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Thepretrox tight pur luc plasmid

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The pRetroX-Tight-Pur-Luc plasmid is a lentiviral expression vector that contains a tetracycline-inducible promoter and a luciferase reporter gene. It is designed for the regulated expression of luciferase in mammalian cell lines.

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Lab products found in correlation

Pgl4.10 vector, by Promega (2 mentions)

2 protocols using thepretrox tight pur luc plasmid

1

Cloning and Characterizing AR Promoter Regions

Check if the same lab product or an alternative is used in the 5 most similar protocols
AR promoter regions P1 (+1.1kb-1.7kb), P2 (1.7kb-2.5kb), P1+P2
(+1.1kb-2.5kb) and AR promoter fragments (0.4–0.8kb,
0.8–1.2kb, 1.2–1.6kb, 1.6–1.8kb, 1.8–2.3kb) were
amplified by PCR from LNCaP genomic DNA. The AR promoter P1, P2 and full
length were inserted into pGL4.10 vector (catalog number E6651; Promega) by
using XhoI and HindIII sites and AR fragments were cloned into the
pRetroX-Tight-Pur-Luc plasmid (Clontech laboratories, Inc.) by using BamHI
and BglII. All plasmids were verified by sequencing.
Kim J., Lee Y., Lu X., Song B., Fong K.W., Cao Q., Licht J.D., Zhao J.C, & Yu J. (2018). Polycomb- and Methylation-Independent Roles of EZH2 as a Transcription Activator. Cell reports, 25(10), 2808-2820.e4.
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2

Cloning and Characterizing AR Promoter Regions

Check if the same lab product or an alternative is used in the 5 most similar protocols
AR promoter regions P1 (+1.1kb-1.7kb), P2 (1.7kb-2.5kb), P1+P2
(+1.1kb-2.5kb) and AR promoter fragments (0.4–0.8kb,
0.8–1.2kb, 1.2–1.6kb, 1.6–1.8kb, 1.8–2.3kb) were
amplified by PCR from LNCaP genomic DNA. The AR promoter P1, P2 and full
length were inserted into pGL4.10 vector (catalog number E6651; Promega) by
using XhoI and HindIII sites and AR fragments were cloned into the
pRetroX-Tight-Pur-Luc plasmid (Clontech laboratories, Inc.) by using BamHI
and BglII. All plasmids were verified by sequencing.
Kim J., Lee Y., Lu X., Song B., Fong K.W., Cao Q., Licht J.D., Zhao J.C, & Yu J. (2018). Polycomb- and Methylation-Independent Roles of EZH2 as a Transcription Activator. Cell reports, 25(10), 2808-2820.e4.
+ Open protocol
+ Expand

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