Anti tigit clone mbsa43

Manufactured by Thermo Fisher Scientific

Anti-TIGIT– clone MBSA43 is a monoclonal antibody that binds to the TIGIT (T-cell Immunoreceptor with Ig and ITIM Domains) receptor. TIGIT is an inhibitory receptor expressed on various immune cells, including T cells, NK cells, and regulatory T cells. The Anti-TIGIT– clone MBSA43 can be used for research purposes to study the role of TIGIT in immune regulation and immune cell function.

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Lab products found in correlation

Viability dye, by Thermo Fisher Scientific (1 mentions) Anti cd3 clone ucht1, by Thermo Fisher Scientific (1 mentions) Anti cd8 clone rpa t8, by Thermo Fisher Scientific (1 mentions) Anti ki67 clone 20raj1, by Thermo Fisher Scientific (1 mentions) Lsrfortessa flow cytometer, by BD (1 mentions) Fc block, by BD (1 mentions) Foxp3 transcription factor staining buffer set, by BD (1 mentions) Rainbow beads, by Spherotech (1 mentions) Human fc block, by BD (1 mentions) Interleukin 4 (il 4), by BD (1 mentions) Anti pd 1 clone j116, by Thermo Fisher Scientific (1 mentions) Anti tim 3 clone f382e2, by BioLegend (1 mentions) Cd14 cells, by STEMCELL (1 mentions) E coli lps, by Merck Group (1 mentions) Gm csf, by Miltenyi Biotec (1 mentions)

Close spelling variants of this product

Anti-TIGIT (MBSA43, TIGIT (MBSA43, Anti-TIGIT MBSA43

2 protocols using anti tigit clone mbsa43

Multiparameter Flow Cytometry of PBMC

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Viable frozen peripheral blood mononuclear cells were incubated with Fc block (BD Biosciences) prior to staining for surface markers (anti-CD3-clone UCHT1, anti-CD4–clone RPA-T4, anti-CD8–clone RPA-T8, anti-4-1BB–clone 4B4-1, anti-TIGIT– clone MBSA43, anti-Ki67–clone 20Raj1) and viability dye (eBioscience). Cells were fixed and permeabilized for intercellular staining with the Foxp3 transcription factor staining buffer set (BD). Flow cytometry voltages were set using Rainbow beads (Spherotech) with the same setting between experiments. Samples were acquired on a BD LSR Fortessa flow cytometer and data were analyzed using the FlowJo software.

Das A., Sudhaman S., Morgenstern D., Coblentz A., Chung J., Stone S.C., Alsafwani N., Liu Z.A., Karsaneh O.A., Soleimani S., Ladany H., Chen D., Zatzman M., Cabric V., Nobre L., Bianchi V., Edwards M., Sambira Nahum L.C., Ercan A.B., Nabbi A., Constantini S., Dvir R., Yalon-Oren M., Campino G.A., Caspi S., Larouche V., Reddy A., Osborn M., Mason G., Lindhorst S., Bronsema A., Magimairajan V., Opocher E., De Mola R.L., Sabel M., Frojd C., Sumerauer D., Samuel D., Cole K., Chiaravalli S., Massimino M., Tomboc P., Ziegler D.S., George B., Van Damme A., Hijiya N., Gass D., McGee R.B., Mordechai O., Bowers D.C., Laetsch T.W., Lossos A., Blumenthal D.T., Sarosiek T., Yen L.Y., Knipstein J., Bendel A., Hoffman L.M., Luna-Fineman S., Zimmermann S., Scheers I., Nichols K.E., Zapotocky M., Hansford J.R., Maris J.M., Dirks P., Taylor M.D., Kulkarni A.V., Shroff M., Tsang D.S., Villani A., Xu W., Aronson M., Durno C., Shlien A., Malkin D., Getz G., Maruvka Y.E., Ohashi P.S., Hawkins C., Pugh T.J., Bouffet E, & Tabori U. (2022). Genomic predictors of response to PD-1 inhibition in children with germline DNA replication repair deficiency. Nature Medicine, 28(1), 125-135.

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Monocyte-derived Dendritic Cell Activation

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Monocyte derived dendritic cells (DCs) were produced after selection of CD14+ cells (Stem Cell Technologies) from PBMCs isolated from a Buffy Coat, using the manufacturer’s instructions. CD14+ monocytes were cultured for 5 days in 7.5 ng/ml IL-4 (BD) and 20 ng/mL GMC-SF (Miltenyi Biotec). On day 4, 1 ng/ml of E. coli LPS (Sigma) was added to the cultures to mature the dendritic cells. Previously frozen PBMCs from patients and healthy controls were thawed and co-cultured with allogeneic DCs at 1:10 DCs to PBMCs with 5 μg/ml of blocking antibodies: anti-PD-1 (Clone J116, eBioScience), anti-TIGIT (Clone MBSA43, eBioScience), anti-TIM-3 (Clone F38-2E2, BioLegend), or Isotype control (Clone P3.6.2.8.1, eBioScience) in the presence of human Fc Block (BD). Cultures were kept at 37°C and 5% CO₂ for five days and supernatants were collected and stored at −80°C.

Fleury M., Belkina A.C., Proctor E.A., Zammitti C., Simms R.W., Lauffenburger D.A., Snyder-Cappione J.E., Lafyatis R, & Dooms H. (2018). INCREASED EXPRESSION AND MODULATED REGULATORY ACTIVITY OF CO-INHIBITORY RECEPTORS PD-1, TIGIT AND TIM-3 IN LYMPHOCYTES OF SYSTEMIC SCLEROSIS PATIENTS. Arthritis & rheumatology (Hoboken, N.J.), 70(4), 566-577.

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