Bactec bottles

Manufactured by BD

Sourced in United States

The BACTEC bottles are laboratory containers designed for the detection and culturing of microorganisms in clinical samples. They provide a standardized environment for the growth and identification of various types of bacteria, fungi, and other pathogens.

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Lab products found in correlation

Bactec 9050 blood culture system, by BD (2 mentions) Bactec fx40, by BD (1 mentions) Maldi tof ms, by Bruker (1 mentions) Bactec fx blood culture system, by BD (1 mentions)

10 protocols using bactec bottles

Coaxial Needle Biopsy and Aspiration

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The procedure was similar to that described by Moore.6 (link) Under the guidance of helical multidetector row CT fluoroscopy, biopsies were performed using a coaxial technique with a 19-gauge thin-wall coaxial introducer needle, and core biopsies (CBs) were performed with an 18-gauge automated cutting needle biopsy gun (FINECORE; Dr.Japan Co., Ltd.). After CB, NA was performed. A 10 mL syringe was connected with a thin-wall outer needle, moved up and down slightly and 2–5 mL (according to the nodule size) of secretion was drawn from the diseased tissue. The obtained tissue was fixed on formalin and sent for pathological examination.
The liquid obtained from the aspiration was inoculated into BACTEC bottles (Becton, Dickinson and Company, Franklin Lakes, NJ, USA) for bacterial, fungal and mycobacterial culture (Figure S1). The samples were also placed on a glass slide for cytological and microbiological tests.
The detailed procedure is shown in Video S1. Chest X-rays were obtained after the procedure to detect possible pulmonary complications.

Zhou Y., Lin P.C., Ye J.R., Su S.S., Dong L., Wu Q., Xu H.Y., Xie Y.P, & Li Y.P. (2018). The performance of serum cryptococcal capsular polysaccharide antigen test, histopathology and culture of the lung tissue for diagnosis of pulmonary cryptococcosis in patients without HIV infection. Infection and Drug Resistance, 11, 2483-2490.

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Specimen Collection Protocol for Infectious Diseases

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High vaginal swabs were taken from the posterior fornix before pelvic examination after separating the labia with the left hand.

Swabs for bacterial culture were ten from the pus of cesarean incision, infected episiotomy wound; pus was aspirated with a syringe in cases of pyoperitoneum where laparotomy was carried out.

Blood (10 mL) was collected aseptically for blood culture from a peripheral vein and transferred equally in two Becton Dickinson, Towson, Md (BACTEC) bottles with aerobic and anaerobic media and was sent to the microbiology department.

Midstream urine samples were collected and sent for culture.

Singh P., Tirkey S., Trivedi K., Hansda R, & Prakash J. (2022). Study of cases of puerperal sepsis, its socio-demographic factors, bacterial isolates, and antibiotic sensitivity pattern. Journal of Family Medicine and Primary Care, 11(9), 5155-5160.

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Staphylococcus aureus Bacteremia Detection

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We collected 1–3-mL blood samples from all patients with suspected septicemia, pneumonia, or meningitis; inoculated blood samples into BACTEC bottles (Becton Dickinson, https://www.bd.com); and incubated them in an automated BACTEC 9050 Blood Culture System (Becton Dickinson) for a maximum of 5 days. We subcultured positive cultures on blood agar plates and confirmed isolates as S. aureus by using catalase and coagulase tests. We classified cultures that grew Bacillus spp., Corynebacterium spp., and coagulase-negative Staphylococcus as contaminated. We used standard methods to investigate other body fluid samples collected for microbiological tests (20 (link)). We used disc diffusion methods to determine antimicrobial drug susceptibility according to the Clinical and Laboratory Standards Institute guidelines (21 ). We categorized all S. aureus isolates resistant to cefoxitin as methicillin-resistant.
We defined S. aureus bacteremia cases as clinically suspected cases of septicemia, pneumonia, meningitis, osteomyelitis, septic arthritis, pyomyositis, or abscess identified by using standardized criteria (19 (link)) in patients from whom S. aureus was isolated from their blood.

Odutola A., Bottomley C., Zaman S.A., Lindsay J., Shah M., Hossain I., Ndiaye M., Osuorah C.D., Olatunji Y., Badji H., Ikumapayi U.N., Manjang A., Salaudeen R., Ceesay L., Jasseh M., Adegbola R.A., Corrah T., Hill P.C., Greenwood B.M, & Mackenzie G.A. (2019). Staphylococcus aureus Bacteremia in Children of Rural Areas of The Gambia, 2008–2015. Emerging Infectious Diseases, 25(4), 701-709.

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Comprehensive Microbial Investigation for Meningitis

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Blood or CSF, or both, were collected for conventional microbiological investigations. Blood was inoculated into BACTEC bottles (Becton Dickinson) and incubated in an automated BACTEC 9050 blood culture system (Becton Dickinson) for a maximum of 5 days. Positive cultures were sub-cultured on blood and chocolate agar and examined for bacterial growth following 24 or 48-hours of aerobic incubation, incubation in 5-10% CO 2 and anaerobic incubation, all at 37 o C. Similarly, CSF was cultured on blood and chocolate agar and isolates identified by biotyping, and serotyping for pneumococcal serotypes. Cell count, Gram-stain and bacterial antigen (latex agglutination) tests were performed on CSF according to WHO procedures (WHO/CDS/CSR/EDC/99.7). Rapid diagnostic tests for malaria using ICT-Malaria p.f. Antigen tests (ICT Diagnostics) were performed routinely during the malaria transmission season. Children with clinical signs of meningitis but who had a positive rapid ICT diagnostic test for malaria and normal leukocyte count were excluded from our analysis.

Ikumapayi U.N., Hill P.C., Hossain I., Olatunji Y., Ndiaye M., Badji H., Manjang A., Salaudeen R., Ceesay L., Adegbola R.A., Maiden M., & Mackenzie G. (2022). Childhood meningitis in rural Gambia: 10 years of population-based surveillance.

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Microbial Identification and Antimicrobial Susceptibility Testing

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All clinical specimens, except for blood, were inoculated on 5% Colombia Sheep Blood, Chocolate, and MacConkey agars and incubated aerobically at 35–37°C for 18–22 h. Blood samples were collected and added to BD BACTEC bottles (Becton Dickinson, Sparks, MD, USA) and then incubated for 5 days at 35–37°C in the BD BACTEC™ FX40 (Becton Dickinson, Sparks, MD, USA) automated culture machine. If growth was observed, it was sub-cultured on 5% Colombia Sheep Blood, Chocolate, and MacConkey agars in similar environmental conditions for further analysis. Subsequently, all positive pure cultures were tested for antimicrobial susceptibility. Isolates were picked off the plates and kept at −80°C in storage media containing skimmed milk, tryptone soya, glucose, glycerol, and distilled water until they were transported to Max von Pettenkofer Institute, Hospital Hygiene, and Medical Microbiology Laboratory in Munich, Germany. There, the isolates were re-identified using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS, Bruker, Germany).

Gashaw M., Gudina E.K., Ali S., Gabriele L., Seeholzer T., Alemu B., Froeschl G., Kroidl A, & Wieser A. (2024). Molecular characterization of carbapenem-resistance in Gram-negative isolates obtained from clinical samples at Jimma Medical Center, Ethiopia. Frontiers in Microbiology, 15, 1336387.

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Automated Blood Culture Diagnostics

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Clinical blood cultures generated in BD Bactec bottles (Becton, Dickinson and Company, USA) were collected from the department of Medical Microbiology (University Medical Center Groningen [UMCG]) after one to two weeks of culturing and storage. All blood culture samples were incubated until marked positive by a BD BACTEC FX Blood Culture System, or until unloaded as culture-negative after 4–7 days. Each blood sample was sub-cultured in parallel according to standard procedures to verify the microbiological diagnosis by plating and MALDI-TOF mass spectrometry.

López-Álvarez M., Heuker M., Schoenmakers J.W., van Dam G.M., McNamara JO I.I., van Dijl J.M, & van Oosten M. (2020). The smart activatable P2&3TT probe allows accurate, fast, and highly sensitive detection of Staphylococcus aureus in clinical blood culture samples. Scientific Reports, 10, 19216.

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Bacterial Isolation and Identification

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Samples were collected by aseptic technique and sent to the hospital laboratory where culture was performed on nutrient agar, blood agar, MacConkey agar, chocolate agar and cysteine lactose electrolyte deficient agar plates; all the blood samples were first inoculated in BACTEC™ bottles (BD, Franklin Lakes, NJ, USA), followed by sub-culture on solid media. Colonies were sub-cultured for purity and identified by colony morphology and biochemical tests according to standard procedures.21

Koju P., Liu X., Zachariah R., Bhattachan M., Maharjan B., Madhup S., Shewade H.D., Abrahamyan A., Shah P., Shrestha S., Li H, & Shrestha R. (2021). Incidence of healthcare-associated infections with invasive devices and surgical procedures in Nepal. Public Health Action, 11(Suppl 1), 32-37.

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Sterility Evaluation of Cell-Derived EVs

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The sterility of EV samples that were isolated from cell culture media was evaluated. Samples (100 µL) were inoculated in liquid media (BACTEC bottles; BD) and monitored for aerobic or anaerobic bacterial growth (14 days). Sterility testing was performed on three cell culture media-derived EV samples isolated from separate TFF runs.

Busatto S., Vilanilam G., Ticer T., Lin W.L., Dickson D.W., Shapiro S., Bergese P, & Wolfram J. (2018). Tangential Flow Filtration for Highly Efficient Concentration of Extracellular Vesicles from Large Volumes of Fluid. Cells, 7(12), 273.

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Validating Multiplex ddPCR Assay

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To validate the multiplex ddPCR assay, the analytical sensitivity and specificity will be compared to the blood culture technique and the BCID assay. For this study, a total of 50 blood samples (two BD BACTEC bottles corresponding to 20 mL and 1 mL of whole blood) from healthy/noninfected donors will be used. A total of 48 samples will be spiked with S aureus, S pneumoniae, E coli, and P aeruginosa (12 samples per bacteria) in triplicates of each concentration (ie, 1, 5, 10, and 10² CFU/mL). Two samples will be cultured with saline as nontemplate controls. All samples will be analyzed in duplicate by the multiplex ddPCR and BCID assay in parallel with blood cultures.

Badran S., Chen M, & Coia J.E. (2021). Multiplex Droplet Digital Polymerase Chain Reaction Assay for Rapid Molecular Detection of Pathogens in Patients With Sepsis: Protocol for an Assay Development Study. JMIR Research Protocols, 10(12), e33746.

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Microbial Contamination Monitoring in CAR T-Cell Therapy

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BACTEC bottles (aerobic and anaerobic; BD Biosciences, San Jose, CA) were inoculated at multiple process steps with 500–1,000 μL cellular product obtained from the apheresis material, on day 7 and after the final formulation of the CAR T cells in NaCl with 0.5% HSA, before cryopreservation. Microbiological control was carried out according to European Pharmacopeia (EP) 2.6.27 at the Experimental and Clinical Research Center (ZKP, Charité-University Medicine Berlin, Berlin, Germany) using the BD BACTEC Model 9050.

Joedicke J.J., Großkinsky U., Gerlach K., Künkele A., Höpken U.E, & Rehm A. (2021). Accelerating clinical-scale production of BCMA CAR T cells with defined maturation stages. Molecular Therapy. Methods & Clinical Development, 24, 181-198.

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