For co-housing experiments, age-matched SFB-absent mice (from Jackson
Laboratory) were co-housed with SFB-present mice (from Taconic Biosciences) in
sterilized cages for two weeks at a ratio of 2:3, with unrestricted access to
food and water. For SFB-gavaging experiments, four fecal pellets of SFB
mono-colonized mice (provided by Dan Littman) were dissolved in 20 ml sterile
PBS and filtered though a 100 μm cell strainer. 200 μl of fecal
suspensions were gavaged via oral route to 4 week-old female Jackson mice.
Control mice were gavaged with PBS. The SFB colonization was tested on day 7
following co-housing or SFB-gavaging. For ablation of intestinal bacteria,
Taconic-derived female mice were orally gavaged with vancomycin hydrochloride
(Fisher) (2.5 mg/kg) every two days, starting 7 days prior to breeding. Mouse
fecal pellets were collected and stored at −80 °C before and
after vancomycin treatments.
Laboratory) were co-housed with SFB-present mice (from Taconic Biosciences) in
sterilized cages for two weeks at a ratio of 2:3, with unrestricted access to
food and water. For SFB-gavaging experiments, four fecal pellets of SFB
mono-colonized mice (provided by Dan Littman) were dissolved in 20 ml sterile
PBS and filtered though a 100 μm cell strainer. 200 μl of fecal
suspensions were gavaged via oral route to 4 week-old female Jackson mice.
Control mice were gavaged with PBS. The SFB colonization was tested on day 7
following co-housing or SFB-gavaging. For ablation of intestinal bacteria,
Taconic-derived female mice were orally gavaged with vancomycin hydrochloride
(Fisher) (2.5 mg/kg) every two days, starting 7 days prior to breeding. Mouse
fecal pellets were collected and stored at −80 °C before and
after vancomycin treatments.