Oligonucleotides used for the preparation of radiolabeled substrates and ssDNA competitor were synthesized and gel‐purified by Bioneer (Daejeon, Korea). [γ‐32P]ATP (3000 Ci·mmol−1) was purchased from Perkin Elmer (Waltham, MA, USA). PCR primers were synthesized by Cosmo Genetech (Seoul, Korea). Subtilisin, MMS, CPT, propidium iodide, and poly(dI‐dC) were purchased from Sigma‐Aldrich (St. Louis, MO, USA). M13mp18 sscDNA was acquired from New England Biolabs (Ipswich, MA, USA). α‐factor was purchased from GenScript (Piscataway, NJ, USA). Restriction enzymes, T4 polynucleotide kinase, and protein size markers were obtained from Enzynomics (Daejeon, Korea).
Pcr primers
Manufactured by Cosmogenetech
PCR primers are short DNA sequences used in the Polymerase Chain Reaction (PCR) process to selectively amplify target DNA sequences. They serve as the starting point for DNA replication by binding to specific regions on the DNA template, allowing the DNA polymerase enzyme to replicate the target sequence.
Automatically generated - may contain errors
Lab products found in correlation
Subtilisin, by Merck Group (1 mentions)
Poly di dc, by Merck Group (1 mentions)
T4 polynucleotide kinase, by Enzynomics (1 mentions)
Restriction enzymes, by Enzynomics (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
γ 32p atp, by PerkinElmer (1 mentions)
α factor, by GenScript (1 mentions)
Topreal qpcr 2 premix sybr green with low rox, by Enzynomics (1 mentions)
Cfx manager, by Bio-Rad (1 mentions)
Taq polymerase, by Thermo Fisher Scientific (1 mentions)
Improm 2 reverse transcriptase kit, by Promega (1 mentions)
Cfx96 real time system, by Bio-Rad (1 mentions)
2 protocols using pcr primers
1
Preparation of Radiolabeled Substrates and ssDNA
2
Quantitative RT-PCR Analysis of Mouse mRNA
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RNA was isolated using the ImProm-II Reverse Transcriptase kit (Promega) and cDNA was synthesized using oligo dT. PCR primers were commercially synthesized (Cosmo Genetech). qRT-PCR was performed on the reverse transcribed product using Taq Polymerase (Invitrogen) and primers (Supplementary Table 1 ) specific for mouse cDNAs. TOPrealTM qPCR 2 × PreMIX (SYBR Green with low ROX) (Enzynomics) was used. Amplification (50 cycles) was performed in a CFX96 Real-Time System (Bio-Rad). Actb or 18S rRNA was used as the reference genes for normalization. CFX-manager (Bio-Rad) was used for analysis. The primers used are listed in Supplementary Table 1 .
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