Rhtnfα

BT lines from glioma patients (BT25, BT114, BT116, BT132) were obtained as described previously [31 (link)]. 293 T, U87-MG, and U373 cells were purchased from ATCC. Glioma cell lines were cultured in DMEM/F12 + 10%FBS (1X Glutamax, 1X Pen/Strep) or Neural Stem Cell (NSC) medium (DMEM/F12, 1X B-27 Supplement minus Vitamin A, 1X Glutamax, 50 ng/ml EGF, 50 ng/ml bFGF, 1X Pen/Strep). All cell culture reagents were from Life Technologies (Grand Island, NY). rhTNFα was obtained from Promega (Madison, WI), and rhTWEAK was obtained from PeproTech (Rocky Hill, NJ). GM6001 inhibitor and MMP Inhibitor I were obtained from EMD Millipore (Temecula, CA).

Collagen type I was isolated and prepared as previously described^{52 (link)} or purchased from Corning, Corning, NY, USA (#354249). rhTNFα (#G5241) was obtained from Promega, Madison, WI, USA; rhTWEAK (#310-06) was purchased from PeproTech, Rocky Hill, NJ, USA; TAPI-2 was obtained from Calbiochem, San Diego, CA, USA. MG132 was purchased from Cell Signaling Technology, Danvers, MA, USA (#2194S). Anti-cortactin was purchased from Upstate, Lake Placid, NY, USA (# 05-180); anti-p65 (SC-8008) and anti-RelB (SC-226) were from Santa Cruz Biotechnology, Dallas, TX, USA; anti-pMT1-MMP-Y573 was custom-generated from 21st Century Biochemicals, Marlboro, MA, USA,^{21 (link), 53 (link), 54 (link)} anti-MT1-MMP was from EMD Millipore, Billerica, MA, USA (MAB3328); anti-α-Actin was from Calbiochem (CP01); AF488 Phalloidin (A12379) and AF-647 Phalloidin (A22287) were purchased from ThermoFisher Scientific/Life Technologies/Invitrogen (Grand Island, NY, USA), anti-NFKB2/p100/p52 (CST4882), p-p65-Ser536 (CST3033) and NIK (#4994, for immunoblot) were purchased from Cell Signaling; anti-GAPDH (ab8245) and anti-NIK (ab7204, for immunofluorescence) were purchased from Abcam, Cambridge, MA, USA. DAPI was purchased from Invitrogen (#D1306).