Sphingosine 1 phosphate (s1p)

Manufactured by Fujifilm

Sourced in Japan

The S1P is a versatile lab equipment product from Fujifilm. It is designed to perform essential functions required in various laboratory settings. The core function of the S1P is to provide reliable and consistent results for the tasks it is intended for. Further details on the specific capabilities and intended use of this product are not available at this time.

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Lab products found in correlation

Sphingosine 1 phosphate (s1p), by Enzo Life Sciences (1 mentions) Vpc23019, by Avanti Polar Lipids (1 mentions) Fatty acid free bsa, by Merck Group (1 mentions) Pertussis toxin ptx, by Fujifilm (1 mentions) D erythro s1p, by Enzo Life Sciences (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Methanol, by Fujifilm (1 mentions) Sphingosine 1 phosphate (s1p), by Merck Group (1 mentions)

2 protocols using sphingosine 1 phosphate (s1p)

Sphingolipid Signaling Molecules Analysis

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Sphingosine 1-phosphate, and D-erythro (S1P) were purchased from Enzo Life Sciences (Plymouth Meeting, PA). C₁₇-Sphingosine 1-phosphate (C₁₇-S1P), lysophosphatidic acid (LPA), lysophosphatidylserine (LPS), lysophosphatidylinositol (LPI), and VPC23019 were purchased from Avanti Polar Lipids Inc (Alabaster, AL). Pertussis toxin (PTX) was obtained from Wako Pure Chemical Industries (Osaka, Japan).
S1P, LPA, LPS and LPI were dissolved in methanol. Just before use, the methanol was evaporated and the reagents were resolved in PBS containing 0.4% fatty acid-free BSA (Sigma-Aldrich Co., St. Louis, MO). C₁₇-S1P was dissolved in methanol. VPC23019 and PTX were dissolved in DMSO.

Iino J., Osada M., Kurano M., Kaneko M., Ohkawa R., Satoh Y., Okubo S., Ozaki Y., Tozuka M., Tsuno N.H, & Yatomi Y. (2014). Platelet-derived sphingosine 1-phosphate induces migration of Jurkat T cells. Lipids in Health and Disease, 13, 150.

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Inhibition of Sphingosine Kinases by SKi

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2-(p-hydroxyanilino)-4-(p-chlorophenyl)thiazole (SKi, an Sk inhibitor that inhibits both Sk1 and Sk2) and Sph were purchased from Echelon (Salt Lake City, UT, USA). S1P was purchased from Sigma. SKi, Sph and S1P were tested at 10-50 μM. SKi was initially dissolved in dimethyl sulfoxide (DMSO; Sigma) to 10 mM and stored at -20 •C. Sph and S1P were initially dissolved in methanol (Wako Pure Chemical Industries, Osaka, Japan) to 10mM, and 2 mM, respectively, and stored at -20 •C. Each agent was diluted in the culture medium to the final concentration immediately before use. The final concentrations of DMSO were 14-70 mM in SKi.

Tani K., Tabuchi K, & Hara A. (2016). Hair Cell Loss Induced by Sphingosine and a Sphingosine Kinase Inhibitor in the Rat Cochlea. Neurotoxicity research, 29(1).

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