1 3 dicyclohexylcarbodiimide dcc

1-Octadecene (ODE) and indium acetate (In(Ac)₃) were bought from TCI America. Zinc acetate (Zn(Ac)₂), myristic acid (MA), elemental sulfur, tris(trimethylsilyl)phosphine ((TMS)₃P), 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT), tris(2-carboxyethyl)phosphine hydrochloride (TCEP), lactic acid (LA), N,N′-dimethylformamide (DMF), 4′,6-diamidino-2-phenyl-indole (DAPI), 4-dimethylamino pyridine (DMAP), and bovine plasma were bought from Sigma-Aldrich. Heterobifunctional PEG derivatives (M_w = 5000 Da), HO-PEG-OCH₃, and HO-PEG-Mal from JenKem Technology were used for this study. 1,3-Dicyclohexylcarbo-diimide (DCC) was bought from ACROS. AF (NSC 686288, 4H-1-benzopyran-4-one,5-amino-2-(4-amino-3-fluorophenyl)-6,8-difluoro-7-methyl) was acquired from the repository of the Developmental Therapeutics Program (National Cancer Institute (NCI)) at Frederick. The anti-EGFR nanobody (Nb), 7D12, was kindly provided by Prof. Lei Liu’s laboratory at Tsinghua University, China. The buffers for refolding Nbs included the following: (1) renaturation buffer A, 10 mM phosphate-buffered saline (PBS) and 8 M urea, pH 7.4; (2) renaturation buffer B, 10 mM PBS and 6 M urea, pH 7.4; (3) renaturation buffer C, 10 mM PBS and 4 M urea, pH 7.4; (4) renaturation buffer D, 10 mM PBS and 2 M urea, pH 7.4; and (5) renaturation buffer E, 10 mM PBS, pH 7.4.

Boltron® H40 (a hyperbranched polyester with 64 hydroxyl terminal groups; M_n: 2833 Da) was kindly provided by Perstorp Polyols Inc., USA, and purified by fractional precipitation in acetone and tetrahydrofuran (THF). β-Benzyl L-aspartate N-carboxyanhydride (BLA-NCA) was prepared as previously reported by our lab [49 (link)]. The heterobifunctional poly(ethylene glycol) (PEG) derivatives, methoxy–PEG–NH₂ (CH₃O-PEG–NH₂, M_n = 5 kDa) and maleimide–PEG–NH₂ (Mal–PEG–NH₂, M_n = 5 kDa), were purchased from JenKem Technology (Allen, TX, USA). Cy5 dye was obtained from Lumiprobe Corporation (Hallandale Beach, FL, USA). GE11 peptide (YHWYGYTPQNVIGGGGC) was synthesized by Tufts University Core Facility (Boston, MA, USA). GFP-siRNA-Cy5.5, GFP-siRNA, dimethyl sulfoxide (DMSO), 2-carboxybenzylaldehyde, 2-aminoethyl disulfide, 4-imidazolecarboxylic acid, and stannous (II) octoate (Sn(Oct)₂) were purchased from Sigma–Aldrich (St. Louis, MO, USA). 4-Dimethylamino pyridine (DMAP) and 1,3-dicyclohexylcarbodiimide (DCC) were purchased from ACROS and used without further purification. Other reagents, including RNAiMAX, were purchased from Thermo Fisher Scientific (Fitchburg, WI, USA) and used as received unless otherwise stated.

Poly(amidoamine) (1,4-diaminobutane; G4) dendrimer was purchased from NanoSynthons LLC (Mt. Pleasant, MI, USA). Dimethyl sulfoxide (DMSO), velarolactone (VL), and stannous (II) octoate (Sn(Oct)₂) were purchased from Sigma–Aldrich (St. Louis, MO, USA). The heterobifunctional poly(ethylene glycol) (PEG) derivatives, methoxy–PEG–OH (mPEG–OH, M_n=5 kDa) and OH–PEG–N–hydroxylsuccinimide (HO–PEG–NHS, Mn=5 kDa) were purchased from JenKem Technology (Allen, TX, USA). 4-Dimethylamino pyridine (DMAP) and 1,3-dicyclohexylcarbodiimide (DCC) were purchased from ACROS and used without further purification. KE108 was purchased from Bachem Americas, Inc. (Torrance, CA, USA). Cy5 dye was obtained from Lumiprobe Corporation (Hallandale Beach, FL, USA). Other reagents were purchased from Thermo Fisher Scientific (Fitchburg, WI, USA) and used as received unless otherwise stated.