Anti zo 1 cat

DMEM (Dulbecco’s modified Eagle’s medium)/F-12 medium, FBS (fetal bovine serum), and TRIzol were from Invitrogen (Carlsbad, CA, USA). The Hybond C membrane and ECL (enhanced chemiluminescence) Western blot detection system were from GE Healthcare Biosciences (Buckinghamshire, UK). The phospho-c-Src (Tyr416) (Cat. #6943), phospho-EGFR (Tyr845) (Cat. #2231), phospho-Akt (Ser473) (Cat. #4060), phospho-ERK1/2 (Thr202/Tyr204) (Cat. #4370), phospho-p38 (Thr180/Tyr182) (Cat. #4511), phospho-JNK1/2 (Thr183/Tyr185)(Cat. #4668), and phospho-p65 NF-κB (Ser536) (Cat. #3033) antibodies were from Cell Signaling (Danver, MA, USA). Anti-ZO-1 (Cat. #sc-33725) antibody was from Santa Cruz (Dallas, TX, USA). Anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase) antibody was from GeneTex (Irvine, CA, USA). MMP2/9 inhibitor (2/9i), apocynin, PP1, AG1478, SH-5, U0126, SB202190, SP600125, Bay11-7082, and were from Enzo (Farmingdale, NY, USA). BCA (Bicinchoninic acid) protein assay reagent was from Pierce (Rockford, IL, USA). IL-1β (interleuline-1β) was from R&D Systems (Minneapolis, MN, USA). NAC (N-acetyl-cysteine), enzymes, and other chemicals were from Sigma (St. Louis, MO, USA).

Dulbecco’s modified Eagle’s medium (DMEM)/F-12 medium, fetal bovine serum (FBS), and TRIzol were from Invitrogen (Carlsbad, CA, USA). The Hybond C membrane and enhanced chemiluminescence (ECL) Western blot detection system were from GE Healthcare Biosciences (Buckinghamshire, UK). The phospho-ERK1/2 (Thr202/Tyr204) (Cat.#4370), phospho-p38 (Thr180/Tyr182) (Cat.#4511), phospho-JNK1/2 (Thr183/Tyr185) (Cat.#4668), and phospho-p65 NF-κB (Ser536) (Cat.#3033) antibodies were from Cell Signaling (Danver, MA, USA). The anti-ZO-1 (Cat.#sc-33725) antibody was from Santa Cruz (Dallas, TX, USA). The anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody was from GeneTex (Irvine, CA, USA). The R-7050, U0126, SB202190, SP600125, Bay11-7082, and MMP2/9 inhibitor (2/9i) were from Enzo (Farmingdale, NY, USA). The bicinchoninic acid (BCA) protein assay reagent was from Pierce (Rockford, IL, USA). The tumor necrosis factor-α (TNF-α) was from R&D Systems (Minneapolis, MN, USA). The sophoraflavanone G (SG) was from ChemFaces (Wuhan, China). The enzymes and other chemicals were from Sigma (St. Louis, MO, USA).