Trib1

Cisplatin (CDDP), paclitaxel (Taxel), doxorubicin (Dox), Vinorelbine (NVB) and SAHA were obtained from Sigma (St. Louis, MO, USA). Belinostat was synthesized in Medicine Chemistry Laboratory at Shenyang Pharmaceutical University (purity >97%). The primary antibodies against HDAC1, HDAC3, HDAC6, Nanog, Oct4, Sox2, ERK, phosphor-ERK and β-actin were got from Cell Signaling Technology (Beverly, MA, USA). The primary antibodies against CD133 was obtained from Mitenyi Biotec. The primary antibodies against TRIB1, C/EBP-β, HDAC8 and Acetyl-p53 were purchased from Abcam (Cambridge, MA, USA). Antibodies to ALDH1A1, ALDH1A3, ALDH2, ALDH3A1, ALDH5A1 and ALDH7A1 were obtained from Novus Biologicals (Littleton, CO, USA). The Silencer Select Validated siRNA, including TRIB1, HDAC1 and C/EBP-β, were got from Life technologies (Gaithersburg, MD, USA).

Proteins extracted from cell lysates and tissue lysates were separated by 10% SDS-PAGE and transferred onto PVDF membrane. The following primary antibodies were used: GAPDH (Kangchen Bio-tech, Shanghai, China), TRIB1 (Abcam), MMP2 (Proteintech), MMP9 (Proteintech), Phospho-FAK (Tyr397), Phospho-FAK (Tyr576/577), Phospho-FAK (Tyr925), FAK, Phospho-Src (Tyr416), Src, ERK1/2, Phospho-ERK1/2 (All from Cell Signaling Technology, USA). ERK inhibitor (SCH772984), Src inhibitor (PP2) and FAK inhibitor (PF562271) were obtained from Selleck Chemicals (Houston, TX, USA).