4 methylthio butyl itc 4 mtb itc

2-propenyl glucosinolate hydrate (sinigrin, allyl-GLS, ≥ 99%), 2-propenyl isothiocyanate (allyl-ITC, ≥ 99%), benzonitrile (≥ 99.9%), 3-butenenitrile (allyl-CN, ≥ 98%), thioglucosidase (myrosinase) from Sinapis alba (white mustard) seed (EC 3.2.1.147), 2-phenylethyl isothiocyanate (2-phenylethyl-ITC, ≥ 99%), and 3-phenylpropanenitrile (2-phenylethyl-CN, ≥ 99%) were purchased from Sigma-Aldrich Chemie GmbH (Steinheim, Germany). 4-(Methylthio)butyl ITC (4-MTB-ITC; ≥ 98%) was purchased from Santa Cruz Biotechnology (Heidelberg, Germany); 1-cyano-2,3-epithiopropane (CETP, ≥ 95%) was purchased from Taros Chemicals GmbH & Co. KG (Dortmund, Germany); methylene chloride (GC Ultra Grade) from Carl Roth GmbH (Karlsruhe, Germany); acetonitrile (Ultra Gradient HPLC grade) from J.T. Baker (Deventer, The Netherlands); iron standard (1 g/L Fe, (FeCl₃ in 15% HCl) Titrisol), HNO₃ (65%, Suprapur) and H₂O₂ (30%, Suprapur) were purchased from Merck KGaA (Darmstadt, Germany). NaSO₄ (≥ 99%) was purchased from VWR International GmbH (Darmstadt, Germany). Water was of Milli-Q quality.

2Prop-ITC, (≥99%), benzonitrile (≥99.9%), 3-butenenitrile (2Prop-CN, ≥98%), 4-pentenenitrile (3But-CN, ≥97%), 3-phenylpropanenitrile (2PE-CN, ≥99%) and sucrose-sodium nitrate media were purchased from Sigma-Aldrich Chemie GmbH, Steinheim, Germany; IAN, (≥98%) from Acros Organics (Fischer Scientific GmbH, Schwerte, Germany); 3-butenyl ITC (3But-ITC, ≥95%) and 4-pentenyl ITC (4Pent-ITC, ≥95%) were purchased from TCI Deutschland GmbH, Eschborn, Germany; 3-hydroxypropionitrile was purchased from Thermo Fischer Scientific, Erembodegem, Belgium; 4-(methylthio)butyl ITC (4MTB-ITC, ≥98%) was purchased from Santa Cruz Biotechnology, Heidelberg, Germany; 5-(methylsulfinyl)pentyl ITC (5MSOP-ITC) was purchased from Enzo Life Sciences GmbH, Lörrach, Germany; 1-cyano-2,3-epithiopropane (CETP), (≥95%) was purchased from Taros Chemicals GmbH Co. KG, Dortmund, Germany; 4-hydroxybenzyl GLS (≥97%), methylene chloride (GC Ultra Grade), Tris, EDTA, NaCl, CTAB, chloroform/isoamylalcohol (24:1), β-mercaptoethanol and phenol/chloroform/isoamylalcohol (25:24:1) from Carl Roth GmbH, Karlsruhe, Germany; acetonitrile (Ultra Gradient HPLC grade) was purchased from J.T. Baker, Deventer, The Netherlands and NaSO₄ (≥99%) and methanol (>99.9) were purchased from VWR International GmbH, Darmstadt, Germany. Potato dextrose agar was purchased from Merck, Darmstadt, Germany. All solvents were of LC or GC-MS grade.