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4 protocols using sodium ionophore x

1

Electrochemical Sensor Development Protocol

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Polyvinyl chloride (PVC, selectophore, high molecular weight), agarose (low EEO), potassium tetrakis(4-chlorophenyl)borate (selectophore, ≥ 98%), sodium ionophore X (selectophore, function tested), α-d-glucose (anhydrous, 96%), pyruvic acid (98%), and AuNPs(20 nm diameter, stabilized suspension in citrate buffer) were supplied by Sigma-Aldrich Co., LLC (Japan). Phosphate-buffered saline (PBS(-), pH 7.1), 0.05 M Tris–HCl buffer solution (pH 7.6), tetrahydrofuran (THF, 99.5%, with stabilizer), potassium chloride (KCl, 99.5%), sodium chloride (NaCl, 99.5%) and ammonium chloride (NH4Cl, 99.5%), bis(2-Ethylhexyl) sebacate (DOS), magnesium chloride (MnCl2, 97%), urea (99%), and UA (98%) were purchased from FUJIFILM Waco Pure Chemical Corporation (Japan). Carbon grease (CG, particle size < 40 nm) was provided by KITACO Co., Ltd (Japan). Ag/AgCl ink for reference electrode was obtained from ALS Co., Ltd. PC plates were supplied by HAKUDO Corporation. CPE (MS-550) was purchased from Ecologa Japan Co., Ltd.
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2

Graphite-based Electrochemical Sensor Fabrication

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Graphite powder, β-CD, L-ascorbic acid, ammonium hydroxide, potassium chloride, sodium chloride, potassium tetrakis(pentafluorophenyl)borate (KTPFPB), sodium ionophore X, high molecular weight PVC, bis(2-ethylhexyl)sebacate (DOS), tetrahydrofuran (THF) and tridodecylmethylammonium chloride (TDMAC) were obtained from Sigma-Aldrich. Commercial conductive carbon ink (CC-ink) was procured from Henkel. All chemicals were analytical grade and used as received. Aqueous solutions were prepared in Milli-Q water (resistivity ~18 MΩ-cm).
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3

Graphite-based Electrochemical Sensor Fabrication

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Graphite powder, β-CD, L-ascorbic acid, ammonium hydroxide, potassium chloride, sodium chloride, potassium tetrakis(pentafluorophenyl)borate (KTPFPB), sodium ionophore X, high molecular weight PVC, bis(2-ethylhexyl)sebacate (DOS), tetrahydrofuran (THF) and tridodecylmethylammonium chloride (TDMAC) were obtained from Sigma-Aldrich. Commercial conductive carbon ink (CC-ink) was procured from Henkel. All chemicals were analytical grade and used as received. Aqueous solutions were prepared in Milli-Q water (resistivity ~18 MΩ-cm).
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4

Fabrication of Flexible Chemical Sensors

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EDOT, PSS, sodium ionophore X, bis(2-ethylehexyl) sebacate (DOS), PVB, polyvinyl chloride (PVC), sodium tetrakis[3,5-bis(trifluoromethyl)phenyl] borate (Na-TFPB), aniline, sodium thiosulfate pentahydrate (Na2S2O3), sodium bisulfite (NaHSO3), calcium chloride dihydrate (CaCl2·2H2O), block polymer PEO-PPO-PEO (F127), multiwall carbon nanotubes (MWCNTs), iron(III) chloride (FeCl3), potassium hydroxide (KOH), and citric acid were purchased from Sigma-Aldrich. Sodium chloride (NaCl), ammonium chloride (NH4Cl), methanol, ethanol, acetone, tetrahydrofuran (THF), hydrochloric acid (HCl), tetrachloroauric acid (HAuCl4), and disodium phosphate (Na2HPO4) were purchased from Thermo Fisher Scientific. PDMS (SYLGARD 184) was purchased from Dow Corning. Silver nitrate (AgNO3) was purchased from Alfa Aesar. Waterproof, double-sided medical tapes (75 μm thick) were purchased from Adhesives Research. Silver conductive paint was purchased from Structure Probe Inc. (SPI) supplies. Moisture-resistant PET film (100 μm thick) was purchased from McMaster-Carr. PTFE (50 μm thick) was purchased from JIAET.
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