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Aspartate transaminase

Manufactured by Randox
Sourced in United Kingdom

Aspartate transaminase is a laboratory test that measures the level of the enzyme aspartate transaminase in the blood. Aspartate transaminase is an enzyme found in various tissues, including the liver, heart, and muscles. This test is used to help detect and monitor certain medical conditions that can cause tissue damage or dysfunction.

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4 protocols using aspartate transaminase

1

Investigating Hepatoprotective Effects

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Lead acetate, TUR, and Vit-C were purchased from Sigma Chemical Co. (Sigma, St.
Louis, Missouri, USA). Commercial kits for the assay of alanine transaminase
(ALT), aspartate transaminase (AST), lactate dehydrogenase (LDH) were purchased
from (Randox Laboratories Ltd, Crumlin, United Kingdom). Primary antibodies for
BCL-2-associated X (Bax) and B-cell CLL/lymphoma 2 (Bcl-2) were purchased from
(Santa Cruz Biotechnology, Santa Cruz, California). Secondary antibodies were
purchased from Sigma-Aldrich.
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2

Analysis of Medicinal Plant Compounds

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Fresh samples of the sclerotia of P. tuberregium were purchased from Mile 1 Market in Port Harcourt, Nigeria, while fresh leaves of C. aconitifolius (hospital too far) were collected from Farm Gardens in Aluu Community of Rivers State, Nigeria, and were duly identified as previously reported (Ikewuchi and Ikewuchi, 2009 , 2011 ; Ikewuchi et al., 2013a (link),b , 2014 (link), 2017 (link); Ifeanacho et al., 2019a (link),b , 2020 (link)). Ninety Wistar rats (weight 80–130 g) were obtained from the Animal House of the Department of Physiology, University of Port Harcourt, Nigeria. The alanine transaminase, aspartate transaminase, alkaline phosphatase, creatinine, and urea kits were the products of Randox Laboratories Ltd., County Antrim, UK.
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3

Hepatotoxicity Evaluation Protocol

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Acetaminophen, chloroform, and methanol used were obtained from Fam-lab Nigeria Limited, Akure, and Lixok-k chemicals, Akure, respectively. Alanine transaminase (ALT), aspartate transaminase (AST), gamma glutamyltransferase (GGT) and total protein, and bilirubin were obtained from Randox Laboratories Limited, UK.
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4

Biochemical Assays for Liver and Kidney Evaluation

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All chemicals and reagents were of analytical grade and were used as supplied. Assay kits for alkaline phosphate (ALP), aspartate transaminase (AST), alanine transaminase (ALT), creatinine (CREA), urea, albumin (ALB), and bilirubin (BIL) were products of Randox Laboratories Limited (Crumlin, UK).
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