100cxii transmission electron microscope

Manufactured by Ametek

The 100CXII Transmission Electron Microscope is a laboratory instrument designed for high-resolution imaging and analysis of microscopic samples. It utilizes a beam of electrons to produce magnified images of specimens, enabling detailed examination of their structure and composition at the nanoscale level.

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Lab products found in correlation

Orius digital camera, by Ametek (2 mentions)

2 protocols using 100cxii transmission electron microscope

Negative Staining of Extracellular Vesicles

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Sample was prepared as previously reported with slight modifications [33 ]. Isolated EVs were fixed in 2% paraformaldehyde (PFA) for 5 min. For negative-staining of EVs, 5μl of the sample solution was placed on a carbon coated EM grid and EVs were immobilized for 1 min. The grid was transferred to five 100 μl drops of distilled water and letting it for 2 min on each drop. The sample was negative-stained with 1% uranyl acetate. The excess uranyl acetate was removed by contacting the grid edge with filter paper and the grid was air-dried. The grids were imaged with a JEOL 100CXII Transmission Electron Microscope operating at 100 kV. Images were captured on a Gatan Orius Digital Camera.

Kanada M., Kim B.D., Hardy J.W., Ronald J.A., Bachmann M.H., Bernard M.P., Perez G.I., Zarea A.A., Ge T.J., Withrow A., Ibrahim S.A., Toomajian V., Gambhir S.S., Paulmurugan R, & Contag C.H. (2019). Microvesicle-mediated delivery of minicircle DNA results in effective gene-directed enzyme prodrug cancer therapy. Molecular cancer therapeutics, 18(12), 2331-2342.

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Negative-Staining of Extracellular Vesicles

Check if the same lab product or an alternative is used in the 5 most similar protocols

Isolated EVs were fixed in 2% paraformaldehyde (PFA) for 5 min. For negative-staining of EVs,

5 μ l

of the sample solution was placed on a carbon-coated EM grid and EVs were immobilized for 1 min. Next, the grid was transferred to five

100 μ l

drops of distilled water and letting it for 2 min on each drop. The sample was negative-stained with 1% uranyl acetate. The excess uranyl acetate was removed by contacting the grid edge with filter paper and the grid was air-dried. The grids were imaged with a JEOL 100CXII Transmission Electron Microscope operating at 100 kV. Images were captured on a Gatan Orius Digital Camera.

Mias G.I., Singh V.V., Rogers L.R., Xue S., Zheng M., Domanskyi S., Kanada M., Piermarocchi C, & He J. (2021). Longitudinal saliva omics responses to immune perturbation: a case study. Scientific Reports, 11, 710.

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