Int bcip brown kit

Manufactured by Sangon

The INT/BCIP (Brown) kit is a laboratory reagent used for the detection and visualization of alkaline phosphatase enzyme activity in various biological applications. The kit contains the necessary components to produce a brown-colored reaction product, allowing for the identification and localization of alkaline phosphatase-positive cells or tissues.

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Lab products found in correlation

Celltiter blue kit, by Promega (1 mentions) Synergy neo2, by Agilent Technologies (1 mentions) Celltiter blue cell viability assay kit, by Promega (1 mentions)

2 protocols using int bcip brown kit

Monitoring mESC Viability and Alkaline Phosphatase

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mESCs were seeded into 96 well plates in growth medium for 0 h, 24 h, 48 h, 72 h, and 96 h, respectively. CellTiter-Blue kit (Promega, Cat. #G8081) was used to measure cell viability at different time points. Fluorometric signals were recorded by a microplate reader (BioTek, Synergy NEO2).
mESCs were fixed with 1% paraformaldehyde before alkaline phosphatase staining. The activity of alkaline phosphatases in mESCs was detected by INT/BCIP (Brown) kit (Sangon Biotech, Cat. #C500033) following the manufacturer’s instructions. NIH3T3 cells were used as negative controls.

Zhang Y., Fang Y., Tang Y., Han S., Jia J., Wan X., Chen J., Yuan Y., Zhao B, & Fang D. (2022). SMYD5 catalyzes histone H3 lysine 36 trimethylation at promoters. Nature Communications, 13, 3190.

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Measuring Cell Viability and Staining

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500 cells were seeded into each well of a 96-well plate, and the cell viability was measured after 0, 24, 48, 72 and 96 h, respectively by CellTiter-Blue cell viability assay kit (Promega, Cat. #G8081).
10⁶ mESCs were plated in each well of a 24-well plate. Cells were fixed with 1% paraformaldehyde for 5 min at room temperature and then were stained by INT/BCIP (Brown) kit (Sangon Biotech, Cat.# C500033-0005) for 15 min at 37°C. T/C28a2 cells were used as the negative controls.

Fang Y., Tang Y., Zhang Y., Pan Y., Jia J., Sun Z., Zeng W., Chen J., Yuan Y, & Fang D. (2021). The H3K36me2 methyltransferase NSD1 modulates H3K27ac at active enhancers to safeguard gene expression. Nucleic Acids Research, 49(11), 6281-6295.

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