Cell were washed with PBS and fixed with 4% paraformaldehyde supplemented with 0.25% Tris-X100 at room temperature for 10 min. After blocking with PBS supplemented with 5% BSA for 2 h at room temperature, cells were incubated with NOX-1 (1:200, ab55831, Abcam), EBP50 (1:1000, 3394, Abcam or 1:200, sc-271552, Santa Cruz Biotechnology), p47phox (1:200, ab166930, Abcam or 1:200, sc-17845, Santa Cruz Biotechnology) at 4° C overnight. Cells were incubated with secondary peroxidase conjugated goat anti-rabbit IgG (1:100, Santa Cruz Biotechnology) antibody for 2 h at room temperature, after washing with PBST for 15 min. Cells were stained with DAPI for 15 min at darkness, after washing with PBST for 15 min. Cell samples were observed using fluorescence microscope (Zeiss Axio Observer A1, Germany).
Secondary peroxidase conjugated goat anti rabbit igg
Manufactured by Santa Cruz Biotechnology
Secondary peroxidase conjugated goat anti-rabbit IgG is a laboratory reagent used to detect the presence of rabbit immunoglobulin G (IgG) in biological samples. The product consists of goat-derived antibodies that are chemically conjugated to the enzyme peroxidase. This conjugate can be used in various immunoassay techniques, such as Western blotting and enzyme-linked immunosorbent assay (ELISA), to amplify and visualize the signal associated with rabbit IgG.
Automatically generated - may contain errors
Lab products found in correlation
Axio observer a1, by Zeiss (6 mentions)
Ab166930, by Abcam (1 mentions)
Ab55831, by Abcam (1 mentions)
Sc 271552, by Santa Cruz Biotechnology (1 mentions)
Sc 17845, by Santa Cruz Biotechnology (1 mentions)
Mito tracker red cmxros, by Beyotime (1 mentions)
Mitotracker red cmxros, by Zeiss (1 mentions)
2 protocols using secondary peroxidase conjugated goat anti rabbit igg
1
Immunofluorescence Staining of NOX-1, EBP50, and p47phox
2
Fluorescent Microscopic Analysis of Mitochondrial Function and Oxidative Stress
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Cell were washed with PBS and fixed with 4% paraformaldehyde supplemented with 0.25% Tris-X100 at room temperature for 10 min. After blocking with PBS supplemented with 5% BSA for 2 h at room temperature, cells were incubated with Mito-Tracker Red CMXRos (C1035, Beyotime), NLRP3 (sc-66846, 1:500, Santa Cruz, USA), at 4° C overnight. Cells were incubated with secondary peroxidase conjugated goat anti-rabbit IgG (1:100, Santa Cruz Biotechnology) antibody for 2 h at room temperature, after washing with PBST for 15 min. Cells were stained with DAPI for 15 min at darkness, after washing with PBST for 15 min. Cell samples were observed using fluorescence microscope (Zeiss Axio Observer A1, Germany).
Next, cells were washed with PBS and stained with Mito-Tracker Red CMXRos for 30 min. Cells washed with PBST for 15 min and stained with DCFH-DA for 30 min. Cell samples were observed using fluorescence microscope (Zeiss Axio Observer A1, Germany) after washing with PBST for 15 min.
Cell were washed with PBS and fixed with 4% paraformaldehyde for 15 min. Cells were stained with PI for 15 min at darkness, after washing with PBST for 15 min. Cell samples were observed using fluorescence microscope (Zeiss Axio Observer A1, Germany).
Next, cells were washed with PBS and stained with Mito-Tracker Red CMXRos for 30 min. Cells washed with PBST for 15 min and stained with DCFH-DA for 30 min. Cell samples were observed using fluorescence microscope (Zeiss Axio Observer A1, Germany) after washing with PBST for 15 min.
Cell were washed with PBS and fixed with 4% paraformaldehyde for 15 min. Cells were stained with PI for 15 min at darkness, after washing with PBST for 15 min. Cell samples were observed using fluorescence microscope (Zeiss Axio Observer A1, Germany).
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