Aperio at system

Manufactured by Leica

Sourced in Germany

The Aperio AT system is a digital pathology imaging solution that captures high-resolution images of tissue samples. It enables automated slide scanning and image management for diagnostic and research applications.

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Lab products found in correlation

Bond polymer refine red detection kit, by Leica (1 mentions) Mouse monoclonal anti cd8 antibody clone c8 144b, by Agilent Technologies (1 mentions) Dab chromogen kit, by Biocare Medical (1 mentions) Imagescope software, by Leica (1 mentions) Autostainer, by Agilent Technologies (1 mentions)

Spelling variants of this product

Aperio System (18 citations) Aperio Scanscope System AT (2 citations) Aperio ScanScope AT Turbo system (2 citations)

3 protocols using aperio at system

Quantifying Immune Cell Infiltration in Melanoma

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Single-marker IHC was performed on formalin-fixed paraffin-embedded (FFPE) tumor sample sections, (4 μm thickness) after heat-induced antigen retrieval (HIER, citrate buffer, pH 6.0), obtained from patients with melanoma at the designated treatment timepoints. Granzyme B was localized by immuno-labeling tissue sections with mouse mAb (clone GrB-7; DAKO) at 1:25 dilution followed by detection with the EnVisionTM G2 System/AP kit and visualization by Permanent Red Chromogen substrate staining. CD8 was labeled in FFPE tissue sections using mouse monoclonal anti-CD8 antibody (clone C8/144B; DAKO) at a 1:100 dilution followed by detection with the BOND Polymer Refine Red Detection Kit (Leica) and AP Red chromogen substrate visualization. Whole-slide scans were imaged using the Aperio-AT system (Leica Biosystems) and transferred into the HALO (Indica Labs) platform for quantitative digital image analysis.

Andtbacka R.H., Wang Y., Pierce R.H., Campbell J.S., Yushak M., Milhem M., Ross M., Niland K., Arbeit R.D., Parasuraman S., Bickley K., Yeung C.C., Aicher L.D., Smythe K.S, & Gan L. (2022). Mavorixafor, an Orally Bioavailable CXCR4 Antagonist, Increases Immune Cell Infiltration and Inflammatory Status of Tumor Microenvironment in Patients with Melanoma. Cancer Research Communications, 2(8), 904-913.

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Quantitative Aortic Collagen and pSMAD2/3

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Slides that were stained for collagen and pSMAD2/3 were digitized on an Aperio AT system (Leica Biosystems, Nussloch, Germany). Using Aperio ImageScope tools, the area of staining were measured. That area was divided by the total area of the vascular layer or the vascular media in the same aortic ring section.

Hori D., Dunkerly-Eyring B., Nomura Y., Biswas D., Steppan J., Henao-Mejia J., Adachi H., Santhanam L., Berkowitz D.E., Steenbergen C., Flavell R.A, & Das S. (2017). miR-181b regulates vascular stiffness age dependently in part by regulating TGF-β signaling. PLoS ONE, 12(3), e0174108.

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Multiplex Immunohistochemistry for Tumor Profiling

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Immunohistochemistry (IHC) for CD8, CD39, PD1 and CD19 staining was performed on mouse tumors harvested at the end point (day 23), in 10% neutral buffered formalin (NBF). Tumor sections were cut at 3 μm onto superfrost+ glass slides and stored under vacuum until IHC was performed. Antibody specific IHC conditions are listed in Table S2. Briefly, antigen retrieval was done using a pressure decloaker (100°C; 20 minutes) and IHC was performed on a Dako Autostainer. Targets were visualized using the MACH3 HRP polymer detection system (Biocare; M3R531) and DAB Chromogen Kit (Biocare; BDB2004). Slides were counterstained with diluted hematoxylin and scanned using the 20x object lens on an Aperio AT system (Leica Biosystems). Representative images per tumor were captured using ImageScope software (Leica Biosystems).

Li X.Y., Moesta A.K., Xiao C., Nakamura K., Casey M., Zhang H., Madore J., Lepletier A., Aguilera A.R., Sundarrajan A., Jacoberger-Foissac C., Wong C., Cruz T.D., Welch M., Lerner A.G., Spatola B.N., Soros V.B., Corbin J., Anderson A.C., Effern M., Hölzel M., Robson S.C., Johnston R.L., Waddell N., Smith C., Bald T., Geetha N., Beers C., Teng M.W, & Smyth M.J. (2019). Targeting CD39 in cancer reveals an extracellular ATP and inflammasome driven tumor immunity. Cancer discovery, 9(12), 1754-1773.

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