 (gift from K. Shirahige), rabbit anti-CTCF (Peters laboratory ID A992), goat anti-H3 (Santa Cruz sc-8654), mouse anti-Scc1 (Upstate 05-908), rabbit anti-Smc3 (Bethyl A300-060A), mouse anti-tubulin (Sigma B512) and rabbit anti-Wapl (Peters laboratory ID A960). The following antibodies were used for immunofluorescence microscopy: rabbit anti-CTCF (Peters laboratory ID A992) and mouse anti-Scc1 (Upstate 05-908). The following antibodies were used for ChIP experiments: rabbit anti-CTCF (Upstate 07-729), rabbit anti-Scc1 (Abcam AB992), rabbit anti-Stag1 (Peters laboratory ID A823), rabbit anti-Nipbl (Sanova A301-779A), rabbit anti-H3K4me2 (Millipore 07-030) and rabbit anti-H3K9ac (Millipore 07-352).

Busslinger G.A., Stocsits R.R., van der Lelij P., Axelsson E., Tedeschi A., Galjart N, & Peters J.M. (2017). Cohesin is positioned in mammalian genomes by transcription, CTCF and Wapl. Nature, 544(7651), 503-507.

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Western Blot and ChIP Antibody Characterization

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The antibodies for Western blot analysis used were: mouse anti-α-tubulin (1:10,000; Sigma, clone B512), rat anti-α-synuclein (1:100; clone 15G7), mouse anti-α-synuclein (1:1000; BD, Synuclein-1), rabbit anti-GFP (1:1000; Santa Cruz), rabbit anti-H3 (1:16000; abcam), rabbit anti-H3K4me3 (1:16000; Abcam), rabbit anti-H3K9me1 (1:16000; Abcam), rabbit anti-H3K9me2 (1:4000; CST), rabbit anti-H3K9me3 (1:16000; Abcam), rabbit anti-H3K27me3 (1:8000; Millipore), rabbit anti-H3K9ac (1:8000; Millipore), rabbit anti-H3K14ac (1:8000; EpiGentek), rabbit anti-H4 (1:16000; Abcam), rabbit anti-H4K12Ac (1:16000; Abcam), rabbit anti-HP1α (1:1000; CST), rabbit anti-EHMT2 (1:2000; abcam), rabbit anti-p38 (1:1000; CST), goat anti-REST (1:250; Santa Cruz) and goat anti-SNAP25 (1:1000; Santa Cruz). The HRP-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories (1:10,000). The antibodies for chromatin immunoprecipitation (ChIP) used were: rabbit anti-EHMT2 (10 μg; Millipore), mouse anti-H3K9me2 (4 μg; Abcam), and rabbit anti-REST (10 μg; Millipore).

Sugeno N., Jäckel S., Voigt A., Wassouf Z., Schulze-Hentrich J, & Kahle P.J. (2016). α-Synuclein enhances histone H3 lysine-9 dimethylation and H3K9me2-dependent transcriptional responses. Scientific Reports, 6, 36328.

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