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Cd34 clone qbend 10

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CD34 (clone QBEnd/10) is a laboratory equipment product used for the identification and analysis of CD34-positive cells. It is a cell surface glycoprotein that serves as a marker for hematopoietic stem and progenitor cells. The product is designed for use in flow cytometry applications.

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Lab products found in correlation

Myogenin, by R&D Systems (1 mentions) Diaminobenzidine, by Agilent Technologies (1 mentions) Sox10, by R&D Systems (1 mentions) Beta catenin, by Cell Marque (1 mentions) Smooth muscle actin clone 1a4, by Merck Group (1 mentions) S100 protein clone poly z311, by Agilent Technologies (1 mentions) Myod1 clone ep212, by Cell Marque (1 mentions) Benchmark ultra automated stainer, by Roche (1 mentions) H3k27me3 clone c36b11, by Cell Signaling Technology (1 mentions) Ki 67 clone 30 9, by Roche (1 mentions) Benchmark ultra platform, by Roche (1 mentions) Optiview dab ihc detection kit, by Roche (1 mentions) Benchmark ultra immunostainer, by Roche (1 mentions) Gfap z0334, by Agilent Technologies (1 mentions) Clone mib 1, by Agilent Technologies (1 mentions) Neun clone a60, by Merck Group (1 mentions) Ultraview universal dab detection kit, by Roche (1 mentions)

4 protocols using cd34 clone qbend 10

1

Immunohistochemistry for Diagnostic Markers

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Hematoxylin and eosin-stained slides and immunohistochemical slides were reviewed in all cases. In cases with unstained slides or paraffin blocks available, additional 4-μm-thick sections were obtained for immunohistochemistry. The following antibodies were performed systematically in a subset of cases using automated instruments SOX10 (clone N-20; Santa Cruz Biotechnology, Dallas, TX; 1:100 dilution), CD34 (clone QBEnd/10; Ventana, Tucson, AZ; prediluted) and H3 K27 trimethylation (clone C36B11, Cell Signaling Technology, Danvers, MA; 1:100 dilution).
Costa F.D., Dias T.M., Lombardo K.A., Raghunathan A., Giannini C., Kenyon L., Saad A.G., Gokden M., Burger P.C., Montgomery E.A, & Rodriguez F.J. (2019). Intracranial Cellular Schwannomas: A Clinicopathologic Study of 20 Cases. Histopathology, 76(2), 275-282.
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2

Immunohistochemical Staining Panel for Sarcoma

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The tissue slides were deparaffinized in xylene, hydrated in alcohol, and baked in a microwave (30 min in Trisbuffer, pH 9). Endogenous peroxidase was blocked. Staining was performed on the Benchmark ultra-automated stainer (Ventana) using diamino-benzidine as chromogen (Dako, Glostrup, Denmark). The following antibodies were used: Pankeratin AE1/AE3 (clone PCK26, pre-diluted; Ventana Media Systems, Tucson, AZ ,USA); EMA (clone E29, pre-diluted; Ventana Media Systems, Tucson, Arizona ,USA); S100 protein (clone poly Z311, dilution 1:500; Dako, Glostrup, Denmark); smooth muscle actin (clone 1A4, dilution 1:12000; Sigma Aldrich, Saint Louis, MO, USA); desmin (clone DE-R-11, pre-diluted; Ventana Media Systems, Tucson, Arizona USA); myogenin (clone LO 26, dilution 1:20; Leica Biosystems, Buffalo Grove, IL, USA); MyoD1 (clone EP212, pre-diluted; Cell Marque, Rocklin, CA, USA); CD34 (clone QBEnd10, pre-diluted; Ventana, Tucson, AZ, USA); beta-catenin (clone 14, pre-diluted; Cell Marque, Rocklin, CA, USA); SOX10 (clone EP268, dilution 1:100; BioSB, Santa Barbara, CA, USA); H3K27Me3 (clone C36B11, dilution 1:200; Cell Signaling Technology, Danvers, MA, USA); PAX3 (clone 274212, dilution 1:100; RD Systems Europe, Lille, France)
For myogenin, MyoD1, SOX10, H3K27me3 beta-catenin and PAX3, only a nuclear staining was considered as positive.
Le Loarer F., Laffont S., Lesluyes T., Tirode F., Antonescu C., Baglin A., Delespaul L., Soubeyran I., Hostein I., Pérot G., Chibon F., Baud J., Le Guellec S., Karanian M., Costes-Martineau V., Castain C., Eimer S., Le Bail B., Wassef M, & Coindre J. (2019). Clinicopathological and molecular features of a series of 41 biphenotypic sinonasal sarcomas expanding their molecular spectrum. The American journal of surgical pathology, 43(6), 747-754.
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3

Histopathological Characterization of Solitary Fibrous Tumors

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All diagnoses were confirmed by a senior neuropathologist (PC) and reassessed according to the 2016 WHO classification. Thus, tumours were graded based on their phenotype (solitary fibrous tumour versus hemangiopericytoma) and mitotic count on H&E slides. Mitotic count was repeated after PHH3 IHC (polyclonal, Ventana Medical Systems Inc., Tucson, AZ, US). IHC for STAT6 (polyclonal, Spring Bioscience Corporation, Pleasanton, CA, US), Ki67 (clone 30–9, Ventana Medical Systems Inc., Tucson, AZ, US), CD34 (clone QBEnd/10, Ventana Medical Systems Inc., Tucson, AZ, US) and Bcl-2 (clone SP66, Ventana Medical Systems Inc., Tucson, AZ, US) were also performed. Immunohistochemistry for Ki67, CD34 and Bcl-2 was performed on all tissue blocks of each case; Ki67 labelling index was evaluated by counting at least 1000 tumour cells while CD34 and Bcl-2 were assessed by visual estimation. Nuclear staining for STAT6 was considered consistent with the SFT/HPC diagnosis. BenchMark ULTRA platform (Ventana Medical Systems Inc., Tucson, AZ, USA) was used for all IHC.
Bertero L., Anfossi V., Osella-Abate S., Disanto M.G., Mantovani C., Zenga F., Rudà R., Garbossa D., Soffietti R., Ricardi U., Papotti M, & Cassoni P. (2018). Pathological prognostic markers in central nervous system solitary fibrous tumour/hemangiopericytoma: Evidence from a small series. PLoS ONE, 13(9), e0203570.
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4

Histopathological Characterization of CNS Tumors

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All samples with available tissue (n = 24/30) were histopathologically re-assessed according to the WHO 2016 classification of tumors of the central nervous system. Formalin-fixed, paraffin-embedded (FFPE) tissue samples were stained with hematoxylin and eosin (H&E) according to standard protocols. For all cases with sufficient material, immunohistochemistry was performed on a Ventana BenchMark ULTRA Immunostainer using either the OptiView DAB IHC Detection Kit or the ultraView Universal DAB Detection Kit (Ventana Medical Systems, Tucson, Arizona, USA). Antibodies were directed against: glial fibrillary acid protein (GFAP; Z0334, rabbit polyclonal, 1:1000 dilution, Dako Agilent, Santa Clara, CA, USA), Olig2 (clone EPR2673, rabbit monoclonal, 1:100 dilution, Abcam, Cambridge, UK), Synaptophysin (clone MRQ-40, rabbit monoclonal, 1:160 dilution, Cell Marque Corp., Rocklin, CA, USA), NeuN (clone A60, mouse monoclonal, 1:100 dilution, Millipore, Burlington, MA, USA), CD34 (clone QBEnd/10, mouse monoclonal, Ventana Medical Systems), Ki-67 (clone MIB-1, mouse monoclonal, 1:100 dilution, Dako Agilent).
Sievers P., Appay R., Schrimpf D., Stichel D., Reuss D.E., Wefers A.K., Reinhardt A., Coras R., Ruf V.C., Schmid S., de Stricker K., Boldt H.B., Kristensen B.W., Petersen J.K., Ulhøi B.P., Gardberg M., Aronica E., Hasselblatt M., Brück W., Bielle F., Mokhtari K., Lhermitte B., Wick W., Herold-Mende C., Hänggi D., Brandner S., Giangaspero F., Capper D., Rushing E., Wesseling P., Pfister S.M., Figarella-Branger D., von Deimling A., Sahm F, & Jones D.T.W. (2019). Rosette-forming glioneuronal tumors share a distinct DNA methylation profile and mutations in FGFR1, with recurrent co-mutation of PIK3CA and NF1. Acta neuropathologica, 138(3).
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