Il 1β elisa kit

Ang II was purchased from APExBIO Technology LLC, whilst pitavastatin was purchased from HL Genomics, Co., Ltd. Antibodies were purchased as follows: Anti-phosphorylated (p)-endothelial NO synthase (eNOS; cat. no. ab215717; 1:1,000; Abcam), anti-eNOS (cat. no. ab76198; 1:1,000; Abcam), anti-endothelin (ET)1 (cat. no. ab2786; 1:1,000; Abcam), anti-Bcl-2 (cat. no. ab32124; 1:1,000; Abcam), anti-Bax (cat. no. ab32503; 1:1,000; Abcam), anti-cleaved caspase-3 (cat. no. ab2302; 1:1,000; Abcam), anti-cleaved poly (ADP-ribose) polymerase 1 (PARP1; cat. no. ab32064; 1:1,000; Abcam), anti-ERK5 (cat. no. ab40809; 1:1,000; Abcam), anti-GAPDH (cat. no. ab181602; 1:2,000; Abcam), HRP-conjugated goat anti-mouse IgG H&L (cat. no. ab205719; 1:10,000; Abcam), HRP-conjugated goat anti-rabbit IgG H&L (cat. no. ab205718; 1:10,000; Abcam) and Alexa Fluor^® 488 goat anti-rabbit IgG H&L (cat. no. ab150077; 1:10,000; Abcam).
The following kits were purchased as follows: NO Colorimetric assay kit (cat. no. E-BC-K035-S; Elabscience Biotechnology, Inc.), reactive oxygen species (ROS) colorimetric assay kit (cat. no. E-BC-K138-F; Elabscience Biotechnology, Inc.), TNF-α ELISA kit (cat. no. ADI-901-099; Enzo Life Sciences, Inc.), IL-1β ELISA kit (cat. no. EHC002b.48; NeoBioscience Technology Co., Ltd.) and the IL-6 ELISA kit (cat. no. ADI-901-033; Enzo Life Sciences, Inc.).

N2A cells were induced with 10 μM of Aβ_1-42 and 2 mM of l-Glutamic acid (Echo Chemical Co. Ltd) for 24 h, respectively. BV2 and RAW264.7 cells were induced with 20 μl (2 μg/ml) LPS for 3 h, and treated with d.d.H₂O, atorvastatin (10 μM), <1 kDa, 1–3 kDa, 3–10 kDa and >10 kDa Chlorella Pyrenoidosa peptides (10 μg/mL) for 24 h. The cellular cytokines levels in the cell culture medium were measured using mouse PGE2, IL-6, iNOS, TNF-α, COX-2, IL-1β ELISA Kit (Enzo Biochem, USA), according to the enzyme-linked immunosorbent assay manufacturer's protocol.