Ec plan neofluar 20 lens

Manufactured by Zeiss

The EC Plan NeoFluar 20× lens is a high-quality optical lens designed for use in various laboratory applications. It provides a 20× magnification and is part of the NeoFluar series from Zeiss, known for their exceptional optical performance and reliability.

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Lab products found in correlation

Axiovert 200m microscope, by Zeiss (2 mentions) Axiovision 4, by Zeiss (2 mentions) Glass bottoms, by MatTek (1 mentions)

2 protocols using ec plan neofluar 20 lens

Time-lapse Imaging of T3-treated Cells

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A2780 and HCT116 cells (each, 5.0 × 10⁴ per well) were cultured in the wells of 24-well culture plates and treated with T3 or DMSO. The treated cells were incubated for 3 h at 37°C under an atmosphere of 5% CO₂/95% air in a microscope stage-mounted humidified chamber (Incubator XL S1; Carl Zeiss AG, Oberkochen, Germany). Serial phase-contrast images were captured every 10 min using an Axiovert 200M microscope (Carl Zeiss AG) equipped with an EC Plan NeoFluar 20× lens (Carl Zeiss AG) and processed using AxioVision 4.5 software (Carl Zeiss AG), then compiled in JPEG format and exported to Adobe Photoshop for processing.

Murai A., Ebara S., Sasaki S., Ohashi T., Miyazaki T., Nomura T, & Araki S. (2020). Synergistic apoptotic effects in cancer cells by the combination of CLK and Bcl-2 family inhibitors. PLoS ONE, 15(10), e0240718.

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Time-lapse Imaging of Transfected HeLa Cells

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HeLa cells were cultured in 24-well culture plates with glass bottoms (MatTek Corp., Ashland, MA, USA) and transfected with siRNA oligos targeting the indicated genes. Phase-contrast images were captured every 10 min for 24 h (12–36 h after transfection). Cells were imaged using an Axiovert 200M microscope equipped with EC Plan NEOFLUAR × 20 lens (Carl Zeiss). Images were acquired and processed using AxioVision 4.5 software (Carl Zeiss). The images were converted to JPEG format and exported to Adobe Photoshop.

Ohashi A., Ohori M., Iwai K., Nakayama Y., Nambu T., Morishita D., Kawamoto T., Miyamoto M., Hirayama T., Okaniwa M., Banno H., Ishikawa T., Kandori H, & Iwata K. (2015). Aneuploidy generates proteotoxic stress and DNA damage concurrently with p53-mediated post-mitotic apoptosis in SAC-impaired cells. Nature Communications, 6, 7668.

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