The 10 μg/mL LPS was used to treat IEC-6 and Caco-2 cells (National Infrastructure of Cell Line Resource, Beijing, China) for 24 h to establish intestinal epithelial cell injury models. After obtaining the LPS-injured intestinal epithelial cell model, 1 × 106 BMMSCs were added to the upper layer of a 0.8-μm Transwell chamber (Corning Inc., Corning, NY, USA), and the lower layer comprised intestinal epithelial cells. The cells in the chambers were co-cultured for 24 h, to rule out the role of MSCs in direct contact with intestinal epithelial cells. The activity of intestinal epithelial cells and the levels of tight junction proteins were determined. According to the different treatment methods of intestinal epithelial cells, they were divided into the same for groups as the injury model of steatotic IAR20 cells.
0.8 μm transwell chamber
Manufactured by Corning
Sourced in United States
The 0.8-μm Transwell chamber is a laboratory equipment used for in vitro cell culture applications. It features a polycarbonate membrane with a pore size of 0.8 micrometers, which allows for the study of cellular interactions and barrier functions.
Automatically generated - may contain errors
2 protocols using 0.8 μm transwell chamber
1
Intestinal Epithelial Cell Injury Model
2
Immunohistochemical Analysis of SOX10
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The immunohistochemical antibody for SOX10 was procured from Fuzhou Maixin Biotechnology Development Co., Ltd. Other reagents such as TRIzol, Mir-X miRNA First-Strand Synthesis, and SYBR Green PCR kits were purchased from TAKARA in Japan. A 0.8 μm Transwell chamber was sourced from Corning (USA), and Matrigel was obtained from BD (USA). Monoclonal antibodies specific to E-cadherin, N-cadherin, and Vimentin were purchased from Wuhan Sanying Company in China. Fetal bovine serum (FBS) was acquired from Israel BI Company, and DMEM medium was supplied by Gibco (USA).
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