Humanomniexpress 12 v1.1 beadchip arrays

Manufactured by Illumina

Sourced in United States

The HumanOmniExpress-12 v1.1 BeadChip arrays are a high-throughput genotyping platform designed to interrogate over 950,000 genetic markers across the human genome. These arrays provide a comprehensive coverage of common and rare genetic variation for genome-wide association studies and other genetic research applications.

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Lab products found in correlation

Infinium hd assay ultra protocol, by Illumina (1 mentions) Iscan system, by Illumina (1 mentions)

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HumanOmniExpress BeadChip (148 citations) HumanOmniExpress (97 citations) BeadChips (68 citations) HumanOmniExpress array (32 citations) HumanOmniExpress‐12 BeadChip (8 citations) HumanOmniExpress BeadChip array (6 citations) HumanOmniExpress v1 (6 citations) BeadChip arrays (6 citations) HumanOmniExpress-12 (4 citations) HumanOmniExpress-12 array (2 citations)

2 protocols using humanomniexpress 12 v1.1 beadchip arrays

Eif2s1 Genotyping and fMRI Analysis

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DNA was isolated from buccal swabs and genotyping was completed using Illumina (San Diego, CA) HumanOmniExpress-12 v1.1 BeadChip arrays (cat# WG-312-1120) containing approximately 741K SNPs. Genomic DNA (200 ng) from each sample was processed following Illumina’s Infinium HD Ultra Assay protocol. BeadChip images were captured using Illumina iScan System. The Illumina chip contained the Eif2s1 rs10144417 SNP (Global minor allele frequency MAF:0.2993). fMRI activity was studied according to the genotype of analyzed subjects. Subjects with AG and GG genotypes were pooled for analysis and compared to subjects with the AA genotype using AFNI’s statistical software.

Placzek A.N., Molfese D.L., Khatiwada S., Viana Di Prisco G., Huang W., Sidrauski C., Krnjević K., Amos C.L., Ray R., Dani J.A., Walter P., Salas R, & Costa-Mattioli M. (2016). Translational control of nicotine-evoked synaptic potentiation in mice and neuronal responses in human smokers by eIF2α. eLife, 5, e12056.

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Genome-Wide Genotyping and Imputation for Dunedin Cohort

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We used Illumina HumanOmni Express 12v1.1 BeadChip arrays (Illumina CA, USA) to assay common Single Nucleotide Polymorphism (SNP) variation in the genomes of our cohort members. We imputed additional SNPs using the impute2 software (version 2.3.1, https://mathgen.stats.ox.ac.uk/impute/impute_v2.html) and 1000 Genomes version-3 reference panel. Imputation was conducted on autosomal SNPs appearing in dbSNP (v140) that were called in >98% of the Dunedin Study samples. Invariant SNPs were excluded. Pre-phasing and imputation were conducted using a 50M base-pair sliding window. The resulting genotype database included genotyped SNPs and SNPs imputed with 90% probability of a specific genotype among the non-Maori members of the Dunedin cohort (n=918) and in Hardy-Weinberg equilibrium (p>0.01 for all).

Belsky D.W., Moffitt T.E., Corcoran D.L., Domingue B., Harrington H., Hogan S., Houts R., Ramrakha S., Sugden K., Williams B.S., Poulton R, & Caspi A. (2016). The Genetics of Success: How Single-Nucleotide Polymorphisms Associated With Educational Attainment Relate to Life-Course Development. Psychological Science, 27(7), 957-972.

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