Krt6a

RPMI 1640 and fetal bovine serum (FBS) were obtained from HyClone Laboratories (HyClone Laboratories Inc.). Dimethyl-labeled agents, CH₂O, CD₂O, NaCNBH₃ were obtained from Sigma-Aldrich Company (St. Louis, MO). The primary antibodies for Hsp90 alpha, ENO1, ALDOA, HSPA5, JUP, KRT6A, PDIA6, AKT, PCNA, FN1, β-actin and all the secondary antibodies were obtained from Proteintech Group (Proteintech Group, Inc., USA).
All other agents were purchased from Sigma Chemical Co. (St. Louis, MO) unless otherwise specified. The water used in the experiments was thrice-distilled; all other materials were of analytical reagent grade.

Paraffin sections of PDAC tissue and ANT tissue were used for IHC staining. Sections were stained with primary antibodies against KRT6A (1:200, Proteintech Group, Chicago, IL, USA) and ITGAM (1:200, Proteintech Group, Chicago, IL, USA) according to the product manual after routine steps. Phosphate-buffered saline (PBS) was used as a control.
The scoring of positive immunoreactivity was performed. The intensity was classified as 0, 1+, 2+, and 3+, denoting no, weak, moderate, and strong staining, respectively. The distribution of staining was referred to as the percentage of positive tumor cells (0% to 100%). The final KRT6A and ITGAM expression scores were obtained by multiplying the two variables together.