Endogenously TM4SF5-expressing liver-cancer cell lines (Huh7 and HepG2 cells) were purchased from the Korean Cell Line Bank (Seoul, Korea). HEK293FT cells were purchased from Thermo Fisher Scientific (R7007, Waltham, MA, USA). These cells were cultured in DMEM (SH30243.01, Hyclone, Logan, UT, USA) supplemented with 10% FBS (F0600, GenDEPOT, Barker, TX, USA) and penicillin/streptomycin (CA005, GenDEPOT) at 37 °C in a 5% CO2 environment. All cell lines were sub-cultured every three days and routinely monitored for mycoplasma contamination.
Ca005
Manufactured by GenDEPOT
Sourced in United States
The CA005 is a general-purpose centrifuge designed for a variety of laboratory applications. It features a compact and durable construction, providing reliable performance. The centrifuge can accommodate a range of sample sizes and tube types, making it a versatile tool for various laboratory workflows.
Automatically generated - may contain errors
Lab products found in correlation
Cm002, by GenDEPOT (3 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions)
F0600, by GenDEPOT (1 mentions)
Hek293ft cells, by Thermo Fisher Scientific (1 mentions)
Hepg2, by Korean Cell Line Bank (1 mentions)
Balb 5023, by Cell Biologics (1 mentions)
E2759, by Merck Group (1 mentions)
Cm002 050, by GenDEPOT (1 mentions)
Crl 2468, by American Type Culture Collection (1 mentions)
Fbs 22a, by Capricorn (1 mentions)
Rpmi a, by Capricorn (1 mentions)
Mcf 7, by Korean Cell Line Bank (1 mentions)
Dmem f12 medium, by Welgene (1 mentions)
I0516, by Merck Group (1 mentions)
6 protocols using ca005
1
Culturing Liver Cancer Cell Lines
2
Microglia-Conditioned Media Effects on Brain Endothelial Cells
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BV2 murine microglia (CRL-2468, ATCC, Manassas, VA, USA) and BALB/C mouse primary brain microvascular ECs (BALB-5023, Cell biologics, Chicago, IL, USA) were cultured with a medium containing high glucose (CM002-050, GenDEPOT, Katy, TX, USA) 10% fetal bovine serum (F0901-050, GenDEPOT) and penicillin-streptomycin (CA005, GenDEPOT) at 37 °C in a 5% CO2 humidified incubator. The medium for BALB/C mouse primary brain microvascular ECs was added EC growth supplement (E2759, Sigma-Aldrich). The cells were seeded in 6-well plates (5 × 104 cells/well)and treated with recombinant mouse sENG protein (1320-EN-025, R&D Systems) for 24 h. The sENG-treated BV2-conditioned media (sENG-BV2-CM) were mixed with EC medium (50% v/v) and incubated on the ECs.
3
Cell Culture and Transfection Protocol
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HEK293A and HepG2 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; CM002, GenDEPOT, Katy, TX) supplemented with 10% (v/v) fetal bovine serum (FBS; WB0015, HyClone, Logan, UT), 100 U/mL penicillin, and 100 μg/mL streptomycin (CA005, GenDEPOT, Katy, TX). The cells were cultured in a humidified incubator with 95% air and 5% CO2 at 37 °C. The DNA plasmids were transfected into the cells using Lipofectamine 3000 (Invitrogen, Carlsbad, CA), according to the manufacturer’s instruction.
4
Differentiation of Bone Marrow-Derived Macrophages
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BMDMs were differentiated from bone marrow progenitors isolated from the tibia and femur bones of C57BL/6 mice (6- to 12-weeks-old; Narabio Co., Seoul, Republic of Korea) in L929 cell-conditioned medium (LCCM) containing macrophage colony-stimulating factor. In brief, progenitors were plated in non-tissue culture-treated dishes (SPL Life Science Co., Gyeonggi-do, Republic of Korea) and incubated at 37 °C in a 5% CO2 atmosphere for 7 days. The cells were supplied with RPMI 1640 medium (RPMI-A, Capricorn Scientific GmbH, Edsdorfergrund, Germany) containing 10% fetal bovine serum (FBS; FBS-22A, Capricorn Scientific GmbH), 30% LCCM, and antibiotics (100 U/ml of penicillin and 100 µg/ml of streptomycin; CA005, GenDEPOT, Inc., Barker, TX, USA).
5
Breast Cancer MCF-7 Cell Culture
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Breast cancer Michigan Cancer Foundation-7 (MCF-7; Korean Cell Line Bank, Seoul, Republic of Korea) cells were used for all experiments. Dulbecco’s modified Eagle’s medium (DMEM; CM002, GenDEPOT, Katy, TX, USA) supplemented with 10% (v/v) fetal bovine serum (FBS; WB0015, HyClone, Logan, UT, USA) and 1% (v/v) penicillin–streptomycin (P/S) solution (100X; 100 units/mL of penicillin and 100 μg/mL of streptomycin; CA005, GenDEPOT, Katy, TX, USA) was used for cell maintenance. Cells were incubated at 37 °C in humidified conditions with 95% air and 5% CO2.
6
Cell Line Culture and Transfection Protocols
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The MCF-7, SiHa, and BeWo cell lines were purchased from the Korean Cell Line Bank (KCLB; Seoul, Republic of Korea), and HEK293A and HeLa cell lines were provided by Dr. Jihye Seong (Korea Institute of Science and Technology, Seoul, South Korea). The MCF-7 cells were cultured in RPMI-1640 medium (CM058; GenDEPOT, Barker, TX, United States) supplemented with 10% (v/v) fetal bovine serum (FBS; WB0015; HyClone), 100 U/mL penicillin, 100 μg/ml streptomycin (CA005, GenDEPOT), and 0.01 mg/ml insulin solution from bovine pancreas (I0516, Sigma). The HEK293A, HeLa, and SiHa cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; CM002, GenDEPOT) containing 10% (v/v) FBS, 100 U/ml penicillin, and 100 μg/ml streptomycin. The BeWo cells were cultured in DMEM/F-12 medium (LM002-08, Welgene) supplemented with 10% (v/v) FBS, 100 U/ml penicillin, and 100 μg/ml streptomycin. The cells were cultured in a humidified incubator with 95% air and 5% CO2 at 37°C. The DNA plasmids were transfected into the cells using Lipofectamine 3000 (L3000, Invitrogen) following the manufacturer’s protocol.
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