Phosphor irs 1 ser307

Manufactured by Cell Signaling Technology

Sourced in United States

Phosphor-IRS-1 (Ser307) is a lab equipment product that detects the phosphorylation of insulin receptor substrate 1 (IRS-1) at serine 307. It is used to measure the activation status of this specific site within the IRS-1 protein.

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Lab products found in correlation

3 protocols using phosphor irs 1 ser307

Insulin Signaling Pathway Regulation

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Antibodies against TXNIP (#14715), Akt (#2920), phosphor-Akt (Thr308) (#4056), AS160 (#2670), phosphor-AS160 (Ser588) (#8730), PDK1 (#13037), GLUT4 (#2213), PERK (#3192), IRE1α (#3294), CHOP (#5554), ATF6 (#65880), Histone H3 (#9715), IRS-1 (#2382), phosphor-IRS-1 (Ser307), JNK (#9252), phosphor-JNK (Thr183/Tyr185) (#4668), NF-κB p65 (#4764), phosphor-NF-κB p65 (Ser536) (#3033), and IκBα (#9242) were from Cell Signaling TECHNOLOGY (Beverly, MA, United States). Anti-PERK (phosphor T982) (ab192591), STAT3 (ab119352), STAT3 (phosphor Y705) (ab76315), and SOCS3 (ab16030) antibodies were obtained from Abcam, Inc. (Cambridge, MA, United States). Anti-IL-6 mouse monoclonal antibody (sc-57315) and normal mouse IgG (sc-2025) were purchased from Santa Cruz Biotechnology (Shanghai) Co., Ltd. (Shanghai, China). Insulin (91077C), palmitic acid (P5585), and icariin (I1286) were acquired from Sigma-Aldrich, Corp. (St. Louis, MO, United States). 2-Deoxy-D-2-[3H] glucose was obtained from HTA, Co. Ltd. (Beijing, China).

Li M., Zhang Y., Cao Y., Zhang D., Liu L., Guo Y, & Wang C. (2018). Icariin Ameliorates Palmitate-Induced Insulin Resistance Through Reducing Thioredoxin-Interacting Protein (TXNIP) and Suppressing ER Stress in C2C12 Myotubes. Frontiers in Pharmacology, 9, 1180.

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Insulin Signaling Pathway Regulation

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Palmitic Acid-Induced HepG2 Cell Model

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Human hepatoma cell line HepG2 was obtained from the cell bank of Chinese Academy of Sciences (Shanghai, China). Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Genom, Hangzhou, China) supplemented with 10% fetal bovine serum (Hyclone, Victoria, Australia) and 1% penicillin & streptomycin (Biological Industries, Cromwell, CT, USA) at 37 °C in a humidified incubator with 5% CO₂. Palmitic acid (PA) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Mdivi-1 was bought from Meilunbio, Inc. (Hangzhou, China). The primary antibodies against GLUT4, Akt, phosphor-Akt (Ser473), IRS-1, phosphor-IRS-1 (Ser307) and GAPDH were obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA).

Tong T., Ren N., Soomi P., Wu J., Guo N., Kang H., Kim E., Wu Y., He P., Tu Y, & Li B. (2018). Theaflavins Improve Insulin Sensitivity through Regulating Mitochondrial Biosynthesis in Palmitic Acid-Induced HepG2 Cells. Molecules, 23(12), 3382.

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