To visualize GFP-tagged Msn2 in living cells, cells were grown at 25 °C until exponential phase and treated with 3 μg/ml tunicamycin (TM) for 3 h. Cells were then harvested, suspended in SD medium, and observed immediately using a Keyence BZ-X700 microscope (Keyence) with a PlanAproλ 100 × NA 1.45 oil objective lens (Nikon). Fluorescence intensities were quantified using Hybrid Cell Count BZ-H2C software (Keyence).
Planaproλ 100 na 1.45 oil objective lens
Manufactured by Nikon
Sourced in Japan
The PlanAproλ 100 × NA 1.45 oil objective lens is a high-performance microscope objective designed for use in laboratory and research applications. It features a magnification of 100x and a numerical aperture (NA) of 1.45, which provides excellent light-gathering capabilities and high-resolution imaging. The lens is optimized for use with oil immersion techniques.
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Lab products found in correlation
2 protocols using planaproλ 100 na 1.45 oil objective lens
1
Visualizing Msn2 Localization in Cells
2
Visualizing GFP-tagged Mig1 and Mig2
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To visualize GFP-tagged Mig1 and Mig2 in living cells, cells were grown at 25 °C until exponential phase and treated with 3 μg/ml tunicamycin (TM) for 3 hr. Cells were then harvested, suspended in SD medium, and observed immediately using a Keyence BZ-X700 microscope (Keyence Corporation, Japan) with a PlanAproλ 100× NA 1.45 oil objective lens (Nikon). Fluorescence intensities were quantified using Hybrid Cell Count BZ-H2C software (Keyence Corporation, Japan).
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