Et1609 42

Manufactured by Huabio

The ET1609-42 is a piece of lab equipment designed for precise measurements. It features a high-resolution display and intuitive controls for easy operation. The core function of this product is to provide accurate and reliable data collection for research and laboratory applications.

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Lab products found in correlation

Polyethylenimine (pei), by Merck Group (1 mentions) Anti ha, by Merck Group (1 mentions) Anti ha, by Roche (1 mentions) Jetprime, by Polyplus Transfection (1 mentions) S7719, by Selleck Chemicals (1 mentions) Anti β tubulin, by Transgene (1 mentions) Hrp conjugated affinipure goat anti rabbit igg h l, by Proteintech (1 mentions) Anti gapdh, by Proteintech (1 mentions) Enhanced chemiluminescence reagent, by Thermo Fisher Scientific (1 mentions) Sc17753, by Santa Cruz Biotechnology (1 mentions) Et1603 11, by Huabio (1 mentions) Et1603 34, by Huabio (1 mentions) Et1701 54, by Huabio (1 mentions)

2 protocols using et1609 42

Optimized Cell Transfection and Signaling Pathway Inhibition

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Polyethylenimine (PEI) (764582, Sigma-Aldrich) and jetPRIME (114-15, Polyplus) were used for transfection. Quenched fluorescent reporter RNA was purchased from General Biology. Inhibitors used in this study include the following: p38 inhibitor SB203580 (HY-10256, MCE); JNK inhibitor SP600125 (HY-12041, MCE); MEK1/2 inhibitor U0126 (HY-12031, MCE); RhoA/C inhibitor (S7719, Selleck). ZAK inhibitors 6P and HY180 are gifts from Prof. Xiaoyun Lu, Jinan University. Antibodies used in this study include the following: anti-Cre (Rabbit, 15036T, CST); anti-HA (Rat, 11867423001, Roche); anti-HA (Rabbit, H6908, Sigma-Aldrich); anti-HA (Mouse, self-made); anti-SIK3 (Rabbit, self-made); anti-β-Tubulin (Mouse, HC101, TransGen Biotech); anti-ACTB (Mouse, 60008-1-Ig, Proteintech); anti-GAPDH (Mouse, 60004-1-Ig, Proteintech); anti-NeuN (Rabbit, 26975-1-AP, Proteintech); anti-Tau (Rabbit, 10274-1-AP, Proteintech); anti-MAP2 (Rabbit, 17490-1-AP, Proteintech); anti-ZAK (Rabbit, 28761-1-AP, Proteintech); anti-PKR (Rabbit, 18244-1-AP, Proteintech); anti-p-p38 (Rabbit, 4511, CST); anti-p-JNK (Rabbit, 4370, CST); anti-p-ERK1/2 (Rabbit, ET1609-42, HUABIO); HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H+L) (SA00001-2, Proteintech); HRP-conjugated Recombinant Rabbit Anti-Mouse IgG Kappa Light Chain (SA00001-1, Proteintech).

Li Y., Xu J., Guo X., Li Z., Cao L., Liu S., Guo Y., Wang G., Luo Y., Zhang Z., Wei X., Zhao Y., Liu T., Wang X., Xia H., Kuang M., Guo Q., Li J., Chen L., Wang Y., Li Q., Wang F., Liu Q, & You F. (2023). The collateral activity of RfxCas13d can induce lethality in a RfxCas13d knock-in mouse model. Genome Biology, 24, 20.

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Western Blot Analysis of Mouse Islet Proteins

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Protein extraction from primary mouse islets or INS-1 β-cells, protein concentration measurement, and Western blotting processes were performed as described before.^{[20 (link)]} Antibodies against α-tubulin (1:3000, M1501), BCL-2 associated X protein (BAX) (1:1000, ET1603-34), B-cell lymphoma 2 (BCL-2) (1:800, ET1603-11), p-Jun N-terminal kinase (JNK) (1:1500, ET1609-42), SOD2 (1:1200, ET1701-54), and GPX1 (1:800, ET1605-38) were purchased from HuaBio. Antibodies against NF-YA (1:1500, sc17753), NF-YB (1:800, sc-376546), and NF-YC (1:1200, sc-390985) were from Santa Cruz Biotechnology (Shanghai, China). Hybridized primary antibodies were detected with horseradish peroxidase (HRP)-labeled secondary antibodies. Protein signals were detected using the enhanced chemiluminescence reagents (Thermo Fisher Scientific, Waltham, MA, USA).

He S., Yu X., Cui D., Liu Y., Yang S., Zhang H., Hu W, & Su Z. (2023). Nuclear factor-Y mediates pancreatic β-cell compensation by repressing reactive oxygen species-induced apoptosis under metabolic stress. Chinese Medical Journal, 136(8), 922-932.

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