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Chromogenic substrates

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Sourced in Spain, Italy

Chromogenic substrates are laboratory reagents used in various analytical techniques. They are designed to produce a colored reaction product upon interaction with specific enzymes or chemical agents, enabling the detection and quantification of target analytes. These substrates provide a visual indication of the presence or activity of the analyte of interest, facilitating the analysis and interpretation of experimental results.

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Lab products found in correlation

Bovine fibrinogen, by Merck Group (2 mentions) Peptone, by Helicon (1 mentions) Hide powder azure, by Merck Group (1 mentions) Casein, by Merck Group (1 mentions)

2 protocols using chromogenic substrates

1

Preparation of Inorganic Compounds for Research

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All inorganic compounds and starch were purchased from Chimmed Company, Russia. Moreover, peptone and fish flour hydrolysate were obtained from Helicon Company, Russia. All chromogenic substrates were purchased from Chromogenix Company, Spain. In addition, bovine fibrinogen was purchased from Merck Company, USA, and human blood plasma was purchased from Renam company, Russia.
Surkova D., Lavrenova V., Klyagin S., Shestakova A, & Osmolovsky A. (2023). Screening of proteases produced by Aspergillus micromycetes active against proteins of the hemostasis system. Current Medical Mycology, 9(1), 8-13.
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2

Enzymatic Activity Characterization Protocol

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Chromogenic substrates were obtained from Chromogenix AB (Milano, Italy). Molecular mass standards for SDS-PAGE were from Bio-Rad Laboratories Ltd. (California, USA). Purified factor Xa, bovine alpha thrombin, one chain t-PA (sctPA), Two chains u-PA (tcu-PA) and plasmin (used for controls), were obtained from American Diagnostica Inc. (Greenwich, CT, USA). Bovine fibrinogen (10% w/w of plasminogen as contaminant) was obtained from Sigma (St. Louis, MO, USA). Synthetic substrates, casein, hide powder azure, serine protease inhibitor cocktail, Na-Benzoyl-L-Arginine Ethyl Ester (BAEE) and other reagents used in this study were obtained from Sigma Chemical Co (St. Louis, MO, USA).
Girón M.E., Padrón V., Ramos M.I., Sánchez E.E., Guerrero B., García A., Uzcátegui N.L., Navarrete L.F, & Rodríguez-Acosta A. (2018). Intraspecies geographical variability in the South American tigra mariposa (Bothrops venezuelensis Sandner 1952) snake venom activities. Toxicon : official journal of the International Society on Toxinology, 144, 23-33.
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