Log In Sign up
The largest database of trusted experimental protocols

Gtx121246

Manufactured by GeneTex
Visit Supplier

GTX121246 is a magnetic bead-based protein extraction kit designed for rapid and efficient purification of protein complexes from cell or tissue samples. The kit utilizes magnetic beads coated with specific antibodies or protein binding ligands to capture and isolate target proteins along with any associated binding partners. This product is suitable for a variety of downstream applications, such as protein interaction studies and mass spectrometry analysis.

Automatically generated - may contain errors

Lab products found in correlation

Ab191080, by Abcam (4 mentions) Ab192985, by Abcam (4 mentions) Ab15580, by Abcam (4 mentions) Ab202975, by Abcam (2 mentions) Ab138490, by Abcam (2 mentions) Ab195121, by Abcam (2 mentions) Ab181136, by Abcam (2 mentions) Flag tag (flag), by Merck Group (2 mentions) Vinculin, by Merck Group (2 mentions) Ab230445, by Abcam (2 mentions) Ab1791, by Abcam (2 mentions) Sc 7392, by Santa Cruz Biotechnology (2 mentions) Baf a1 s1413, by Selleck Chemicals (2 mentions) Sc 17775, by Santa Cruz Biotechnology (2 mentions) Sc 373822, by Santa Cruz Biotechnology (2 mentions) Vegfc, by ABclonal (2 mentions) F3165 1mg, by Merck Group (2 mentions) Myc tag, by Proteintech (2 mentions) Ha tag, by Proteintech (2 mentions) Gsk126, by Selleck Chemicals (2 mentions)

4 protocols using gtx121246

1

Comprehensive Antibody and Inhibitor Panel

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary antibodies used in this study are as follows: UTX (33510S, Cell Signaling Technology; GTX121246, GeneTex), COP1 (A300-894A, Bethyl), CUL1 (SC-17775, Santa Cruz Biotechnology), CUL2 (A5308, Abclonal), CUL3 (2759, Cell Signaling Technology), CUL4A (AB60215a, Abgent), CUL4B (A6198, Abclonal), CUL5 (SC-373822, Santa Cruz Biotechnology), EMP1 (ab230445 and ab202975, Abcam), AUTS2 (25,001–1-AP, Proteintech), Ki67 (ab15580, Abcam), EZH2 (ab191080, Abcam), VEGFC (A2556, Abclonal), Caspase-9 (A0281, Abclonal), Bcl2 (15,071, Cell Signaling Technology), DDB2 (ab181136, Abcam), DCAF7 (ab138490, Abcam), DCAF13 (ab195121, Abcam), H3K27me3 (ab192985, Abcam; A2363, Abclonal), H3 (ab1791, Abcam), P27 (610,241, BD), HA-Tag (51,064–2-AP, Proteintech; SC-7392, Santa Cruz Biotechnology), Flag-Tag (F7425-0.2MG, Sigma; F3165-1MG, Sigma), GFP (66,002–1-Ig, Proteintech), Myc-Tag (16,286–1-AP, Proteintech), α-tubulin (SC-23948, Santa Cruz Biotechnology), and Vinculin (V4505, Sigma). The chemical inhibitors MG132 (S2619), MG101 (S7386), MLN4924 (S7109), Baf-A1 (S1413), and GSK126 (S7061) were purchased from Selleck.
Luo D., Chen M., Li Q., Wang K., Wang K., Li J., Fu G., Shan Z., Liu Q., Yang Y., Liang L., Ma Y., Qin Y., Qin J., Gao D, & Li X. (2023). CUL4B-DDB1-COP1-mediated UTX downregulation promotes colorectal cancer progression. Experimental Hematology & Oncology, 12, 77.
+ Open protocol
+ Expand
2

Immunohistochemical Profiling of UTX and COP1

Check if the same lab product or an alternative is used in the 5 most similar protocols
IHC staining was performed with indicated antibodies using the standard protocol. The TMA was incubated with an anti-UTX antibody (1:400 dilution, 33510S, Cell Signaling Technology) and an anti-COP1 antibody (1:100 dilution, A300-894A, Bethyl). Mouse tissues were incubated with anti-UTX antibody (1:100 dilution, GTX121246, GeneTex), anti-COP1 antibody (1:100 dilution, A300-894A, Bethyl), Ki67 (1:100 dilution, ab15580, Abcam), anti-EZH2 antibody (1:200 dilution, ab191080, Abcam), and anti-H3K27me3 antibody (1:100 dilution, ab192985, Abcam; 1:100 dilution, A2363, Abclonal), respectively, as indicated. The IHC stained tissue sections were scored by two independent pathologists blinded to the clinicopathological features. The proportion of staining was graded as 0 (< 5%), 1 (5–25%), 2 (26–50%), 3 (51–75%), and 4 (> 75%) based on the percentages of the positive staining areas vs. the whole area. The staining intensity was graded as 0 (negative), 1 (weak), 2 (medium,) or 3 (strong). The immunoreactivity score (IRS) was calculated by multiplying the score of staining proportion with staining intensity. IRS ≤ 4 was considered as low, and > 4 was considered as high.
Luo D., Chen M., Li Q., Wang K., Wang K., Li J., Fu G., Shan Z., Liu Q., Yang Y., Liang L., Ma Y., Qin Y., Qin J., Gao D, & Li X. (2023). CUL4B-DDB1-COP1-mediated UTX downregulation promotes colorectal cancer progression. Experimental Hematology & Oncology, 12, 77.
+ Open protocol
+ Expand
3

Comprehensive Antibody and Inhibitor Panel

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary antibodies used in this study are as follows: UTX (33510S, Cell Signaling Technology; GTX121246, GeneTex), COP1 (A300-894A, Bethyl), CUL1 (SC-17775, Santa Cruz Biotechnology), CUL2 (A5308, Abclonal), CUL3 (2759, Cell Signaling Technology), CUL4A (AB60215a, Abgent), CUL4B (A6198, Abclonal), CUL5 (SC-373822, Santa Cruz Biotechnology), EMP1 (ab230445 and ab202975, Abcam), AUTS2 (25,001–1-AP, Proteintech), Ki67 (ab15580, Abcam), EZH2 (ab191080, Abcam), VEGFC (A2556, Abclonal), Caspase-9 (A0281, Abclonal), Bcl2 (15,071, Cell Signaling Technology), DDB2 (ab181136, Abcam), DCAF7 (ab138490, Abcam), DCAF13 (ab195121, Abcam), H3K27me3 (ab192985, Abcam; A2363, Abclonal), H3 (ab1791, Abcam), P27 (610,241, BD), HA-Tag (51,064–2-AP, Proteintech; SC-7392, Santa Cruz Biotechnology), Flag-Tag (F7425-0.2MG, Sigma; F3165-1MG, Sigma), GFP (66,002–1-Ig, Proteintech), Myc-Tag (16,286–1-AP, Proteintech), α-tubulin (SC-23948, Santa Cruz Biotechnology), and Vinculin (V4505, Sigma). The chemical inhibitors MG132 (S2619), MG101 (S7386), MLN4924 (S7109), Baf-A1 (S1413), and GSK126 (S7061) were purchased from Selleck.
Luo D., Chen M., Li Q., Wang K., Wang K., Li J., Fu G., Shan Z., Liu Q., Yang Y., Liang L., Ma Y., Qin Y., Qin J., Gao D, & Li X. (2023). CUL4B-DDB1-COP1-mediated UTX downregulation promotes colorectal cancer progression. Experimental Hematology & Oncology, 12, 77.
+ Open protocol
+ Expand
4

Immunohistochemical Profiling of UTX and COP1

Check if the same lab product or an alternative is used in the 5 most similar protocols
IHC staining was performed with indicated antibodies using the standard protocol. The TMA was incubated with an anti-UTX antibody (1:400 dilution, 33510S, Cell Signaling Technology) and an anti-COP1 antibody (1:100 dilution, A300-894A, Bethyl). Mouse tissues were incubated with anti-UTX antibody (1:100 dilution, GTX121246, GeneTex), anti-COP1 antibody (1:100 dilution, A300-894A, Bethyl), Ki67 (1:100 dilution, ab15580, Abcam), anti-EZH2 antibody (1:200 dilution, ab191080, Abcam), and anti-H3K27me3 antibody (1:100 dilution, ab192985, Abcam; 1:100 dilution, A2363, Abclonal), respectively, as indicated. The IHC stained tissue sections were scored by two independent pathologists blinded to the clinicopathological features. The proportion of staining was graded as 0 (< 5%), 1 (5–25%), 2 (26–50%), 3 (51–75%), and 4 (> 75%) based on the percentages of the positive staining areas vs. the whole area. The staining intensity was graded as 0 (negative), 1 (weak), 2 (medium,) or 3 (strong). The immunoreactivity score (IRS) was calculated by multiplying the score of staining proportion with staining intensity. IRS ≤ 4 was considered as low, and > 4 was considered as high.
Luo D., Chen M., Li Q., Wang K., Wang K., Li J., Fu G., Shan Z., Liu Q., Yang Y., Liang L., Ma Y., Qin Y., Qin J., Gao D, & Li X. (2023). CUL4B-DDB1-COP1-mediated UTX downregulation promotes colorectal cancer progression. Experimental Hematology & Oncology, 12, 77.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!